Dissecting hypoxia‐dependent and hypoxia‐independent steps in the HIF‐1α activation cascade: implications for HIF‐1α gene therapy

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.

1995 • 5,971 citations

Targeting of HIF-α to the von Hippel-Lindau Ubiquitylation Complex by O ₂ -Regulated Prolyl Hydroxylation

2001 • 5,255 citations

The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis

1999 • 4,973 citations

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.

1992 • 4,885 citations

HIFα Targeted for VHL-Mediated Destruction by Proline Hydroxylation: Implications for O ₂ Sensing

2001 • 4,537 citations

Activation of Vascular Endothelial Growth Factor Gene Transcription by Hypoxia-Inducible Factor 1

1996 • 3,851 citations

Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours

1996 • 2,304 citations

Regulation of hypoxia-inducible factor 1α is mediated by an O ₂ -dependent degradation domain via the ubiquitin-proteasome pathway

1998 • 2,044 citations

Hypoxia-inducible Factor 1α (HIF-1α) Protein Is Rapidly Degraded by the Ubiquitin-Proteasome System under Normoxic Conditions

1997 • 1,610 citations

Ubiquitination of hypoxia-inducible factor requires direct binding to the β-domain of the von Hippel–Lindau protein

2000 • 1,527 citations

Modulation of hypoxia-inducible factor 1alpha expression by the epidermal growth factor/phosphatidylinositol 3-kinase/PTEN/AKT/FRAP pathway in human prostate cancer cells: implications for tumor angiogenesis and therapeutics.

2000 • 1,430 citations

HIF-1 and mechanisms of hypoxia sensing

2001 • 1,131 citations

Regulation of tumor angiogenesis by p53-induced degradation of hypoxia-inducible factor 1alpha.

2000 • 1,087 citations

Hypoxia Inducible Factor-α Binding and Ubiquitylation by the von Hippel-Lindau Tumor Suppressor Protein

2000 • 1,018 citations

Regulation of tumor angiogenesis by p53-induced degradation of hypoxia-inducible factor 1α

2000 • 893 citations

Mechanism of regulation of the hypoxia-inducible factor-1alpha by the von Hippel-Lindau tumor suppressor protein

2000 • 889 citations

Loss of PTEN facilitates HIF-1-mediated gene expression

2000 • 824 citations

Stabilization of wild-type p53 by hypoxia-inducible factor 1α

1998 • 818 citations

An essential role for p300/CBP in the cellular response to hypoxia

1996 • 780 citations

p42/p44 Mitogen-activated Protein Kinases Phosphorylate Hypoxia-inducible Factor 1α (HIF-1α) and Enhance the Transcriptional Activity of HIF-1

1999 • 777 citations

Regulation of the Hypoxia-inducible Transcription Factor 1α by the Ubiquitin-Proteasome Pathway

1999 • 744 citations

Angiogenesis Gene Therapy

1999 • 673 citations

Signal transduction in hypoxic cells: inducible nuclear translocation and recruitment of the CBP/p300 coactivator by the hypoxia-inducible factor-1alpha

1998 • 653 citations

Activation of HIF1α ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex

2000 • 635 citations

Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300

1999 • 583 citations

Induction of HIF-1alpha in response to hypoxia is instantaneous.

2001 • 507 citations

Mammalian Oxygen Sensing, Signalling and Gene Regulation

2000 • 453 citations

Functional Interference between Hypoxia and Dioxin Signal Transduction Pathways: Competition for Recruitment of the Arnt Transcription Factor

1996 • 449 citations

Induction of HIF–1α in response to hypoxia is instantaneous

2001 • 416 citations

Oxygen-regulated Transferrin Expression Is Mediated by Hypoxia-inducible Factor-1

1997 • 400 citations

Redox-Regulated Recruitment of the Transcriptional Coactivators CREB-Binding Protein and SRC-1 to Hypoxia-Inducible Factor 1α

2000 • 371 citations

ERK activation upon hypoxia: involvement in HIF‐1 activation

2000 • 365 citations

Reversal of experimental diabetic neuropathy by VEGF gene transfer

2001 • 302 citations

p53 Inhibits Hypoxia-inducible Factor-stimulated Transcription

1998 • 284 citations

Regulation of Transcription by Hypoxia Requires a Multiprotein Complex That Includes Hypoxia-Inducible Factor 1, an Adjacent Transcription Factor, and p300/CREB Binding Protein

1998 • 278 citations

Favorable effect of VEGF gene transfer on ischemic peripheral neuropathy

2000 • 235 citations

The transcription factors ATF-1 and CREB-1 bind constitutively to the hypoxia-inducible factor-1 (HIF-1)DNA recognition site

1995 • 228 citations

Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1α

1999 • 186 citations

Long-term expression of erythropoietin in the systemic circulation of mice after intramuscular injection of a plasmid DNA vector.

1996 • 181 citations

Hypoxia activates a platelet-derived growth factor receptor/phosphatidylinositol 3-kinase/Akt pathway that results in glycogen synthase kinase-3 inactivation.

2001 • 175 citations

Selection of human cervical epithelial cells that possess reduced apoptotic potential to low-oxygen conditions.

1997 • 147 citations

HIF‐1‐dependent transcriptional activity is required for oxygen‐mediated HIF‐1α degradation

2001 • 138 citations

Molecular Mechanism of Hypoxia-inducible Factor 1α-p300 Interaction

2001 • 125 citations

General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia‐inducible factor 1α

1999 • 122 citations

Mitogen-Activated Protein Kinase Kinase Inhibitor PD98059 Blocks thetrans-Activation but Not the Stabilization or DNA Binding Ability of Hypoxia-Inducible Factor-1α

2001 • 110 citations

Mersalyl Is a Novel Inducer of Vascular Endothelial Growth Factor Gene Expression and Hypoxia-Inducible Factor 1 Activity

1998 • 92 citations

Oxygen- and dioxin-regulated gene expression in mouse hepatoma cells

1997 • 89 citations

Up-regulation of hypoxia-inducible factor-1alpha is not sufficient for hypoxic/anoxic p53 induction.

1998 • 79 citations

Complex role of protein phosphorylation in gene activation by hypoxia

1997 • 71 citations

Regulation of vascular endothelial growth factor transcription by endothelial PAS domain protein 1 (EPAS1) and possible involvement of EPAS1 in the angiogenesis of renal cell carcinoma

2001 • 53 citations