Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

The determination of enzyme inhibitor constants

1953 • 4,050 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

1983 • 2,456 citations

Chemistry and biology of pteridines

1956 • 1,263 citations

Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.

1980 • 903 citations

Kinetics of the reversible inhibition of enzyme-catalysed reactions by tight-binding inhibitors

1969 • 779 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate.

1982 • 737 citations

[23] Buffers of constant ionic strength for studying pH-dependent processes

1982 • 644 citations

Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure

1987 • 508 citations

Isolation and expression of an altered mouse dihydrofolate reductase cDNA.

1983 • 436 citations

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

1978 • 426 citations

Refined crystal structures of Escherichia coli and chicken liver dihydrofolate reductase containing bound trimethoprim.

1985 • 273 citations

Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase

1967 • 235 citations

Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. II. Environment of bound NADPH and implications for catalysis.

1982 • 234 citations

Functional Role of Aspartic Acid-27 in Dihydrofolate Reductase Revealed by Mutagenesis

1986 • 233 citations

A new way of enhancing the thermostability of proteases

1986 • 226 citations

Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase

1979 • 223 citations

Crystal structure of avian dihydrofolate reductase containing phenyltriazine and NADPH.

1982 • 219 citations

Structure and genomic organization of the mouse dihydrofolate reductase gene

1980 • 218 citations

Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts.

1981 • 212 citations

Directed Mutagenesis of Dihydrofolate Reductase

1983 • 210 citations

Dihydrofolate reductase. The stereochemistry of inhibitor selectivity.

1985 • 139 citations

Further Studies on Dihydrofolic Reductase

1963 • 138 citations

Inhibition of dihydrofolate reductase: effect of NADPH on the selectivity and affinity of diaminobenzylpyrimidines

1982 • 94 citations

Kinetic mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli

1982 • 91 citations

The structure of mouse L1210 dihydrofolate reductase‐drug complexes and the construction of a model of human enzyme

1987 • 89 citations

Mechanism of inhibition of dihydrofolate reductases from bacterial and vertebrate sources by various classes of folate analogues

1986 • 86 citations

Phenotypic expression in Escherichia coli and nucleotide sequence of two Chinese hamster lung cell cDNAs encoding different dihydrofolate reductases.

1984 • 63 citations

Dihydrofolate reductase hysteresis and its effect on inhibitor binding analyses

1981 • 59 citations

Rapid synthesis of trideoxyribonucleotide blocks

1978 • 59 citations

Catalytic mechanism of the dihydrofolate reductase reaction as determined by pH studies

1984 • 59 citations

Bovine liver dihydrofolate reductase. Purification and properties of the enzyme

1975 • 57 citations

Purification and characterization of dihydrofolate reductase from methotrexate-resistant human lymphoblastoid cells

1983 • 53 citations

Mismatch-stimulated killing.

1986 • 52 citations

The amino acid sequence of dihydrofolate reductase from the mouse lymphoma L1210.

1979 • 52 citations

Inhibition of dihydrofolate reductase from bacterial and vertebrate sources by folate, aminopterin, methotrexate and their 5-deaza analogues

1984 • 50 citations

Porcine liver dihydrofolate reductase. Purification, properties, and amino acid sequence.

1979 • 48 citations

Importance of a hydrophobic residue in binding and catalysis by dihydrofolate reductase.

1986 • 45 citations

The pH-dependence of the binding of dihydrofolate and substrate analogues to dihydrofolate reductase from Escherichia coli

1983 • 43 citations

Regulatory mutants of dihydrofolate reductase in Escherichia coli K12

1976 • 36 citations

Site‐specific mutagenesis of dihydrofolate reductase from Escherichia coli

1985 • 33 citations

Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli

1985 • 24 citations

Amplification and expression of human alpha-globin genes in Chinese hamster ovary cells.

1984 • 18 citations

Tight binding inhibitors—IX

1981 • 17 citations

Expression of the mouse dihydrofolate reductase cDNA inB.subtilis: a system to select mutant cDNAs coding for methoirexate resistant enzymes

1984 • 12 citations