Synthetic DNA delivery systems

A new technique for the assay of infectivity of human adenovirus 5 DNA

1973 • 9,740 citations

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

1995 • 6,103 citations

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

1987 • 4,937 citations

Direct Gene Transfer into Mouse Muscle in Vivo

1990 • 3,985 citations

Gene transfer into mouse lyoma cells by electroporation in high electric fields.

1982 • 2,413 citations

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

1999 • 1,702 citations

Polyamidoamine cascade polymers mediate efficient transfection of cells in culture

1993 • 1,186 citations

DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations.

1993 • 1,122 citations

Transfer of Genes to Humans: Early Lessons and Obstacles to Success

1995 • 1,023 citations

Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers.

1996 • 995 citations

Gene transfer into muscle by electroporation in vivo

1998 • 943 citations

High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNA

1999 • 934 citations

Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery

1999 • 819 citations

Stepwise dismantling of adenovirus 2 during entry into cells

1993 • 810 citations

In Vitro Gene Delivery by Degraded Polyamidoamine Dendrimers

1996 • 805 citations

Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells

1998 • 711 citations

DNA delivery from polymer matrices for tissue engineering

1999 • 669 citations

The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems.

1994 • 668 citations

Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle.

1992 • 657 citations

Immune responses to transgene–encoded proteins limit the stability of gene expression after injection of replication–defective adenovirus vectors

1996 • 640 citations

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

1990 • 603 citations

The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells

1998 • 568 citations

DNA-polycation nanospheres as non-viral gene delivery vehicles

1998 • 536 citations

Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery

2000 • 515 citations

Polylysine-based transfection systems utilizing receptor-mediated delivery

1998 • 506 citations

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.

1992 • 483 citations

Water-Soluble Polyion Complex Associates of DNA and Poly(ethylene glycol)−Poly(<scp>l</scp>-lysine) Block Copolymer

1997 • 463 citations

Protamine sulfate enhances lipid-mediated gene transfer

1997 • 448 citations

Key issues in non-viral gene delivery

1998 • 441 citations

Structure−Activity Relationships of Water-Soluble Cationic Methacrylate/Methacrylamide Polymers for Nonviral Gene Delivery

1999 • 402 citations

A peptide nucleic acid–nuclear localization signal fusion that mediates nuclear transport of DNA

1999 • 373 citations

Electric field mediated gene transfer

1982 • 364 citations

Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation

1999 • 324 citations

Targeting Genes: Delivery and Persistent Expression of a Foreign Gene Driven by Mammalian Regulatory Elements in Vivo

1989 • 292 citations

Encapsulation of plasmid DNA in biodegradable poly(d,l-lactic-co-glycolic acid) microspheres as a novel approach for immunogene delivery

1999 • 258 citations

Poly(?-lactide-co-glycolide)-encapsulated plasmid DNA elicits systemic and mucosal antibody responses to encoded protein after oral administration

1997 • 254 citations

PLGA microspheres containing plasmid DNA: Preservation of supercoiled DNA via cryopreparation and carbohydrate stabilization

1999 • 243 citations

Mannose Polyethylenimine Conjugates for Targeted DNA Delivery into Dendritic Cells

1999 • 243 citations

Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells

1999 • 234 citations

Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency

1999 • 218 citations

Infectious poliovirus RNA: a sensitive method of assay

1965 • 213 citations

From Genes to Gene Medicines: Recent Advances in Nonviral Gene Delivery

1998 • 205 citations

Novel pyridinium surfactants for efficient, nontoxic in vitro gene delivery

1997 • 203 citations

Loss of Binding and Entry of Liposome-DNA Complexes Decreases Transfection Efficiency in Differentiated Airway Epithelial Cells

1997 • 195 citations

Gene gun and other non-viral approaches for cancer gene therapy

1995 • 179 citations

Comparison of DNA-lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo.

1997 • 175 citations

The role of endosome destabilizing activity in the gene transfer process mediated by cationic lipids

1997 • 163 citations

Controlled Gene Delivery by DNA–Gelatin Nanospheres

1998 • 162 citations

A low rate of cell proliferation and reduced DNA uptake limit cationic lipid-mediated gene transfer to primary cultures of ciliated human airway epithelia

1997 • 159 citations

Lipidic Vector Systems for Gene Transfer

1997 • 155 citations

Controlled DNA delivery systems.

1999 • 155 citations

Major limitations in the use of cationic liposomes for DNA delivery

1998 • 151 citations

Gene gun delivery of mRNA in situ results in efficient transgene expression and genetic immunization.

1996 • 150 citations

Optimization of Cell Surface Binding Enhances Efficiency and Specificity of Molecular Conjugate Gene Delivery

1998 • 143 citations

Optimization of delivery of foreign DNA into higher-plant chloroplasts

1990 • 138 citations

Particulate systems and polymers for in vitro and in vivo delivery of polynucleotides

1990 • 137 citations

Poly(ethylene glycol)-block-poly(<scp>l</scp>-lysine) Dendrimer: Novel Linear Polymer/Dendrimer Block Copolymer Forming a Spherical Water-Soluble Polyionic Complex with DNA

1998 • 132 citations

Key issues in non-viral gene delivery: Adv Drug Deliv Rev

1998 • 131 citations

Nonviral Vectors for In Vivo Gene Delivery: Physicochemical and Pharmacokinetic Considerations

1997 • 129 citations

Pressure-mediated oligonucleotide transfection of rat and human cardiovascular tissues

1999 • 128 citations

New directions in liposome gene delivery

1999 • 123 citations

Targeted gene transfer to corneal endothelium in vivo by electric pulse

1998 • 115 citations

Synthetic peptide-based DNA complexes for nonviral gene delivery

1998 • 103 citations

Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo

1999 • 102 citations

Lipid fusion in oligonucleotide and gene delivery with cationic lipids

1998 • 101 citations

A Physicochemical Approach for Predicting the Effectiveness of Peptide-Based Gene Delivery Systems for Use in Plasmid-Based Gene Therapy

1998 • 96 citations

Assessing the potential of skin electroporation for the delivery of protein- and gene-based drugs

1998 • 92 citations

A new non-viral DNA delivery vector: the terplex system

1998 • 92 citations

Lipid-based systems for the intracellular delivery of genetic drugs

1999 • 90 citations

A Modular DNA Carrier Protein Based on the Structure of Diphtheria Toxin Mediates Target Cell-specific Gene Delivery

1998 • 89 citations

Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium

1999 • 79 citations

Toward development of a non-viral gene therapeutic

1997 • 76 citations

Liver‐directed gene transfer: a linear polyethylenimine derivative mediates highly efficient DNA delivery to primary hepatocytes in vitro and in vivo

1998 • 72 citations

The contribution of poly-L-lysine, epidermal growth factor and streptavidin to EGF/PLL/DNA polyplex formation

1998 • 57 citations

Enhanced reporter gene expression in cells transfected in the presence of DMI-2, an acid nuclease inhibitor

1998 • 53 citations

Gene Transfer Using a Novel Fusion Protein, GAL4/Invasin

1997 • 45 citations

Intracellular compartmentalization of DNA fragments in cultured airway epithelial cells mediated by cationic lipids.

1999 • 31 citations

Ultrasonic nebulization of cationic lipid-based gene delivery systems for airway administration.

1998 • 29 citations

Liposomes: from membrane models to gene therapy

1998 • 18 citations