Solubilization of Proteins in Two-Dimensional Electrophoresis

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

1977 • 5,640 citations

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

1984 • 3,959 citations

The hydrophobic effect

1980 • 1,922 citations

Theory for the folding and stability of globular proteins

1985 • 1,248 citations

Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins

1986 • 801 citations

Two-dimensional gel electrophoresis of membrane proteins

1976 • 702 citations

[63] Properties of detergents

1979 • 681 citations

Methods for increasing the resolution of two-dimensional protein electrophoresis

1988 • 650 citations

Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis

1998 • 499 citations

Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients

1997 • 484 citations

Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate

1971 • 438 citations

Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension

1994 • 367 citations

[1] Extraction of tissue lipids with a solvent of low toxicity

1981 • 364 citations

Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis

1998 • 342 citations

New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis

1998 • 334 citations

Advances in electrophoresis

1988 • 317 citations

Solubilization of proteins for electrophoretic analyses

1996 • 316 citations

Improved and simplified in‐gel sample application using reswelling of dry immobilized pH gradients

1997 • 309 citations

[10] Reductive cleavage of cystine disulfides with tributylphosphine

1977 • 249 citations

Improved protein solubility in two‐dimensional electrophoresis using tributyl phosphine as reducing agent

1998 • 241 citations

The Relationship of Structure to the Effectiveness of Denaturing Agents for Proteins

1963 • 203 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Analytical techniques for cell fractions

1979 • 177 citations

A fluorimetric assay for available lysine in proteins

1981 • 176 citations

Cyanate formation in solutions of urea

1971 • 159 citations

Some improvements in two-dimensional gel electrophoresis of proteins

1977 • 157 citations

[14] Preparation of Extracts from Plants

1990 • 155 citations

Preparative two‐dimensional gel electrophoresis of membrane proteins

1997 • 144 citations

Advanced Organic Chemistry

1995 • 143 citations

Two‐dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients

1997 • 138 citations

Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues

1984 • 118 citations

Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis

1985 • 115 citations

Electrophoresis and electrofocusing in detergent containing media: A discussion of basic concepts

1981 • 108 citations

High resolution two‐dimensional polyacrylamide gel electrophoresis. I. Methodological procedures

1983 • 108 citations

Two dimensional benzyldimethyl-n-hexadecylammonium chloride → sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: A high capacity high resolution technique for the purification of proteins from complex mixtures

1989 • 104 citations

Gel electrophoresis of giant proteins: Solubilization and silver‐staining of titin and nebulin from single muscle fiber segments

1993 • 102 citations

On the Structural Stability and Solvent Denaturation of Proteins

1970 • 97 citations

Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analyses

1989 • 89 citations

[19] Extraction of water-soluble enzymes and proteins from membranes

1971 • 78 citations

On the Structural Stability and Solvent Denaturation of Proteins

1970 • 74 citations

Solubilization of yeast plasma membranes and mitochondria by different types of non-denaturing detergents

1983 • 69 citations

Effect of 2-mercaptoethanol on pH gradients in isoelectric focusing

1982 • 68 citations

Resolution of basic cellular proteins including histone variants by two-dimensional gel electrophoresis: Evaluation of lysine to arginine ratios and phosphorylation

1980 • 67 citations

Amidosulfobetaines, a family of detergents with improved solubilization properties: Application for isoelectric focusing under denaturing conditions

1990 • 67 citations

Alkaline urea solubilization, two-dimensional electrophoresis and lectin staining of mammalian cell plasma membrane and plant seed proteins

1980 • 64 citations

Electrophoresis in gels containing zwitterionic groups

1981 • 62 citations

[18] Purification of integral membrane proteins

1984 • 61 citations

Photopolymerization of polyacrylamide gels with methylene blue

1993 • 58 citations

Method for extraction of proteins from green plant tissues for two-dimensional polyacrylamide gel electrophoresis

1985 • 50 citations

On the Structural Stability and Solvent Denaturation of Proteins

1971 • 48 citations

An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.

1984 • 46 citations

An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.

1984 • 46 citations

Use of sodium dodecyl sulfate, alone, to separate chromatin proteins from deoxyribonucleoprotein of Arbacia punctulata sperm chromatin

1969 • 46 citations

Two-Dimensional Electrophoretic Mapping of Proteins of Bundle Sheath and Mesophyll Cells of the C₄ Grass Digitaria sanguinalis (L.) Scop. (Crabgrass)

1982 • 40 citations

Solubilization of plasma membranes in anionic, non-ionic and zwitterionic surfactants for iso-dalt analysis: a critical evaluation

1984 • 40 citations

A method for the two-dimensional electrophoresis of leaf proteins

1981 • 38 citations

Binding of Ampholine to transfer RNA

1976 • 37 citations

Fluctuations of non-histone chromosomal proteins in differentiating brain cortex and cerebellar neurons.

1980 • 37 citations

Sample preparation of human tumors prior to two‐dimensional electrophoresis of proteins

1995 • 37 citations

Effect of various detergents on protein migration in the second dimension of two-dimensional gels

1991 • 36 citations

[11] The design and synthesis of detergents for membrane biochemistry

1986 • 33 citations

A novel system for the two-dimensional electrophoresis of membrane proteins

1977 • 23 citations

[57] Two-dimensional electrophoresis in the analysis and preparation of cell organelle polypeptides

1987 • 22 citations

Two-dimensional electrophoresis of membrane proteins factors affecting resolution of rat-liver microsomal proteins

1983 • 22 citations

Effect of “stacking” on the resolving power of ultrathin-layer two-dimensional gel electrophoresis

1987 • 21 citations

Procedures for two-dimensional electrophoretic analysis of nuclear proteins

1986 • 18 citations

A reliable two‐dimensional gel electrophoresis procedure for separating neural proteins

1991 • 17 citations

Factors affecting the range of pH gradients in the isoelectric focusing dimennsion of two‐dimensional gel electrophoresis: The effects of reservoir electrolytes and loading procedures

1985 • 13 citations

Fractionation of chromatin nonhistone proteins

1973 • 12 citations

[41] Isolation of thylakod proteins

1980 • 12 citations

Protein degradation during different extraction procedures of malignant cells

1986 • 1 citations