Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: A molecular model for the formation of heterochromatin in yeast

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Improved method for high efficiency transformation of intact yeast cells

1992 • 3,258 citations

Multifunctional yeast high-copy-number shuttle vectors

1992 • 1,656 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription

1990 • 1,343 citations

Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae

1991 • 700 citations

Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements

1987 • 637 citations

Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei

1992 • 552 citations

Evidence that a complex of SIR proteins interacts with the silencer and telomere-binding protein RAP1.

1994 • 547 citations

Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.

1988 • 511 citations

Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.

1993 • 463 citations

Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.

1992 • 461 citations

Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast

1988 • 441 citations

SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres

1993 • 392 citations

Genetic evidence for an interaction between SIR3 and histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae.

1990 • 350 citations

Genetic Analysis of Histone H4: Essential Role of Lysines Subject to Reversible Acetylation

1990 • 346 citations

Yeast histone H4 N-terminal sequence is required for promoter activation in vivo

1991 • 330 citations

The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB.

1993 • 317 citations

Silencers, silencing, and heritable transcriptional states

1992 • 296 citations

RAP1 and telomere structure regulate telomere position effects in Saccharomyces cerevisiae.

1993 • 283 citations

Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast

1992 • 280 citations

Saccharomyces telomeres assume a non-nucleosomal chromatin structure.

1992 • 275 citations

Role of DNA replication in the repression of silent mating type loci in yeast

1984 • 254 citations

Histone H3 amino terminus is required for telomeric and silent mating locus repression in yeast

1994 • 227 citations

Effects of histone H4 depletion on the cell cycle and transcription of Saccharomyces cerevisiae.

1988 • 225 citations

Targeting of SIR1 protein establishes transcriptional silencing at HM loci and telomeres in yeast

1993 • 207 citations

Histone H3 N-terminal mutations allow hyperactivation of the yeast GAL1 gene in vivo.

1992 • 186 citations

RAP1 protein activates and silences transcription of mating-type genes in yeast.

1991 • 170 citations

Notes: Point Mutations in the Yeast Histone H4 Gene Prevent Silencing of the Silent Mating Type Locus HML

1990 • 168 citations

Position effect variegation in Drosophila: Towards a genetics of chromatin assembly

1989 • 134 citations

A highly basic histone H4 domain bound to the sharply bent region of nucleosomal DNA

1988 • 123 citations

Identification of a non-basic domain in the histone H4 N-terminus required for repression of the yeast silent mating loci.

1992 • 120 citations

Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.

1990 • 108 citations

Functional domains of SIR4, a gene required for position effect regulation in Saccharomyces cerevisiae.

1987 • 105 citations

Yeast histone H2A and H2B amino termini have interchangeable functions

1986 • 88 citations

HISTONE GENE MULTIPLICITY AND POSITION EFFECT VARIEGATION IN DROSOPHILA MELANOGASTER

1983 • 80 citations

Silencers, silencing, and heritable transcriptional states.

1992 • 78 citations

A strategy for efficientin vitrotranslation of cDNAs using the rabbit β-globin leader sequence

1991 • 61 citations

A single histone acetyltransferase from Tetrahymena macronuclei catalyzes deposition-related acetylation of free histones and transcription-related acetylation of nucleosomal histones.

1987 • 58 citations

Histones and the Regulation of Heterochromatin in Yeast

1993 • 53 citations

Mechanisms for the construction and developmental control of heterochromatin formation and imprinted chromosome domains

1990 • 46 citations

Silencing speaks up

1994 • 26 citations