Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators

Experiments in molecular genetics

1972 • 28,260 citations

Methods in Enzymology , Vol

1966 • 26,261 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

1974 • 3,603 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTION

1979 • 2,730 citations

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1980 • 2,499 citations

Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322

1979 • 1,807 citations

Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli

1981 • 1,732 citations

The tac promoter: a functional hybrid derived from the trp and lac promoters.

1983 • 1,001 citations

Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli

1983 • 810 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA

1974 • 541 citations

A bacterial clone synthesizing proinsulin.

1978 • 515 citations

Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli

1981 • 476 citations

Plasmid vectors for the selection of promoters

1984 • 428 citations

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

1978 • 426 citations

Mechanism of action of the lexA gene product.

1981 • 424 citations

A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.

1981 • 417 citations

Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

1982 • 281 citations

Purified λ regulatory protein cII positively activates promoters for lysogenic development

1981 • 241 citations

Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro.

1982 • 210 citations

Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

1980 • 203 citations

Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities

1975 • 190 citations

Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.

1981 • 177 citations

Bacteria mature preproinsulin to proinsulin.

1980 • 143 citations

Cellular location affects protein stability in Escherichia coli.

1982 • 140 citations

A mechanism of protein localization: the signal hypothesis and bacteria.

1980 • 127 citations

Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.

1978 • 106 citations

Plasmid vectors containing the tryptophan operon promoter suitable for efficient regulated expression of foreign genes

1980 • 88 citations

Specificity of the bacteriophage lambda N gene product (pN): Nut sequences are necessary and sufficient for antitermination by pN

1979 • 86 citations

Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region

1980 • 82 citations

Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids

1979 • 77 citations

Sequences of the Escherichia coli dnaG primase gene and regulation of its expression.

1982 • 74 citations

Transcription termination in the Escherichia coli ribosomal RNA operon rrnC.

1979 • 72 citations

An ‘internal” signal sequence directs secretion and processing of proinsulin in bacteria

1981 • 68 citations

Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli.

1981 • 61 citations

The distal end of the ribosomal RNA operon rrnD of Eschenchia coli contains a tRNA₁^thrgene, two 5S rRNA genes and a transcription terminator

1980 • 61 citations

Nudeotide sequence at the end of the gene for the RNA polymerase β subunit (rpoC)

1981 • 60 citations

Effect of strong promoters on the cloning in Escherichia coli of DNA fragments from Streptococcus pneumoniae

1982 • 58 citations

Restriction map of the Escherichia coli malA region and identification of the malT product

1980 • 54 citations

Transposition of a chromosomal segment bounded by redundant rRNA genes into other rRNA genes in Escherichia coli

1982 • 51 citations

Sequence of the distal tRNA₁^Aspgene and the transcription termination signal in the Escherichia coli ribosomal RNA operon rrnF(or G)

1980 • 46 citations

Lambda phage promoter used to enhance expression of a plasmid-cloned gene

1978 • 37 citations

Cloning and characterization of the gene for Salmonella typhimurium serine hydroxymethyltransferase

1984 • 25 citations

Construction and characterization of a plasmid coding for a fragment of the Escherichia coli recA protein

1979 • 20 citations

The construction of new vehicles for the cloning of transcription termination signals

1981 • 13 citations