Nuclear Magnetic Resonance Methods for Quantifying Microsecond-to-Millisecond Motions in Biological Macromolecules

Effects of Diffusion on Free Precession in Nuclear Magnetic Resonance Experiments

1954 • 6,078 citations

Modified Spin-Echo Method for Measuring Nuclear Relaxation Times

1958 • 5,632 citations

Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity

1992 • 2,231 citations

Protein NMR Spectroscopy: Principles and Practice

1995 • 1,642 citations

Encyclopedia of nuclear magnetic resonance

2003 • 1,638 citations

Reaction Rates by Nuclear Magnetic Resonance

1958 • 1,561 citations

Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins

1989 • 1,395 citations

Dynamics of Proteins and Nucleic Acids

1988 • 1,274 citations

NMR-Relaxation Mechanisms of O17 in Aqueous Solutions of Paramagnetic Cations and the Lifetime of Water Molecules in the First Coordination Sphere

1962 • 1,264 citations

Dynamic NMR Spectroscopy

1983 • 1,111 citations

Computer-optimized decoupling scheme for wideband applications and low-level operation

1985 • 1,104 citations

Protein Folding Intermediates: Native-State Hydrogen Exchange

1995 • 980 citations

Protein NMR Spectroscopy. Principles and Practice

1997 • 974 citations

Backbone Dynamics of Ribonuclease HI: Correlations with Structure and Function in an Active Enzyme

1995 • 965 citations

Sensitivity improvement in proton-detected two-dimensional heteronuclear correlation NMR spectroscopy

1991 • 931 citations

The importance of not saturating water in protein NMR. Application to sensitivity enhancement and NOE measurements

1993 • 891 citations

How do small single-domain proteins fold?

1998 • 876 citations

Dynamic NMR spectroscopy

1982 • 707 citations

Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins

1993 • 692 citations

A Relaxation-Compensated Carr−Purcell−Meiboom−Gill Sequence for Characterizing Chemical Exchange by NMR Spectroscopy

1999 • 648 citations

Rotational diffusion anisotropy of human ubiquitin from 15N NMR relaxation

1995 • 528 citations

Nuclear Magnetic Resonance Study of the Protolysis of Trimethylammonium Ion in Aqueous Solution—Order of the Reaction with Respect to Solvent

1963 • 527 citations

Comparison of different modes of two-dimensional reverse-correlation NMR for the study of proteins

1990 • 510 citations

Spectral density function mapping using 15N relaxation data exclusively

1995 • 506 citations

Nuclear Magnetic Resonance Study of the Proton Transfer in Water

1961 • 501 citations

A heteronuclear correlation experiment for simultaneous determination of 15N longitudinal decay and chemical exchange rates of systems in slow equilibrium

1994 • 430 citations

A general two-site solution for the chemical exchange produced dependence of T2 upon the carr-Purcell pulse separation

1972 • 384 citations

Interference effects in the relaxation of a pair of unlike nuclei

1984 • 376 citations

The Static Magnetic Field Dependence of Chemical Exchange Linebroadening Defines the NMR Chemical Shift Time Scale

2000 • 335 citations

Detection of rare partially folded molecules in equilibrium with the native conformation of RNaseH

1996 • 316 citations

Dynamical Mapping of E. coli Thioredoxin via ¹³C NMR Relaxation Analysis

1996 • 314 citations

Comparison of the Backbone Dynamics of a Folded and an Unfolded SH3 Domain Existing in Equilibrium in Aqueous Buffer

1995 • 299 citations

Selective Methyl Group Protonation of Perdeuterated Proteins

1996 • 299 citations

Redistribution and loss of side chain entropy upon formation of a calmodulin-peptide complex.

2000 • 298 citations

A general method for assigning NMR spectra of denatured proteins using 3D HC(CO)NH-TOCSY triple resonance experiments

1993 • 297 citations

Nuclear Magnetic Resonance Studies of Biopolymer Dynamics

1996 • 291 citations

NMR of Chemically Exchanging Systems

1980 • 278 citations

Direct Measurements of the Dissociation-Rate Constant for Inhibitor-Enzyme Complexes via the T1ρ and T2 (CPMG) Methods

1994 • 278 citations

Protein Backbone Dynamics and ¹⁵N Chemical Shift Anisotropy from Quantitative Measurement of Relaxation Interference Effects

1996 • 275 citations

Protein dynamics from NMR

1998 • 275 citations

Monitoring Macromolecular Motions on Microsecond to Millisecond Time Scales by R_1ρ−R₁ Constant Relaxation Time NMR Spectroscopy

1996 • 271 citations

Chemical Rate Processes and Magnetic Resonance

1965 • 265 citations

Flap opening and dimer-interface flexibility in the free and inhibitor-bound HIV protease, and their implications for function

1999 • 264 citations

Optimized recording of heteronuclear multidimensional NMR spectra using pulsed field gradients

1992 • 264 citations

Studies of chemical exchange by nuclear magnetic relaxation in the rotating frame

1970 • 260 citations

Suppression of the effects of cross-correlation between dipolar and anisotropic chemical shift relaxation mechanisms in the measurement of spin-spin relaxation rates

1992 • 255 citations

Longitudinal and Transverse ¹H−¹⁵N Dipolar/¹⁵N Chemical Shift Anisotropy Relaxation Interference: Unambiguous Determination of Rotational Diffusion Tensors and Chemical Exchange Effects in Biological Macromolecules

1998 • 250 citations

Probing molecular motion by NMR

1997 • 241 citations

A TROSY CPMG sequence for characterizing chemical exchange in large proteins.

1999 • 236 citations

Increased protein backbone conformational entropy upon hydrophobic ligand binding.

1999 • 235 citations

Motional model analyses of protein and peptide dynamics using 13C and 15N NMR relaxation

1997 • 232 citations

Temperature dependence of intramolecular dynamics of the basic leucine zipper of GCN4: implications for the entropy of association with DNA

1999 • 215 citations

A Test of the Model-Free Formulas. Effects of Anisotropic Rotational Diffusion and Dimerization

1994 • 195 citations

Millisecond-timescale motions contribute to the function of the bacterial response regulator protein Spo0F

1999 • 191 citations

Dynamics of Ribonuclease H: Temperature Dependence of Motions on Multiple Time Scales

1996 • 180 citations

Protein dynamics studied by rotating frame 15N spin relaxation times

1993 • 178 citations

Theories of chemical shift anisotropies in proteins and nucleic acids

1998 • 174 citations

Frequency Spectrum of NH Bonds in Eglin c from Spectral Density Mapping at Multiple Fields

1995 • 168 citations

NMR Experiments for the Measurement of Carbon Relaxation Properties in Highly Enriched, Uniformly 13C,15N-Labeled Proteins: Application to 13C.alpha. Carbons

1994 • 161 citations

Structural dynamics in the C-terminal domain of calmodulin at low calcium levels 1 1Edited by P. E. Wright

1999 • 155 citations

Using buried water molecules to explore the energy landscape of proteins

1996 • 153 citations

Protein NMR relaxation: theory, applications and outlook

1998 • 145 citations

Backbone dynamics of (1–71)bacterioopsin studied by two‐dimensional ¹H‐¹⁵N NMR spectroscopy

1994 • 140 citations

Untitled

1997 • 140 citations

Variability of the ¹⁵N Chemical Shift Anisotropy in Escherichia coli Ribonuclease H in Solution

1999 • 134 citations

Backbone dynamics and energetics of a Calmodulin domain mutant exchanging between closed and open conformations

1999 • 129 citations

An Off-resonance Rotating Frame Relaxation Experiment for the Investigation of Macromolecular Dynamics Using Adiabatic Rotations

1998 • 129 citations

Sensitivity Improvement of Transverse Relaxation-Optimized Spectroscopy

1999 • 124 citations

Using Amide ¹H and ¹⁵N Transverse Relaxation To Detect Millisecond Time-Scale Motions in Perdeuterated Proteins: Application to HIV-1 Protease

1998 • 119 citations

Relaxation times in systems with chemical exchange: Some exact solutions

1971 • 116 citations

Estimating the time scale of chemical exchange of proteins from measurements of transverse relaxation rates in solution.

1999 • 113 citations

2D Chemical exchange NMR spectroscopy by proton-detected heteronuclear correlation

1989 • 113 citations

Correlation between binding and dynamics at SH2 domain interfaces

1998 • 110 citations

Improved RNA Structure Determination by Detection of NOE Contacts to Exchange-Broadened Amino Protons

1995 • 107 citations

fd coat protein structure in membrane environments: structural dynamics of the loop between the hydrophobic trans-membrane helix and the amphipathic in-plane helix

1997 • 106 citations

Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain

1998 • 104 citations

Water¹H Magnetic Relaxation Dispersion in Protein Solutions. A Quantitative Assessment of Internal Hydration, Proton Exchange, and Cross-Relaxation

1997 • 104 citations

Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motions.

1997 • 93 citations

A Method for DeterminingB1Field Inhomogeneity. Are the Biases Assumed in Heteronuclear Relaxation Experiments Usually Underestimated?

1999 • 92 citations

Conformational backbone dynamics of the cyclic decapeptide antamanide. Application of a new multiconformational search algorithm based on NMR data

1993 • 88 citations

Transverse ¹³C Relaxation of CHD₂ Methyl Isotopmers To Detect Slow Conformational Changes of Protein Side Chains

1999 • 81 citations

Residence times of the buried water molecules in bovine pancreatic trypsin inhibitor and its G36S mutant

1995 • 78 citations

Minimizing the effects of radio-frequency heating in multidimensional NMR experiments

1993 • 77 citations

Dynamics of proteins and nucleic acids

1988 • 76 citations

Exchange of two-spin order in nuclear magnetic resonance: separation of exchange and cross-relaxation processes

1985 • 75 citations

Improved HSQC experiments for the observation of exchange broadened signals

1996 • 72 citations

Dynamic studies of a fibronectin type I module pair at three frequencies: Anisotropic modelling and direct determination of conformational exchange

1996 • 71 citations

Chemical exchange and nmr T2 relaxation—The multisite case

1978 • 70 citations

Study of dynamic processes in liquids using off-resonance rf irradiation

1999 • 70 citations

Microsecond time scale dynamics in the RXR DNA-binding domain from a combination of spin-echo and off-resonance rotating frame relaxation measurements.

1999 • 63 citations

Longitudinal two-spin order in 2D exchange spectroscopy (NOESY)

1984 • 62 citations

The Use of Residual Dipolar Coupling in Concert with Backbone Relaxation Rates to Identify Conformational Exchange by NMR

1999 • 60 citations

Studies of slow conformational equilibria in macromolecules by exchange of heteronuclear longitudinal 2-spin-order in a 2D difference correlation experiment

1991 • 57 citations

[14] Nuclear magnetic resonance line-shape analysis and determination of exchange rates

1989 • 52 citations

Correlation between changes in nuclear magnetic resonance order parameters and conformational entropy: Molecular dynamics simulations of native and denatured staphylococcal nuclease

2000 • 51 citations

Untitled

1999 • 47 citations

Study of protein dynamics in solution by measurement of 13C?-13CO NOE and 13CO longitudinal relaxation

1996 • 44 citations

Elimination of cross-relaxation effects from two-dimensional chemical-exchange spectra of macromolecules

1990 • 44 citations

NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.

1999 • 44 citations

¹⁵N R_1ρ Measurements Allow the Determination of Ultrafast Protein Folding Rates

2000 • 39 citations

Influence of Chemical Exchange among Homonuclear Spins in Heteronuclear Coherence-Transfer Experiments in Liquids

1995 • 37 citations

Characterization of millisecond time-scale dynamics in the molten globule state of α-lactalbumin by NMR

1999 • 36 citations

[3] Two-dimensional exchange spectroscopy of proteins

1994 • 35 citations

Brownian motion and its effects in NMR chemical exchange and relaxation in liquids

1996 • 31 citations

Measurement of Relaxation-Rate Constants Using Constant-Time Accordion NMR Spectroscopy

1994 • 28 citations

Use of ¹³C−¹³C NOE for the Assignment of NMR Lines of Larger Labeled Proteins at Larger Magnetic Fields

1996 • 27 citations

AV77 Hinge Mutation Stabilizes the Helix-Turn-Helix Domain of Repressor

1996 • 25 citations

Measurement of 13C?-13CO cross-relaxation rates in 15N-/13C-labelled proteins

1996 • 25 citations

Internal motion time scales of a small, highly stable and disulfide-rich protein: a 15N, 13C NMR and molecular dynamics study.

1999 • 23 citations

Slow conformational dynamics of an endonuclease persist in its complex with its natural protein inhibitor

2000 • 20 citations

The influence of DNA binding on the backbone dynamics of the yeast cell-cycle protein Mbp1.

2000 • 19 citations

Low-Frequency Molecular Dynamics Studied by Spin-Lock Field Cycling Imaging

2000 • 19 citations

Separating the contributions to 15N transverse relaxation in a fibronectin type III domain.

1999 • 11 citations

Density matrix theory for calculating magnetization transfer and dynamic lineshape effects

1995 • 11 citations

Direct determination of the heteronuclear T1/T2 ratio by off-resonance steady-state magnetization measurement: Investigation of the possible application to fast exchange characterization of 15N-labeled proteins.

1999 • 3 citations