Detection of Proteins on Two-Dimensional Electrophoresis Gels

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels

1974 • 8,693 citations

Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography

1975 • 4,693 citations

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

1987 • 4,186 citations

Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250

1988 • 2,665 citations

Difference gel electrophoresis. A single gel method for detecting changes in protein extracts

1997 • 2,181 citations

Fluorescence detection in automated DNA sequence analysis

1986 • 1,694 citations

Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining

1985 • 1,359 citations

Two new staining procedures for quantitative estimation of proteins on electrophoretic strips

1963 • 607 citations

Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis

1985 • 597 citations

Autoradiography using storage phosphor technology

1990 • 531 citations

Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels

1987 • 405 citations

A comparison between low background silver diammine and silver nitrate protein stains

1992 • 266 citations

Fluorographic detection of radioactivity in polyacrylamide gels with 2,5-diphenyloxazole in acetic acid and its comparison with existing procedures

1983 • 264 citations

SYPRO Orange and SYPRO Red Protein Gel Stains: One-Step Fluorescent Staining of Denaturing Gels for Detection of Nanogram Levels of Protein

1996 • 258 citations

Two-dimensional gel electrophoresis of proteins

1987 • 205 citations

Silver-staining of proteins in polyacrylamide gels: a general overview

2009 • 200 citations

Mechanisms of protein silver staining in polyacrylamide gels: A 10‐year synthesis

1990 • 192 citations

Mass Spectrometry of Whole Proteins Eluted from Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Gels

1997 • 187 citations

Coomassie blue-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for direct visualization of polypeptides during electrophoresis

1988 • 173 citations

Applications of SYPRO Orange and SYPRO Red Protein Gel Stains

1996 • 152 citations

Two‐dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients

1997 • 138 citations

Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels

1982 • 117 citations

Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins

1992 • 107 citations

Protein Reverse Staining: High-Efficiency Microanalysis of Unmodified Proteins Detected on Electrophoresis Gels

1995 • 97 citations

Rapid visuallzation of protein-dodecyl sulfate complexes in polyacrylamide gels

1976 • 96 citations

Sodium dodecyl sulfate-gel electrophoresis: Staining of polypeptides using heavy metal salts

1988 • 76 citations

Comparison of in‐gel and on‐membrane digestion methods at low to sub‐pmol level for subsequent peptide and fragment‐ion mass analysis using matrix‐assisted laser‐desorption/ionization mass spectrometry

1997 • 74 citations

Optical imaging of the spatial distribution of beta-particles emerging from surfaces.

1989 • 72 citations

'Catalysts' for polyacrylamide gel polymerization and detection of proteins by silver staining.

1988 • 60 citations

Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins Labeled with the Fluorophore Monobromobimane Prior to First-Dimensional Isoelectric Focusing: Imaging of the Fluorescent Protein Spot Patterns Using a Cooled Charge-Coupled Device

1993 • 59 citations

One-Step Coomassie Brilliant Blue R-250 Staining of Proteins in Polyacrylamide Gel

1993 • 47 citations

A Permanent Zn2+ Reverse Staining Method for the Detection and Quantification of Proteins in Polyacrylamide Gels

1993 • 47 citations

Silver staining of proteins: Standardized procedure for two-dimensional gels bound to polyester sheets

1986 • 46 citations

Rapid imaging, using a cooled charge‐coupled‐device, of fluorescent two‐dimensional polyacrylamide gels produced by labelling proteins in the first‐dimensional isoelectric focusing gel with the fluorophore 2‐methoxy‐2,4‐diphenyl‐3(2H)furanone

1988 • 44 citations

Detection of Polypeptides in Two-Dimensional Gels Using Silver Staining

1984 • 43 citations

The use of a new reactive dye for quantitation of prestained proteins on polyacrylamide gels

1977 • 35 citations

Fluorescamine staining of nonhistone chromatin proteins as revealed by two-dimensional polyacrylamide gel electrophoresis

1980 • 32 citations

Ion-enhanced fluorescence staining of sodium dodecyl sulfate-polyacrylamide gels using bis(8-p-toluidino-1-naphthalenesulfonate)

1987 • 30 citations

A multiple high-resolution mini two-dimensional polyacrylamide gel electrophoresis system: Imaging two-dimensional gels using a cooled charge-coupled device after staining with silver or labeling with fluorophore

1991 • 28 citations

Detection of Five Nanograms of Protein by Two-Minute Nile Red Staining of Unfixed SDS Gels

1996 • 28 citations

Protein analysis on two-dimensional polyacrylamide gels in the femtogram range: Use of a new sulfur-labeling reagent

1988 • 27 citations

Silver staining of histones in Triton-acid-urea gels

1983 • 24 citations

Transfer of silver-stained proteins from polyacrylamide gels to polyvinylidene difluoride membranes

1991 • 21 citations

Rapid fluorescent staining of histones in sodium dodecyl sulfate-polyacrylamide gels

1984 • 19 citations

Procedures for two-dimensional electrophoretic analysis of nuclear proteins

1986 • 18 citations

Localization and quantitation of tritiated compounds in tissue sections with a gaseous detector of beta particles: comparison with film autoradiography.

1991 • 17 citations

Zinc‐imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity

1996 • 14 citations

Detection and recovery of proteins from gels following zinc chloride staining.

1990 • 10 citations

Suitability of sulfur labelling reagent (Amersham) as an in vitro protein radiolabelling reagent for two‐dimensional electrophoresis

1986 • 4 citations