The Ubiquitin-Conjugating Enzyme Rad6 (Ubc2) Is Required for Silencing in Saccharomyces cerevisiae

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription

1990 • 1,343 citations

Shape representation in the inferior temporal cortex of monkeys

1995 • 979 citations

Handbook of genetics

1975 • 791 citations

Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: A molecular model for the formation of heterochromatin in yeast

1995 • 778 citations

TLC1 : Template RNA Component of Saccharomyces cerevisiae Telomerase

1994 • 727 citations

Four Genes Responsible for a Position Effect on Expression From HML and HMR in Saccharomyces cerevisiae

1987 • 709 citations

Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae

1991 • 700 citations

A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA

1989 • 550 citations

Spreading of transcriptional represser SIR3 from telomeric heterochromatin

1996 • 523 citations

Mutation in the silencing gene S/R4 can delay aging in S. cerevisiae

1995 • 496 citations

Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.

1993 • 463 citations

Inactivation of the HR6B Ubiquitin-Conjugating DNA Repair Enzyme in Mice Causes Male Sterility Associated with Chromatin Modification

1996 • 402 citations

SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres

1993 • 392 citations

The recognition component of the N-end rule pathway.

1990 • 388 citations

Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I.

1997 • 382 citations

Transcriptional silencing of Ty1 elements in the RDN1 locus of yeast.

1997 • 376 citations

Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers

1990 • 376 citations

Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites.

1994 • 318 citations

Loss of Transcriptional Silencing Causes Sterility in Old Mother Cells of S. cerevisiae

1996 • 308 citations

DNA REPAIR GENES AND PROTEINS OF SACCHAROMYCES CEREVISIAE

1993 • 301 citations

[5] Assay of yeast mating reaction

1991 • 286 citations

Saccharomyces telomeres assume a non-nucleosomal chromatin structure.

1992 • 275 citations

Regulated degradation of the transcription factor Gcn4.

1994 • 271 citations

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.

1991 • 268 citations

Overcoming telomeric silencing: a trans-activator competes to establish gene expression in a cell cycle-dependent way.

1994 • 267 citations

Silencers and Domains of Generalized Repression

1994 • 233 citations

A Deubiquitinating Enzyme Interacts with SIR4 and Regulates Silencing in S. cerevisiae

1996 • 228 citations

Telomere-proximal DNA in Saccharomyces cerevisiae is refractory to methyltransferase activity in vivo.

1992 • 227 citations

Histone H3 amino terminus is required for telomeric and silent mating locus repression in yeast

1994 • 227 citations

Silencing and Heritable Domains of Gene Expression

1995 • 196 citations

The RAD6 protein of Saccharomyces cerevisiae polyubiquitinates histones, and its acidic domain mediates this activity.

1988 • 196 citations

Active genes in budding yeast display enhanced in vivo accessibility to foreign DNA methylases: a novel in vivo probe for chromatin structure of yeast.

1992 • 188 citations

Molecular mechanisms of cell-type determination in budding yeast

1995 • 184 citations

Chapter 4 Preparation of RNA and Ribosomes from Yeast

1975 • 171 citations

11 Mating-type Determination and Mating-type Interconversion in Saccharomyces cerevisiae

1992 • 162 citations

Mutation of cysteine-88 in the Saccharomyces cerevisiae RAD6 protein abolishes its ubiquitin-conjugating activity and its various biological functions.

1990 • 155 citations

RAD6 gene of Saccharomyces cerevisiae encodes a protein containing a tract of 13 consecutive aspartates.

1985 • 151 citations

RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo.

1997 • 147 citations

Mutations in eukaryotic 18S ribosomal RNA affect translational fidelity and resistance to aminoglycoside antibiotics.

1994 • 135 citations

Yeast RAD6 encoded ubiquitin conjugating enzyme mediates protein degradation dependent on the N-end-recognizing E3 enzyme.

1991 • 115 citations

Domain structure and functional analysis of the carboxyl-terminal polyacidic sequence of the RAD6 protein of Saccharomyces cerevisiae.

1988 • 106 citations

Effects of altered 5'-flanking sequences on the in vivo expression of a Saccharomyces cerevisiae tRNATyr gene.

1984 • 104 citations

Replication and Segregation of Plasmids Containing cis-Acting Regulatory Sites of Silent Mating-Type Genes in Saccharomyces cerevisiae Are Controlled by the SIR Genes

1987 • 100 citations

The extremely conserved amino terminus of RAD6 ubiquitin-conjugating enzyme is essential for amino-end rule-dependent protein degradation.

1993 • 82 citations

Ubiquitin-mediated degradation of histone H3 does not require the substrate-binding ubiquitin protein ligase, E3, or attachment of polyubiquitin chains.

1990 • 75 citations

Mutations in RAD6, a yeast gene encoding a ubiquitin-conjugating enzyme, stimulate retrotransposition.

1990 • 69 citations

Stable ester conjugate between the Saccharomyces cerevisiae RAD6 protein and ubiquitin has no biological activity

1991 • 58 citations

The N-end Rule

1995 • 56 citations

Interaction of the yeast RAD7 and SIR3 proteins: implications for DNA repair and chromatin structure.

1994 • 51 citations

The Dose of a Putative Ubiquitin-Specific Protease Affects Position-Effect Variegation in Drosophila melanogaster

1996 • 51 citations

Disturbance of Normal Cell Cycle Progression Enhances the Establishment of Transcriptional Silencing in Saccharomyces cerevisiae

1995 • 47 citations

A ubiquitin-conjugating enzyme, RAD6, affects the distribution of Ty1 retrotransposon integration positions.

1993 • 47 citations

Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure.

1995 • 39 citations

Cloning and integrative deletion of the RAD6 gene of Saccharomyces cerevisiae

1984 • 31 citations