Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3‘-5‘ exonuclease activity.

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Structural basis for the 3′-5′ exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.

1991 • 1,059 citations

An attempt to unify the structure of polymerases

1990 • 640 citations

FUNCTION AND STRUCTURE RELATIONSHIPS IN DNA POLYMERASES

1994 • 611 citations

Compilation, alignment, and phylogenetic relationships of DNA polymerases

1993 • 590 citations

Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant

1991 • 495 citations

Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA

1993 • 473 citations

A conserved 3′→5′ exonuclease active site in prokaryotic and eukaryotic DNA polymerases

1989 • 433 citations

Enzymatic Synthesis of Deoxyribonucleic Acid

1968 • 373 citations

Proteolytic enzymes; a practical approach

1990 • 364 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

Compilation and alignment of DNA polymerase sequences

1991 • 339 citations

Selective Elimination of the Exonuclease Activity of the Deoxyribonucleic Acid Polymerase from Escherichia coli B by Limited Proteolysis

1970 • 326 citations

Cocrystal structure of an editing complex of Klenow fragment with DNA.

1988 • 324 citations

The 3′-5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.

1991 • 279 citations

Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity.

1991 • 277 citations

Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.

1990 • 261 citations

A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme.

1984 • 246 citations

Human DNA polymerase α: predicted functional domains and relationships with viral DNA polymerases

1989 • 201 citations

Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.

1992 • 194 citations

2.3 Å crystal structure of the catalytic domain of DNA polymerase β

1994 • 168 citations

Studies of the domain structure of mammalian DNA polymerase beta. Identification of a discrete template binding domain.

1990 • 165 citations

Primary structure of T4 DNA polymerase. Evolutionary relatedness to eucaryotic and other procaryotic DNA polymerases.

1988 • 134 citations

Proofreading by DNA polymerase III of Escherichia coli depends on cooperative interaction of the polymerase and exonuclease subunits.

1987 • 132 citations

How DNA Travels between the Separate Polymerase and 3′-5′-Exonuclease Sites of DNA Polymerase I (Klenow Fragment)

1989 • 129 citations

Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.

1993 • 109 citations

DNA substrate structural requirements for the exonuclease and polymerase activities of prokaryotic and phage DNA polymerases

1989 • 101 citations

Structure and function of the bacteriophage T4 DNA polymerase holoenzyme

1992 • 96 citations

The highly conserved amino acid sequence motif Tyr-Gly-Asp-Thr-Asp-Ser in alpha-like DNA polymerases is required by phage phi 29 DNA polymerase for protein-primed initiation and polymerization.

1990 • 92 citations

A domain of the klenow fragment of Escherichia coli DNA polymerase I has polymerase but no exonuclease activity

1986 • 90 citations

Processive proofreading is intrinsic to T4 DNA polymerase.

1992 • 87 citations

Nuclease Activity in a Fragment of Bacteriophage T4 Deoxyribonucleic Acid Polymerase Induced by the Amber Mutant am B22

1971 • 79 citations

The minimal kinetic mechanism for misincorporation by DNA polymerase I (Klenow fragment)

1992 • 76 citations

Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity.

1993 • 69 citations

Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid

1993 • 49 citations

Polymerization Activity of an α-Like DNA Polymerase Requires a Conserved 3′-5′ Exonuclease Active Site

1991 • 48 citations

Cloning and expression of T4 DNA polymerase.

1987 • 42 citations

Use of suppressor analysis to identify DNA polymerase mutations in herpes simplex virus which affect deoxynucleoside triphosphate substrate specificity

1992 • 23 citations

Site-specific mutagenesis of PRD1 DNA polymerase: Mutations in highly conserved regions of the family B DNA polymerase

1990 • 18 citations