Nucleotide sequence of tbe hygromycin B phosphotransferase gene fromStreptomyces hygroscopicus

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Improved Estimation of Secondary Structure in Ribonucleic Acids

1973 • 2,302 citations

A gas-liquid solid phase peptide and protein sequenator.

1981 • 2,238 citations

[68] Transcription maps of polyoma virus-specific RNA: Analysis by two-dimensional nuclease S1 gel mapping

1980 • 1,430 citations

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1983 • 1,115 citations

Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae

1983 • 810 citations

Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli

1984 • 745 citations

Aminoglycoside Antibiotic-Inactivating Enzymes in Actinomycetes Similar to Those Present in Clinical Isolates of Antibiotic-Resistant Bacteria

1973 • 497 citations

Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene.

1981 • 485 citations

pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors

1982 • 415 citations

[43] Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography

1983 • 358 citations

Extraction of nucleic acids from agarose gels

1980 • 346 citations

Gene expression in Streptomyces: Construction and application of promoter-probe plasmid vectors in Streptomyces lividans

1982 • 316 citations

Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells.

1984 • 230 citations

Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction

1982 • 229 citations

The Streptomyces plasmid SCP2∗: its functional analysis and development into useful cloning vectors

1985 • 208 citations

Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces

1985 • 180 citations

Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H.

1984 • 177 citations

The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product

1985 • 171 citations

RNA polymerase heterogeneity in Streptomyces coelicolor

1985 • 167 citations

Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.

1983 • 157 citations

Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli

1983 • 95 citations

Streptomyces lividans RNA polymerase recognizes and uses Escherichia coli transcriptional signals

1984 • 77 citations

Molecular cloning and expression in Streptomyces lividans of a hygromycin B phosphotransferase gene from Streptomyces hygroscopicus

1983 • 47 citations

Biochemical Basis of Resistance to Hygromycin B in Streptomyces hygroscopicus - the Producing Organism

1985 • 42 citations

Elimination of the secondary structure effect in gel sequencing of nucleic acids

1980 • 35 citations