High-level expression in Escherichia coli of calcium-binding domains of an embryonic sea urchin protein

Experiments in molecular genetics

1972 • 28,260 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322

1979 • 1,807 citations

Structure And Evolution Of Calcium-Modulated Protein

1980 • 942 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

Revised sequence of the tetracycline-resistance gene of pBR322

1983 • 287 citations

Interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate

1980 • 273 citations

Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda promoter

1979 • 220 citations

Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.

1982 • 189 citations

[35] Use of the λ phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles

1979 • 127 citations

Localization of a family of MRNAS in a single cell type and its precursors in sea urchin embryos.

1983 • 114 citations

Accumulation in embryogenesis of five mRNAs enriched in the ectoderm of the sea urchin pluteus

1981 • 114 citations

A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids

1981 • 109 citations

Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.

1983 • 108 citations

Differential expression of the actin gene family of Strongylocentrotus purpuratus

1984 • 107 citations

A plasmid cloning vehicle allowing a positive selection for inserted fragments

1980 • 105 citations

A single base-pair alteration is responsible for the DNA overproduction phenotype of a plasmid copy-number mutant

1981 • 99 citations

Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.

1983 • 93 citations

Novel proteins belonging to the troponin C superfamily are encoded by a set of mRNAs in sea urchin embryos

1984 • 74 citations

Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.

1982 • 70 citations

Positive-selection cloning vehicle useful for overproduction of hybrid proteins

1983 • 54 citations

A family of proteins accumulating in ectoderm of sea urchin embryos specified by two related cDNA clones

1982 • 45 citations

Regulated expression by readthrough translation from a plasmid-encoded beta-galactosidase

1978 • 37 citations

The RNA primer promoter, as defined in vitro, is essential for pMB1 plasmid replication in vivo

1982 • 16 citations