Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins

1988 • 3,479 citations

A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins

1989 • 2,706 citations

Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

1985 • 2,505 citations

Current protocols in molecular biology

1988 • 2,473 citations

Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.

1985 • 2,450 citations

PROTEIN-DNA RECOGNITION

1984 • 1,649 citations

Scissors-Grip Model for DNA Recognition by a Family of Leucine Zipper Proteins

1989 • 996 citations

Crystal structure of trp represser/operator complex at atomic resolution

1988 • 899 citations

Zinc fingers: Gilt by association

1988 • 799 citations

Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonuclease

1975 • 798 citations

Dissecting the catalytic triad of a serine protease

1988 • 621 citations

Enzyme Structure and Mechanism

1978 • 573 citations

DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.

1980 • 534 citations

Base sequence and helix structure variation in B and A DNA

1983 • 522 citations

Gene amplification and analysis

1986 • 517 citations

Structure of the DNA-Eco RI Endonuclease Recognition Complex at 3 Å Resolution

1986 • 492 citations

Restriction Endonuclease

1976 • 405 citations

Role of the hydrophobic effect in stability of site-specific protein-DNA complexes

1989 • 380 citations

DNA Nucleotide Sequence Restricted by the RI Endonuclease

1972 • 365 citations

Specificity of substrate recognition by the EcoRI restriction endonuclease.

1975 • 313 citations

Kinked DNA in crystalline complex with EcoRI endonuclease

1984 • 303 citations

Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitro

1987 • 267 citations

Probing Steric and Hydrophobic Effects on Enzyme-Substrate Interactions by Protein Engineering

1986 • 240 citations

A genetic system for analysis of staphylococcal nuclease

1983 • 218 citations

Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

1981 • 194 citations

Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence.

1982 • 179 citations

Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis.

1985 • 172 citations

Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonuclease

1987 • 169 citations

Site-specific methylases induce the SOS DNA repair response in Escherichia coli

1987 • 169 citations

The DNA loop model for ara repression: AraC protein occupies the proposed loop sites in vivo and repression-negative mutations lie in these same sites.

1986 • 167 citations

DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

1981 • 167 citations

Altering the specificity of restriction endonuclease: effect of replacing Mg²⁺ with Mn²⁺

1978 • 135 citations

Nucleotide sequence of bacteriophage f1 DNA

1982 • 133 citations

The effects of base analogue substitutions on the cleavage by the EcoRI restriction endonuclease of octadeoxyribonucleotides containing modified EcoRI recognition sequences.

1986 • 131 citations

A new-specificity mutant of 434 repressor that defines an amino acid–base pair contact

1987 • 119 citations

Sites of allosteric shift in the structure of the cyclic AMP receptor protein

1985 • 114 citations

The negative charge of Glu-111 is required to activate the cleavage center of EcoRI endonuclease

1989 • 114 citations

The nucleotide sequence of pACYC177

1988 • 105 citations

Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences

1990 • 105 citations

Glu-111 is required for activation of the DNA cleavage center of EcoRI endonuclease

1989 • 104 citations

Coordinate ion pair formation between EcoRI endonuclease and DNA.

1983 • 102 citations

Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.

1989 • 92 citations

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymic activity, not, however, the preference of this restriction endonuclease for cleavage within the site -GAATTC-

1989 • 76 citations

DNA site recognition and reduced specificity of the Eco RI endonuclease.

1980 • 76 citations

Alternative DNA loops regulate the arabinose operon in Escherichia coli.

1988 • 75 citations

The EcoRI restriction endonuclease with bacteriophage λ DNA. Equilibrium binding studies

1980 • 71 citations

Alteration of the specificity of restriction endonucle, ases in the presence of organic solvents

1980 • 71 citations

Isolation and analysis of arc repressor mutants: Evidence for an unusual mechanism of DNA binding

1986 • 69 citations

The enfolding arms of EcoRI endonuclease: Role in DNA binding and cleavage

1986 • 68 citations

Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases

1989 • 67 citations

DOMINANT MUTATORS IN ESCHERICHIA COLI

1982 • 65 citations

Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strain.

1984 • 64 citations

DNA curvature in native and modified EcoRI recognition sites and possible influence upon the endonuclease cleavage reaction

1988 • 55 citations

Substrate recognition by the EcoRI endonuclease

1990 • 55 citations

Positive-selection cloning vehicle useful for overproduction of hybrid proteins

1983 • 54 citations

"Ultraviolet footprinting" accurately maps sequence-specific contacts and DNA kinking in the EcoRI endonuclease-DNA complex.

1988 • 53 citations

EcoRI* Specificity and Hydrogen Bonding

1982 • 51 citations

Cloning and Sequencing of Restriction Fragments Generated byEcoRI*

1982 • 47 citations

Site directed mutagenesis experiments suggest that Glu lll, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI

1986 • 47 citations

Relaxation of recognition sequence of specific endonuclease HlndIII

1986 • 46 citations

Clustering of null mutations in the EcoRI endonuclease

1987 • 40 citations

Determinants of EcoRI endonuclease sequence discrimination.

1989 • 36 citations

Restriction endonucleaseRsrI fromRhodobacter sphaeroides, an isoschizomer ofEcoRI: purification and properties

1988 • 32 citations

Sequence-specific endonuclease BamHI: relaxation of sequence recognition.

1982 • 32 citations

Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants

1989 • 31 citations

DNA site recognition and overmethylation by the Eco RI methylase.

1980 • 28 citations

The TaqI ‘star’ reaction: strand preferences reveal hydrogen-bond donor and acceptor sites in canonical sequence recognition

1988 • 28 citations

Cassettes of the fl intergenic region

1989 • 21 citations

The ability of the restriction endonuclease EcoRI to digest hemi-methylated versus fully cytosine-methylated DNA of the herpes tk promoter region

1988 • 17 citations

Overmethylation of DNAs by the EcoRI methylase

1978 • 16 citations

Phage Trojan horses: a conditional expression system for lethal genes

1989 • 10 citations