Elements Upstream of the AAUAAA within the Human Immunodeficiency Virus Polyadenylation Signal Are Required for Efficient Polyadenylation In Vitro

Current protocols in molecular biology

1988 • 7,584 citations

3′ Non-coding region sequences in eukaryotic messenger RNA

1976 • 3,001 citations

The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs

1981 • 819 citations

The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3′ termini

1985 • 652 citations

Role of the Conserved AAUAAA Sequence: Four AAUAAA Point Mutants Prevent Messenger RNA 3′ End Formation

1984 • 561 citations

Accurate cleavage and polyadenylation of exogenous RNA substrate

1985 • 334 citations

Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA

1983 • 278 citations

Requirement of a downstream sequence for generation of a poly(A) addition site

1984 • 261 citations

A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formation

1984 • 251 citations

Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit β-globin mRNA 3′ end formation

1987 • 229 citations

Site-specific polyadenylation in a cell-free reaction

1984 • 223 citations

Efficiency of Utilization of the Simian Virus 40 Late Polyadenylation Site: Effects of Upstream Sequences

1989 • 205 citations

Sequences capable of restoring poly(A) site function define two distinct downstream elements.

1986 • 175 citations

Sequences 5' to the polyadenylation signal mediate differential poly(A) site use in hepatitis B viruses.

1990 • 159 citations

Poly(A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences

1985 • 152 citations

A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes.

1985 • 139 citations

The human immunodeficiency virus type 1 polyadenylylation signal: a 3' long terminal repeat element upstream of the AAUAAA necessary for efficient polyadenylylation.

1991 • 136 citations

Relative position and strengths of poly(A) sites as well as transcription termination are critical to membrane versus secreted mu-chain expression during B-cell development.

1987 • 110 citations

Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse β-major globin terminator element

1985 • 109 citations

A Uridylate Tract Mediates Efficient Heterogeneous Nuclear Ribonucleoprotein C Protein-RNA Cross-Linking and Functionally Substitutes for the Downstream Element of the Polyadenylation Signal

1990 • 107 citations

A dissection of the cauliflower mosaic virus polyadenylation signal.

1991 • 105 citations

Linker-scanning mutational analysis of the transcriptional activity of the human immunodeficiency virus type 1 long terminal repeat

1991 • 104 citations

The HTLV-I rex response element mediates a novel form of mRNA polyadenylation

1991 • 101 citations

Efficient polyadenylation within the human immunodeficiency virus type 1 long terminal repeat requires flanking U3-specific sequences

1991 • 90 citations

Poly(A) site choice rather than splice site choice governs the regulated production of IgM heavy-chain RNAs.

1988 • 87 citations

Herpes simplex virus induces a processing factor that stimulates poly(A) site usage

1989 • 84 citations

Sequences on the 3′ Side of Hexanucleotide AAUAAA Affect Efficiency of Cleavage at the Polyadenylation Site

1984 • 72 citations

The AAUAAA Sequence Is Required both for Cleavage and for Polyadenylation of Simian Virus 40 Pre-mRNA In Vitro

1986 • 69 citations

Occlusion of the HIV poly(A) site.

1991 • 68 citations

Regulation of poly(A) site selection in adenovirus

1989 • 61 citations

Sequences Downstream of AAUAAA Signals Affect Pre-mRNA Cleavage and Polyadenylation In Vitro Both Directly and Indirectly

1989 • 61 citations

Identification of a Sequence Element on the 3′ Side of AAUAAA Which Is Necessary for Simian Virus 40 Late mRNA 3′-End Processing

1985 • 59 citations

The Regulated Production of μ_m and μ_s mRNA Is Dependent on the Relative Efficiencies of μ_s Poly(A) Site Usage and the Cμ4-to-M1 Splice

1989 • 57 citations

Identification of a U5-specific sequence required for efficient polyadenylation within the human immunodeficiency virus long terminal repeat

1989 • 52 citations

Definition of Essential Sequences and Functional Equivalence of Elements Downstream of the Adenovirus E2A and the Early Simian Virus 40 Polyadenylation Sites

1985 • 51 citations

The efficiency of RNA 3'-end formation is determined by the distance between the cap site and the poly(A) site in spleen necrosis virus.

1990 • 45 citations

Involvement of Long Terminal Repeat U3 Sequences Overlapping the Transcription Control Region in Human Immunodeficiency Virus Type 1 mRNA 3′ End Formation

1991 • 43 citations

Identification of Sequences in the Herpes Simplex Virus Thymidine Kinase Gene Required for Efficient Processing and Polyadenylation

1985 • 41 citations

An RNA secondary structure juxtaposes two remote genetic signals for human T-cell leukemia virus type I RNA 3'-end processing

1991 • 37 citations

ATTAAA As Well As Downstream Sequences Are Required for RNA 3′-End Formation in the E3 Complex Transcription Unit of Adenovirus

1985 • 35 citations

Spatial constraints on polyadenylation signal function.

1990 • 30 citations

Identification of a Complex Associated with Processing and Polyadenylation In Vitro of Herpes Simplex Virus Type 1 Thymidine Kinase Precursor RNA

1987 • 26 citations

Fine-Structure Analysis of the Processing and Polyadenylation Region of the Herpes Simplex Virus Type 1 Thymidine Kinase Gene by Using Linker Scanning, Internal Deletion, and Insertion Mutations

1986 • 20 citations

Preparation of mammalian extracts active in polyadenylation

1990 • 15 citations

In Vitro Cleavage of the Simian Virus 40 Early Polyadenylation Site Adjacent to a Required Downstream TG Sequence

1986 • 8 citations