Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information

1981 • 3,596 citations

Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion

1972 • 1,932 citations

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

Structure of the cro repressor from bacteriophage λ and its interaction with DNA

1981 • 545 citations

The operator-binding domain of λ repressor: structure and DNA recognition

1982 • 528 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

Structure of the DNA-Eco RI Endonuclease Recognition Complex at 3 Å Resolution

1986 • 492 citations

Structure of the represser–operator complex of bacteriophage 434

1987 • 365 citations

The three-dimensional structure of trp repressor

1985 • 342 citations

EVIDENCE FROM PHYSICAL CHEMISTRY REGARDING THE SIZE AND SHAPE OF PROTEIN MOLECULES FROM ULTRA‐CENTRIFUGATION, DIFFUSION, VISCOSITY, DIELECTRIC DISPERSION, AND DOUBLE REFRACTION OF FLOW

1941 • 295 citations

EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.

1976 • 259 citations

Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda promoter

1979 • 220 citations

Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of bacteriophage lambda.

1982 • 210 citations

Nucleotide Sequence Specificity of Restriction Endonucleases

1979 • 199 citations

Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

1981 • 194 citations

DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

1981 • 167 citations

The interaction of the recognition helix of lac repressor with lac operator.

1987 • 151 citations

Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors

1982 • 73 citations

The Interaction of the EcoRI Restriction Endonuclease with its Substrate

1980 • 61 citations

Positive-selection vectors utilizing lethality of the EcoRI endonuclease

1986 • 54 citations

Positive-selection cloning vehicle useful for overproduction of hybrid proteins

1983 • 54 citations

Site directed mutagenesis experiments suggest that Glu lll, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI

1986 • 47 citations

Clustering of null mutations in the EcoRI endonuclease

1987 • 40 citations

Analysis of secondary structures in M13mp8 (+) single‐stranded DNA by the pausing of DNA polymerase α

1985 • 33 citations

Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quenchflow apparatus: enzyme concentration-dependent activity change

1981 • 33 citations

Two Identical Subunits of the EcoRI Restriction Endonuclease Co‐operate in the Binding and Cleavage of the Palindromic Substrate

1982 • 16 citations

Prediction of secondary structure for Eco RI endonuclease.

1984 • 15 citations