The Gal3p-Gal80p-Gal4p Transcription Switch of Yeast: Gal3p Destabilizes the Gal80p-Gal4p Complex in Response to Galactose and ATP

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

A simplification of the protein assay method of Lowry et al. which is more generally applicable

1977 • 8,485 citations

The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases

1993 • 5,680 citations

Improved method for high efficiency transformation of intact yeast cells

1992 • 3,258 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Methods in Yeast Genetics: A Laboratory Course Manual

1990 • 1,922 citations

A new efficient gene disruption cassette for repeated use in budding yeast

1996 • 1,609 citations

GAL4-VP16 is an unusually potent transcriptional activator

1988 • 1,379 citations

Methods in Yeast Genetics — A Laboratory Course Manual

1991 • 1,257 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

Deletion analysis of GAL4 defines two transcriptional activating segments

1987 • 898 citations

Specific DNA binding of GAL4, a positive regulatory protein of yeast

1985 • 767 citations

A new class of yeast transcriptional activators

1987 • 706 citations

A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor

1985 • 686 citations

One-step transformation of yeast in stationary phase

1992 • 683 citations

DNA recognition by GAL4: structure of a protein-DNA complex

1992 • 664 citations

Separation of DNA Binding from the Transcription-Activating Function of a Eukaryotic Regulatory Protein

1986 • 626 citations

Transcriptional regulation in the yeast GAL gene family: a complex genetic network

1995 • 412 citations

[16] Analyzing protein-protein interactions using two-hybrid system

1995 • 364 citations

The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80

1987 • 349 citations

The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number

1983 • 300 citations

An amino-terminal fragment of GAL4 binds DNA as a dimer

1989 • 296 citations

[34] Ras-Raf interaction: Two-hybrid analysis

1995 • 287 citations

Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.

1984 • 284 citations

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.

1982 • 280 citations

Contact with a component of the polymerase II holoenzyme suffices for gene activation

1995 • 262 citations

Specific protein binding to far upstream activating sequences in polymerase II promoters.

1985 • 246 citations

Interaction of positive and negative regulatory proteins in the galactose regulon of yeast

1987 • 226 citations

An Activator Target in the RNA Polymerase II Holoenzyme

1998 • 195 citations

Quantitation of putative activator-target affinities predicts transcriptional activating potentials.

1996 • 195 citations

mRNA levels for the fermentative alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a nonfermentable carbon source.

1983 • 184 citations

The yeast galactose genetic switch is mediated by the formation of a Gal4p–Gal80p–Gal3p complex

1998 • 181 citations

GAL4 Interacts with TATA-Binding Protein and Coactivators

1995 • 164 citations

Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.

1987 • 161 citations

Analysis of theKluyveromyces lactispositive regulatory geneLAC9reveals functional homology to, but sequence divergence from, theSaccharomyces cerevisiae GALAgene

1986 • 158 citations

Activation of Gal4p by Galactose-Dependent Interaction of Galactokinase and Gal80p

1996 • 143 citations

Nondissociation of GAL4 and GAL80 in Vivo After Galactose Induction

1992 • 139 citations

Genetic evidence that an activation domain of GAL4 does not require acidity and may form a β sheet

1993 • 138 citations

Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes.

1984 • 129 citations

Functional analysis reports. Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction

1995 • 126 citations

Galactokinase encoded by GAL1 is a bifunctional protein required for induction of the GAL genes in Kluyveromyces lactis and is able to suppress the gal3 phenotype in Saccharomyces cerevisiae.

1991 • 121 citations

Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: evidence for a new model of induction for the yeast GAL/MEL regulon.

1992 • 114 citations

Coupling of cytosolic protein synthesis and mitochondrial protein import in yeast. Evidence for cotranslational import in vivo.

1993 • 109 citations

Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae

1977 • 109 citations

Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae

1997 • 104 citations

Analysis of the GAL3 signal transduction pathway activating GAL4 protein-dependent transcription in Saccharomyces cerevisiae.

1990 • 100 citations

Methods in Yeast Genetics — A Laboratory Course Manual.

1992 • 100 citations

Regulation of basal and induced levels of the MEL1 transcript in Saccharomyces cerevisiae.

1984 • 92 citations

Uninducible Mutants in the gal i Locus of Saccharomyces cerevisiae

1972 • 83 citations

Analysis of the Galactose Signal Transduction Pathway in Saccharomyces cerevisiae: Interaction between Gal3p and Gal80p

1996 • 80 citations

Galactose regulation in Saccharomyces cerevisiae

1979 • 79 citations

Novel Gal3 Proteins Showing Altered Gal80p Binding Cause Constitutive Transcription of Gal4p-Activated Genes in Saccharomyces cerevisiae

1997 • 77 citations

GENETIC AND MOLECULAR ANALYSIS OF THE GAL3 GENE IN THE EXPRESSION OF THE GALACTOSE/MELIBIOSE REGULON OF SACCHAROMYCES CEREVISIAE

1986 • 73 citations

Nucleotide sequence of the yeast regulatory geneGAL80

1984 • 66 citations

Mutational analysis of the GAL4-encoded transcriptional activator protein of Saccharomyces cerevisiae.

1988 • 66 citations

GAL4 is regulated by a glucose-responsive functional domain.

1993 • 61 citations

Efficient expression of the Saccharomyces cerevisiae glycolytic gene ADH1 is dependent upon a cis -acting regulatory element (UASRPG) found initially in genes encoding ribosomal proteins

1990 • 61 citations

A gene controlling inducibility of the galactose pathway enzymes in Saccharomyces

1963 • 60 citations

A transcriptional activating region with two contrasting modes of protein interaction

1998 • 57 citations

Molecular cloning of the GAL80 gene from Saccharomyces cerevisiae and characterization of a gal80 deletion

1984 • 57 citations

GAL4 mutations that separate the transcriptional activation and GAL80-interactive functions of the yeast GAL4 protein.

1990 • 54 citations

Gal80 Proteins of Kluyveromyces lactis and Saccharomyces cerevisiae Are Highly Conserved but Contribute Differently to Glucose Repression of the Galactose Regulon

1993 • 53 citations

5 Regulation of Carbon and Phosphate Utilization

1992 • 53 citations

Curing of Saccharomyces cerevisiae 2-?m DNA by transformation

1981 • 53 citations

GAL4 of Saccharomyces cerevisiae activates the lactose-galactose regulon of Kluyveromyces lactis and creates a new phenotype: glucose repression of the regulon.

1987 • 52 citations

Phosphorylation of Gal4p at a Single C-Terminal Residue Is Necessary for Galactose-Inducible Transcription

1996 • 52 citations

Simian virus 40 large T antigen contains two independent activities that cooperate with a ras oncogene to transform rat embryo fibroblasts

1995 • 49 citations

Transcription of the ADH2 gene in Saccharomyces cerevisiae is limited by positive factors that bind competitively to its intact promoter region on multicopy plasmids.

1987 • 46 citations

The organization and transcription of the galactose gene cluster ofKluyveromyceslactis

1988 • 43 citations

Interaction between transcriptional activator protein LAC9 and negative regulatory protein GAL80.

1989 • 35 citations

Signaling activation and repression of RNA polymerase II transcription in yeast

1997 • 35 citations

The DNA Binding and Activation Domains of Gal4p Are Sufficient for Conveying Its Regulatory Signals

1997 • 34 citations

The transcription factor LAC9 from Kluyveromyces lactis-like GAL4 from Saccharomyces cerevisiae forms a Zn(II)2Cys6 binuclear cluster.

1990 • 29 citations

[24] Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast

1990 • 29 citations

Autogenous regulation on the Saccharomyces cerevisiae regulatory gene GAL80

1987 • 29 citations

Expression of the transcriptional activator LAC9 (KlGAL4) in Kluyveromyces lactis is controlled by autoregulation.

1993 • 28 citations

Protein import into mitochondria in a homologous yeast in vitro system

1989 • 27 citations

GAL3 gene product is required for maintenance of the induced state of the GAL cluster genes in Saccharomyces cerevisiae

1986 • 25 citations

Autoregulation of GAl4 transcription is essential for rapid grwoth ofKluyveromyces lactison lactose and galactose

1993 • 25 citations

Regulated Phosphorylation of the Gal4p Inhibitor Gal80p of <I>Kluyveromyces lactis</I> Revealed by Mutational Analysis

1999 • 17 citations

Structural dissection of the DNA-binding domain of the yeast transcriptional activator GAL4 reveals an α-helical region responsible for dimerization

1998 • 2 citations