High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

Determinant of cistron specificity in bacterial ribosomes

1975 • 1,363 citations

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

1977 • 711 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

Structure and variation of human α1–antitrypsin

1982 • 616 citations

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

1981 • 423 citations

Cigarette Smoking Induces Functional Antiprotease Deficiency in the Lower Respiratory Tract of Humans

1979 • 398 citations

In vitro suppression of serum elastase-inhibitory capacity by reactive oxygen species generated by phagocytosing polymorphonuclear leukocytes.

1979 • 293 citations

α1-Antitrypsin deficiency detection by direct analysis of the mutation in the gene

1983 • 277 citations

Replacement therapy of alpha 1-antitrypsin deficiency. Reversal of protease-antiprotease imbalance within the alveolar structures of PiZ subjects.

1981 • 275 citations

Enzymatic inactivation of human alpha-1-proteinase inhibitor by neutrophil myeloperoxidase

1979 • 270 citations

Mouse macrophage elastase. Purification and characterization as a metalloproteinase

1981 • 260 citations

Purified λ regulatory protein cII positively activates promoters for lysogenic development

1981 • 241 citations

Nucleotide sequence of cro, cII and part of the O gene in phage λ DNA

1978 • 222 citations

Coliphage λnutL−: A unique class of mutants defective in the site of gene N product utilization for antitermination of leftward transcription

1978 • 207 citations

Cloning and sequence of cDNA coding for alpha 1-antitrypsin.

1981 • 176 citations

Sequence homology and structural comparison between the chromosomal human α1-antitrypsin and chicken ovalbumin genes

1982 • 128 citations

Characterization of α₁-Antitrypsin in the Inclusion Bodies from the Liver in α₁-Antitrypsin Deficiency

1975 • 115 citations

Deformylation and protein biosynthesis

1969 • 96 citations

Cell-free synthesis of enterotoxin of E. coli from a cloned gene

1980 • 82 citations

Procollagen messenger RNA levels and activity and collagen synthesis during the fetal development of sheep lung, tendon, and skin.

1981 • 76 citations

α ₁ -Antitrypsin: The Presence of Excess Mannose in the Z Variant Isolated from Liver

1978 • 74 citations

Comparison of the chemical, physical, and survival properties of normal and Z-variant alpha-1-antitrypsins.

1976 • 48 citations

Cloning and Expression inEscherichia coliof Full-Length Complementary DNA Coding for Human α₁-Antitrypsin

1983 • 48 citations

Cloning of several cDNA segments coding for human liver proteins.

1983 • 40 citations

High-level synthesis in Escherichia coli of the SV40 small-t antigen under control of the bacteriophage lambda pl promoter

1982 • 36 citations

Silica gel: an improved support for the solid-phase phospphotriester synthesis of oligonucleotides

1982 • 30 citations

Induction of α1-antitrypsin mRNA and cloning of its cDNA

1981 • 27 citations

Amplification of the uvrA gene product of Escherichia coli to 7% of cellular protein by linkage to the pL promoter of pKC30.

1982 • 22 citations