Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Structural basis for the 3′-5′ exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.

1991 • 1,059 citations

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

1985 • 855 citations

Three-dimensional structure of the β subunit of E. coli DNA polymerase III holoenzyme: A sliding DNA clamp

1992 • 748 citations

An attempt to unify the structure of polymerases

1990 • 640 citations

Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant

1991 • 495 citations

A conserved 3′→5′ exonuclease active site in prokaryotic and eukaryotic DNA polymerases

1989 • 433 citations

Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases.

1988 • 405 citations

Replication factors required for SV40 DNA replication in vitro. I. DNA structure-specific recognition of a primer-template junction by eukaryotic DNA polymerases and their accessory proteins

1991 • 380 citations

Enzymatic Synthesis of Deoxyribonucleic Acid

1968 • 373 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

Studies on the Biochemical Basis of Spontaneous Mutation

1972 • 340 citations

Compilation and alignment of DNA polymerase sequences

1991 • 339 citations

The 3′-5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.

1991 • 279 citations

Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity.

1991 • 277 citations

Functions of replication factor C and proliferating-cell nuclear antigen: functional similarity of DNA polymerase accessory proteins from human cells and bacteriophage T4.

1990 • 261 citations

Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis

1989 • 258 citations

Studies on the activator 1 protein complex, an accessory factor for proliferating cell nuclear antigen-dependent DNA polymerase delta.

1991 • 254 citations

The 3′ to 5′ exonuclease activity located in the DNA polymerase delta subunit of Saccharomyces cerevisiae is required for accurate replication.

1991 • 230 citations

Kinetic characterization of the polymerase and exonuclease activities of the gene 43 protein of bacteriophage T4

1992 • 207 citations

Reconstitution of simian virus 40 DNA replication with purified proteins.

1990 • 158 citations

[61] Rapid kinetic analysis of mechanochemical adenosinetriphosphatases

1986 • 151 citations

Primary structure of T4 DNA polymerase. Evolutionary relatedness to eucaryotic and other procaryotic DNA polymerases.

1988 • 134 citations

Analysis of the ATPase subassembly which initiates processive DNA synthesis by DNA polymerase III holoenzyme.

1991 • 99 citations

Protein‐protein interactions at a DNA replication fork: bacteriophage T4 as a model

1992 • 93 citations

Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 antimutator DNA polymerase is defective in strand displacement.

1976 • 84 citations

Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants

1988 • 84 citations

Prokaryotic DNA replication mechanisms

1987 • 82 citations

Mechanism of DNA replication fidelity for three mutants of DNA polymerase I: Klenow fragment KF(exo+), KF(polA5), and KF(exo-)

1991 • 81 citations

Nuclease Activity in a Fragment of Bacteriophage T4 Deoxyribonucleic Acid Polymerase Induced by the Amber Mutant am B22

1971 • 79 citations

DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies.

1991 • 69 citations

Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells.

1992 • 66 citations

Cloning and expression of T4 DNA polymerase.

1987 • 42 citations

Structure-function studies of the bacteriophage T4 DNA polymerase

1985 • 11 citations