Molecular Cloning, Expression, and Characterization of the Human Mitogen-Activated Protein Kinase p44^erk1

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs

1987 • 5,139 citations

The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains

1988 • 5,115 citations

Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid Proteins

1981 • 2,972 citations

ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF

1991 • 1,901 citations

An Osmosensing Signal Transduction Pathway in Yeast

1993 • 1,252 citations

Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).

1991 • 1,041 citations

The Primary Structure of MEK, a Protein Kinase that Phosphorylates the ERK Gene Product

1992 • 931 citations

Synthetic oligonucleotide probes deduced from amino acid sequence data

1985 • 759 citations

An Insulin-Stimulated Protein Kinase Similar to Yeast Kinases Involved in Cell Cycle Control

1990 • 701 citations

Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.

1987 • 604 citations

Extracellular signal-regulated kinases: ERKs in progress.

1991 • 519 citations

Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro.

1993 • 503 citations

Recent progress in characterization of protein kinase cascades for phosphorylation of ribosomal protein S6

1991 • 442 citations

Mitogen-activated protein kinases: versatile transducers for cell signaling

1992 • 439 citations

FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation

1990 • 437 citations

In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase

1991 • 422 citations

Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase

1991 • 407 citations

[39] Unified approach to alignment and phylogenies

1990 • 398 citations

Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.

1991 • 392 citations

A yeast mitogen-activated protein kinase homolog (Mpk1p) mediates signalling by protein kinase C.

1993 • 373 citations

Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A431 cells.

1992 • 370 citations

Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.

1991 • 369 citations

Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.

1991 • 344 citations

MAP kinase by any other name smells just as sweet

1992 • 341 citations

Molecular cloning, characterization, and expression of a cDNA encoding the "80- to 87-kDa" myristoylated alanine-rich C kinase substrate: a major cellular substrate for protein kinase C.

1989 • 318 citations

Requirements for Phosphorylation of Map Kinase During Meiosis in Xenopus Oocytes

1992 • 316 citations

A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae

1989 • 298 citations

Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.

1992 • 292 citations

Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.

1991 • 274 citations

Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.

1992 • 264 citations

Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.

1988 • 257 citations

MAP kinase activator from insulin-stimulated skeletal muscle is a protein threonine/tyrosine kinase.

1992 • 256 citations

pp54 microtubule-associated protein 2 kinase. A novel serine/threonine protein kinase regulated by phosphorylation and stimulated by poly-L-lysine.

1990 • 256 citations

Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.

1988 • 240 citations

Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product.

1992 • 236 citations

Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation.

1992 • 232 citations

Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases.

1992 • 202 citations

MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro

1993 • 190 citations

Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.

1986 • 189 citations

cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.

1993 • 179 citations

Purification and properties of extracellular regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase

1991 • 175 citations

Molecular structure of a protein-tyrosine/threonine kinase activating p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase.

1993 • 171 citations

Ordered phosphorylation of p42^mapk by MAP kinase kinase

1992 • 159 citations

Functional expression and growth factor activation of an epitope-tagged p44 mitogen-activated protein kinase, p44mapk.

1992 • 156 citations

Heterogeneous expression of four MAP kinase isoforms in human tissues

1992 • 143 citations

Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.

1991 • 143 citations

Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.

1990 • 141 citations

Tyrosyl Phosphorylation and Activation of MAP Kinases by p56^lck

1992 • 140 citations

Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine.

1991 • 113 citations

The phorbol ester-dependent activator of the mitogen-activated protein kinase p42mapk is a kinase with specificity for the threonine and tyrosine regulatory sites.

1992 • 111 citations

Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization

1988 • 110 citations

Phorbol ester stimulates a protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product.

1992 • 109 citations

Sequence of pp42/MAP kinase, a serine/threonine kinase regulated by tyrosine phosphorylation

1991 • 96 citations

Immunological characterization of avian MAP kinases: evidence for nuclear localization.

1992 • 89 citations

Molecular cloning and characterization of the acidic 80-kDa protein kinase C substrate from rat brain. Identification as a glycoprotein.

1991 • 78 citations

Extracellular signal-regulated kinases 2 autophosphorylates on a subset of peptides phosphorylated in intact cells in response to insulin and nerve growth factor: analysis by peptide mapping.

1992 • 71 citations

Extracellular signal-regulated kinases in T cells: Characterization of human ERK1 and ERK2 cDNAs

1992 • 67 citations

Role of protein phosphorylation in the maturation-induced activation of a myelin basic protein kinase from sea star oocytes.

1991 • 65 citations

Identification of Tyr-185 as the site of tyrosine autophosphorylation of recombinant mitogen-activated protein kinase p42mapk.

1992 • 58 citations

Recombinant lecithin:cholesterol acyltransferase containing a Thr123–>Ile mutation esterifies cholesterol in low density lipoprotein but not in high density lipoprotein.

1993 • 49 citations

95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site.

1992 • 46 citations

A mitogen-activated protein (MAP) kinase activating factor in mammalian mitogen-stimulated cells is homologous to Xenopus M phase MAP kinase activator.

1992 • 45 citations

Identification of epidermal growth factor Thr-669 phosphorylation site peptide kinases as distinct MAP kinases and p34cdc2

1992 • 32 citations

Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation

1991 • 29 citations

Map kinases—ubiquitous signal transducers and potentially important components of the cell cycling machinery in eukaryotes

1992 • 25 citations

Sequence of a rat cDNA encoding the ERK1-MAP kinase

1992 • 19 citations