Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli

Methods in Enzymology , Vol

1966 • 26,261 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Selective extraction of polyoma DNA from infected mouse cell cultures

1967 • 5,357 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

Rapid procedure for detection and isolation of large and small plasmids

1981 • 2,840 citations

Transduction of linked genetic characters of the host by bacteriophage P1

1955 • 2,703 citations

Rapid and efficient cosmid cloning

1981 • 1,897 citations

[17] A rapid alkaline extraction method for the isolation of plasmid DNA

1983 • 983 citations

Lambdoid phages that simplify the recovery of in vitro recombinants

1977 • 779 citations

Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event

1970 • 770 citations

General Method for the Isolation of Plasmid Deoxyribonucleic Acid

1973 • 725 citations

A simple method for the preparation of large quantities of pure plasmid DNA

1975 • 586 citations

Identification and Characterization of Large Plasmids in Rhizobium meliloti using Agarose Gel Electrophoresis

1979 • 574 citations

The twisted circular form of polyoma viral DNA.

1965 • 551 citations

Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

1978 • 514 citations

Plasmids, Recombination and Chromosome Mapping in Streptomyces lividans 66

1983 • 416 citations

pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors

1982 • 415 citations

Isolation of covalently closed circular DNA of high molecular weight from bacteria

1976 • 409 citations

Effect of Growth Conditions on the Formation of the Relaxation Complex of Supercoiled ColE1 Deoxyribonucleic Acid and Protein in Escherichia coli

1972 • 351 citations

Gene Cloning in Streptomyces

1982 • 335 citations

Studies on the selectivity of DNA precipitation by spermine

1981 • 267 citations

A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder

1982 • 259 citations

A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis

1980 • 234 citations

Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3(2)

1977 • 231 citations

Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction

1982 • 229 citations

Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography

1978 • 225 citations

A new method for the purification and identification of covalently closed circular DNA molecules

1978 • 210 citations

Rapid Methods for the Study of both Stable and Unstable Plasmids in Pseudomonas

1981 • 160 citations

Simple procedure for distinguishing CCC, OC, and L forms of plasmid DNA by agarose gel electrophoresis

1981 • 143 citations

Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans

1981 • 142 citations

Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis

1980 • 139 citations

Genetics of antibiotic production

1977 • 116 citations

A LINEAR PLASMID-LIKE DNA IN STREPTOMYCES SP. PRODUCING LANKACIDIN GROUP ANTIBIOTICS

1979 • 99 citations

Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2)

1975 • 98 citations

Analysis of linear plasmids isolated from Streptomyces: Association of protein with the ends of the plasmid DNA

1982 • 89 citations

Preparation of double-stranded DNA (replicative form) of bacteriophage ΦX174: A simplified method

1966 • 78 citations

Genetic Control of Fertility in Streptomyces coelicolor A3(2): Plasmid Involvement in the Interconversion of UF and IF Strains

1971 • 73 citations

Genetics of antibiotic production.

1977 • 72 citations

The effect of spermidine on endonuclease inhibition by agarose contaminants

1981 • 49 citations

Isolation and characterization of plasmid DNAs in Actinomycetes.

1980 • 46 citations

Determination of Methylenomycin A Synthesis by the pSV1 Plasmid from Streptomyces violaceus-ruber SANK 95570

1982 • 44 citations

Cloning Streptomyces genes for antibiotic production

1983 • 43 citations

Isolation of Inc P-2 plasmid DNA from Pseudomonas aeruginosa

1978 • 42 citations

Characterization of a plasmid from Streptomyces coelicolor A3(2)

1977 • 40 citations

Studies on new aminoglycoside antibiotics, istamycins, from an actinomycete isolated from a marine environment. I. The use of plasmid profiles in screening antibiotic-producing streptomycetes.

1980 • 36 citations

Development of cloning vehicles from the Streptomyces plasmid pFJ103

1982 • 36 citations

Isolation and characterization of Streptomyces fradiae plasmids which are prophage of the actinophage øSF1

1982 • 33 citations

Extraction and characterization of plasmids from macrolide antibiotic-producing Streptomycetes.

1981 • 31 citations

Structure of the replicative form of bacteriophage φX174

1969 • 26 citations

Basic techniques for DNA cloning and conditions required for streptomycetes as a host.

1983 • 23 citations

Isolation and characterization of plasmids from parent and variant strains of Streptomyces ribosidificus.

1980 • 19 citations

Preparative method for the isolation of super-coiled DNA from a chloramphenicol-producing Streptomycete.

1977 • 18 citations

Isolation and physical characterization of streptomycete plasmids

1981 • 18 citations

Purification of episomal DNA with cellulose nitrate membrane filters

1966 • 17 citations

Physical characterization of plasmids from Streptomyces kasugaensis MB273

1981 • 16 citations

Restriction enzyme map for streptomycete plasmid pUC3

1979 • 16 citations

A cryptic plasmid in the chloramphenicol-producing actinomycete,Streptomyces phaeochromogenes

1983 • 15 citations

Plasmid DNA in the erythromycin producing microorganism, Streptomyces erythreus NRRL 2338.

1982 • 14 citations

ISOLATION AND CHARACTERIZATION OF COVALENTLY CLOSED CIRCULAR DNA ASSOCIATED WITH CHROMOSOMAL AND MEMBRANE FRACTION FROM STREPTOMYCES AMBOFACIENS

1982 • 12 citations

A survey of plasmids among natural isolates of Streptomyces.

1983 • 12 citations

Isolation and characterization of small plasmids from Streptomyces.

1982 • 10 citations

Partial characterization of a small, multi-copy plasmid from Streptomyces espinosus and the derivation of a high copy-number deletion mutant

1982 • 9 citations

Fluorescence spot tests for DNA endonuclease, ligase, and topoisomerase activities

1980 • 9 citations

A simple method for the qualitative demonstration of large and small plasmid deoxyribonucleic acids in Gram-positive and Gram-negative bacteria

1982 • 9 citations

Studies on new aminoglycoside antibiotics, istamycins, from an actinomycete isolated from a marine envioment. II. Possible involvement of plasmid in istamycin production.

1980 • 9 citations

Aspects of the mechanism of acid-phenol extraction of nucleic acids

1983 • 8 citations