Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

1949 • 19,979 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Selected methods in cellular immunology

1981 • 1,915 citations

Chemistry and biology of pteridines

1956 • 1,263 citations

The Biochemistry of Folic Acid and Related Pteridines.

1970 • 1,033 citations

Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

1978 • 972 citations

ANALYSIS OF CELL POPULATIONS WITH A FLUORESCENCE‐ACTIVATED CELL SORTER*

1975 • 405 citations

Specific Binding of 4-Amino Folic Acid Analogues by Folic Acid Reductase

1961 • 389 citations

Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes.

1979 • 376 citations

Dihydrofolate Reductase: X-ray Structure of the Binary Complex with Methotrexate

1977 • 323 citations

Enzyme Inhibition in Relation to Chemotherapy.

1949 • 260 citations

Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells

1976 • 237 citations

Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase

1967 • 235 citations

Treatment of leukemia with large doses of methotrexate and folinic acid: clinical-biochemical correlates

1969 • 234 citations

Dihydrofolate reductase from Lactobacillus casei. X-ray structure of the enzyme methotrexate.NADPH complex.

1978 • 229 citations

Relation of Folic Acid Reductase to Amethopterin Resistance in Cultured Mammalian Cells

1961 • 210 citations

Studies on the Inhibition of Dihydrofolate Reductase by the Folate Antagonists

1964 • 176 citations

Synthesis and degradation of folate reductase in sensitive and methotrexate-resistant lines of S-180 cells.

1976 • 171 citations

Molecular Techniques and Approaches in Developmental Biology

1973 • 143 citations

The Pharmacological Basis of Therapeutics

1976 • 142 citations

Further Studies on Dihydrofolic Reductase

1963 • 138 citations

Quantitation of dihydrofolate reductase in individual parental and methotrexate-resistant murine cells. Use of a fluorescence activated cell sorter

1978 • 131 citations

Purification and Properties of Dihydrofolate Reductase from Ehrlich Ascites Carcinoma Cells^*

1965 • 130 citations

Gene transfer in intact animals

1980 • 129 citations

On the nature of a transport alteration determining resistance to amethopterin in the L1210 leukemia.

1968 • 129 citations

Evaluation of methods for estimating the dissociation constant of tight binding enzyme inhibitors.

1979 • 111 citations

Dihydrofolate Reductase from a Resistant Subline of the L1210 Lymphoma

1967 • 109 citations

A rapid, radiochemical-ligand binding assay for methotrexate

1976 • 106 citations

Methotrexate-resistant Chinese hamster ovary cells contain a dihydrofolate reductase with an altered affinity for methotrexate

1980 • 106 citations

Dihydrofolate reductase from Lactobacillus caSci. Stereochemistry of NADPH binding.

1979 • 103 citations

Two different species of dihydrofolate reductase in mammalian cells differentially resistant to amethopterin and methasquin.

1972 • 101 citations

Acquired methotrexate resistance in lymphoblasts resulting from altered kinetic properties of dihydrofoltate reductase

1977 • 95 citations

Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei

1972 • 93 citations

THE “INDUCTION” OF DIHYDROFOLIC REDUCTASE ACTIVITY IN LEUKOCYTES AND ERYTHROCYTES OF PATIENTS TREATED WITH AMETHOPTERIN*

1963 • 92 citations

Intrinsic Resistance to Methotrexate of Cultured Mammalian Cells in Relation to the Inhibition Kinetics of Their Dihydrofolate Reductases

1976 • 91 citations

Studies on Dihydrofolic Reductase

1966 • 89 citations

Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts.

1979 • 89 citations

2,4-Diamino-5-methyl-6-[(3,4,5-trimethoxyanilino)methyl]quinazoline (TMQ), a potent non-classical folate antagonist inhibitor—I

1979 • 81 citations

Reversal of methotrexate binding to dihydrofolate reductase by dihydrofolate. Studies with pure enzyme and computer modeling using network thermodynamics.

1979 • 78 citations

A rapid radioassay for folic acid reductase and amethopterin

1966 • 76 citations

Dihydrofolate Reductase from the L1210R Murine Lymphoma. Fluorometric Measurements of the Interaction of the Enzyme with Coenzymes, Substrates, and Inhibitors^*

1966 • 76 citations

Effect of a single amino acid substitution on Escherichia coli dihydrofolate reductase catalysis and ligand binding.

1981 • 74 citations

Purification, Properties, and Synthesis of Dihydrofolate Reductase from Wild Type and Methotrexate-resistant Hamster Cells

1972 • 72 citations

A methotrexate insensitive variant of folate reductase present in two lines of methotrexate-resistant L5178Y cells

1980 • 61 citations

Characterization of chicken liver dihydrofolate reductase after purification by affinity chromatography and isoelectric focusing

1977 • 55 citations

Drug response, dihydrofolate reductase, and cytogenetics of amethopterin-resistant Chinese hamster cells in vitro.

1972 • 52 citations

Affinity chromatography of dihydrofolate reductase

1971 • 51 citations

Isolation and Characterization of the Multiple Forms of Dihydrofolate Reductase from Methotrexate-resistant Hamster Cells

1974 • 49 citations

Altered regulation of the rate of synthesis of dihydrofolate reductase in methotrexate-resistant hamster cells.

1976 • 49 citations

Porcine liver dihydrofolate reductase. Purification, properties, and amino acid sequence.

1979 • 48 citations

"Induction" of dihydrofolate reductase: purification and properties of the "induced" human erythrocyte and leukocyte enzyme and normal bone marrow enzyme.

1970 • 43 citations

ACTIVE-SITE DIRECTED IRREVERSIBLE INHIBITORS OF DIHYDROFOLATE REDUCTASE

1971 • 42 citations

Genetic characterization of methotrexate-resistant chinese hamster ovary cells

1976 • 41 citations

Purification and properties of dihydrofolate reductase from methotrexate-sensitive and methotrexate-resistant Chinese hamster ovary cells

1977 • 37 citations

A rapid radiometric assay for dihydrofolate reductase

1978 • 36 citations

Multiple enzyme forms from protein-bromphenol blue interaction during gel electrophoresis

1972 • 33 citations

TPNH-dependent binding of amethopterin by dihydrofolate reductase from Lactobacillus casei

1972 • 26 citations

Dihydrofolate Reductase from Bovine Liver

1975 • 26 citations

Comparison of folate reductases of sarcoma 180 cells, sensitive and resistant to amethopterin.

1967 • 24 citations

Purification and Properties of Folate Reductase, the Cellular Receptor of Amethopterin

1966 • 24 citations

Studies on the interaction of tritium-labeled aminopterin with dihydrofolic reductase

1963 • 22 citations

Dihydrofolate Reductase from the L1210 R Murine Lymphoma

1967 • 14 citations