Oligodeoxynucleotides as Antisense Inhibitors of Gene Expression

CRC Handbook of Chemistry and Physics

1992 • 9,920 citations

Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide.

1978 • 1,668 citations

Antisense oligonucleotides: a new therapeutic principle

1990 • 1,616 citations

Principles of Nucleic Acid Structure

1985 • 1,497 citations

Anti-cancer Drug Design

2018 • 1,309 citations

Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes

1981 • 1,265 citations

Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles

1983 • 1,256 citations

Simple RNA enzymes with new and highly specific endoribonuclease activities

1988 • 1,118 citations

Oncogenes, antioncogenes, and the molecular bases of multistep carcinogenesis.

1989 • 961 citations

FORMATION OF A THREE-STRANDED POLYNUCLEOTIDE MOLECULE

1957 • 900 citations

Inhibition of Rous sarcoma viral RNA translation by a specific oligodeoxyribonucleotide.

1978 • 841 citations

How do eucaryotic ribosomes select initiation regions in messenger RNA?

1978 • 830 citations

Characterization of oligonucleotide transport into living cells.

1989 • 739 citations

Site-Specific Oligonucleotide Binding Represses Transcription of the Human c- myc Gene in Vitro

1988 • 707 citations

A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1

1987 • 696 citations

Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.

1986 • 684 citations

HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA

1986 • 673 citations

Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes

1988 • 617 citations

Physicochemical properties of phospborothioate oligodeoxynucleotides

1988 • 567 citations

Pharmacokinetics, biodistribution, and stability of oligodeoxynucleotide phosphorothioates in mice.

1991 • 551 citations

Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

1977 • 543 citations

Phosphorothioate analogs of oligodeoxynucleotides: inhibitors of replication and cytopathic effects of human immunodeficiency virus.

1987 • 530 citations

Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?

1989 • 510 citations

An oligomer complementary to c-myc mRNA inhibits proliferation of HL-60 promyelocytic cells and induces differentiation.

1988 • 476 citations

The generality of self-splicing RNA: Relationship to nuclear mRNA splicing

1986 • 464 citations

Role of RNase H in hybrid-arrested translation by antisense oligonucleotides.

1988 • 445 citations

A c- myb Antisense Oligodeoxynucleotide Inhibits Normal Human Hematopoiesis in Vitro

1988 • 439 citations

The primary structure of rabbit β-globin mRNA as determined from cloned DNA

1977 • 428 citations

Inhibition of Cellular Proliferation by Antisense Oligodeoxynucleotides to PCNA Cyclin

1988 • 425 citations

Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

1985 • 410 citations

Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.

1986 • 403 citations

Conjugates of oligonucleotides and modified oligonucleotides: a review of their synthesis and properties

1990 • 400 citations

Extension of the Life-Span of Human Endothelial Cells by an Interleukin-1αAntisense Oligomer

1990 • 399 citations

Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.

1988 • 374 citations

Regulation of Gene Expression with Double-Stranded Phosphorothioate Oligonucleotides

1990 • 373 citations

Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides

1987 • 356 citations

Selective Inhibition of Leukemia Cell Proliferation by BCR-ABL Antisense Oligodeoxynucleotides

1991 • 353 citations

Synthesis of DNA via deoxynudeoside H-phosphonate Intermediates

1986 • 343 citations

Specific antiviral activity of a poly(L-lysine)-conjugated oligodeoxyribonucleotide sequence complementary to vesicular stomatitis virus N protein mRNA initiation site.

1987 • 329 citations

Antisense-mediated inhibition of BCL2 protooncogene expression and leukemic cell growth and survival: comparisons of phosphodiester and phosphorothioate oligodeoxynucleotides.

1990 • 299 citations

Cholesteryl-conjugated oligonucleotides: synthesis, properties, and activity as inhibitors of replication of human immunodeficiency virus in cell culture.

1989 • 296 citations

Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.

1986 • 295 citations

Oligodeoxynucleoside phosphoramidates and phosphorothioates as inhibitors of human immunodeficiency virus.

1988 • 289 citations

Automated solid-phase synthesis, separation, and stereochemistry of phosphorothioate analogs of oligodeoxyribonucleotides

1984 • 272 citations

Highly efficient chemical synthesis of 2′-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases

1989 • 268 citations

Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells.

1989 • 264 citations

Epithelial-mesenchymal transformation of embryonic cardiac endothelial cells is inhibited by a modified antisense oligodeoxynucleotide to transforming growth factor beta 3.

1991 • 263 citations

Sequence‐dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H

1987 • 261 citations

α-Oligodeoxynucleotide stability in serum, subcellular extracts and culture media

1988 • 257 citations

Important Advances in Oncology 1988

1988 • 253 citations

The use of single-stranded DNA and RNase H to promote quantitative ‘hybrid arrest of translation’ of mRNA/DNA hybrids in reticulocyte lysate cell-free translations

1986 • 245 citations

DNA binding to human leukocytes. Evidence for a receptor-mediated association, internalization, and degradation of DNA.

1985 • 240 citations

Comparative inhibition of rabbit globin mRNA translation by modified antisense oligodeoxynucleotides

1989 • 238 citations

pH-sensitive immunoliposomes mediate target-cell-specific delivery and controlled expression of a foreign gene in mouse.

1987 • 235 citations

Inhibition of human immunodeficiency virus replication by antisense oligodeoxynucleotides.

1988 • 227 citations

Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.

1987 • 225 citations

Oligodeoxynucleotide stability in subcellular extracts and culture media

1986 • 224 citations

Evaluation of the “scanning model” for initiation of protein synthesis in eucaryotes

1980 • 223 citations

Normal and leukemic hematopoietic cells manifest differential sensitivity to inhibitory effects of c-myb antisense oligodeoxynucleotides: an in vitro study relevant to bone marrow purging.

1991 • 214 citations

Influence of mRNA secondary structure on binding and migration of 40S ribosomal subunits

1980 • 194 citations

Left-handed DNA: from Synthetic Polymers to Chromosomes

1983 • 181 citations

Site-specific excision from RNA by RNase H and mixed-phosphate-backbone oligodeoxynucleotides.

1990 • 177 citations

Mouse Mos protooncogene product is present and functions during oogenesis.

1989 • 173 citations

Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkages

1987 • 173 citations

A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate.

1979 • 172 citations

Enzymatic amplification of translation inhibition of rabbit β-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents

1987 • 164 citations

The 35-Nucleotide Spliced Leader Sequence Is Common to All Trypanosome Messenger RNA's

1986 • 164 citations

Rapid nuclear accumulation of injected oligodeoxyribonucleotides.

1990 • 157 citations

Stereochemistry of RNA Cleavage by the Tetrahymena Ribozyme and Evidence that the Chemical Step Is Not Rate-Limiting

1989 • 155 citations

Influence of 5' proximal secondary structure on the translational efficiency of eukaryotic mRNAs and on their interaction with initiation factors.

1986 • 149 citations

Synthesis of alkylating oligonucleotide derivatives containing cholesterol or phenazinium residues at their 3′‐terminus and their interaction with DNA within mammalian cells

1989 • 147 citations

Crystallographic refinement and structure of DNase I at 2 Å resolution

1986 • 145 citations

Rate of degradation of [α]- and [β]-oligodeoxynucleotides inXenopusoocytes. Implications for anti-messenger strategies

1987 • 143 citations

Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length

1986 • 142 citations

Geometry of the First Step in the Action of Ribonuclease-A

1972 • 142 citations

Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.

1988 • 136 citations

Mechanism of mRNA Recognition by Eukaryotic Ribosomes During Initiation of Protein Synthesis

1981 • 134 citations

Secondary structure of mouse and rabbit α- and β-globin mRNAs: Differential accessibility of α and β initiator AUG codons towards nucleases

1980 • 133 citations

Deoxynucleoside H-Phosphonate diester intermediates in the synthesis of internucleotide phosphate analogues

1986 • 132 citations

Inhibition of acquired immunodeficiency syndrome virus by oligodeoxynucleoside methylphosphonates.

1988 • 132 citations

Selective inhibition of the cytopathic effect of type A influenza viruses by oligodeoxynucleotides covalently linked to an intercalating agent

1987 • 131 citations

Α-DNA II. Synthesis of unnatural Α-anomeric oligodeoxyribonucleotides containing the four usual bases and study of their substrate activities for nucleases

1987 • 130 citations

Phosphorothioate analogs of nucleotides

1979 • 127 citations

Site-directed cleavage of RNA

1987 • 124 citations

Oligodeoxynucleotides: Antisense inhibitors of gene expression

1990 • 124 citations

Phosphorothioate and normal oligodeoxyribonucleotides with 5′-linked acridine: characterization and preliminary kinetics of cellular uptake

1988 • 123 citations

Separation of Calf Thymus Deoxyribonucleic Acid into Fractions of Different Composition

1953 • 122 citations

α-DNA VI: comparative study of α- and β-anomeric oligodeoxyribonucleotides in hybridization to mRNA and in cell free translation inhibition

1987 • 121 citations

Specific inhibition of c-myc protein biosynthesis using an antisense synthetic deoxy-oligonucleotide in human T lymphocytes.

1988 • 119 citations

Formation of the triple-stranded polynucleotide helix, poly(A.A.U).

1987 • 119 citations

Control of ribonucleic acid function by oligonucleoside methylphosphonates

1985 • 115 citations

Chiral phosphorothioate analogues of B-DNA

1986 • 114 citations

Translationally associated helix-destabilizing activity in rabbit reticulocyte lysate.

1984 • 111 citations

Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylpbosphonates in a cell-free system

1988 • 110 citations

Differentiation of HL-60 leukemia by type I regulatory subunit antisense oligodeoxynucleotide of cAMP-dependent protein kinase.

1991 • 108 citations

Selective inhibition of Escherichia coli protein synthesis and growth by nonionic oligonucleotides complementary to the 3′ end of 16S rRNA

1981 • 108 citations

Delivery of c-myc Antisense Phosphorothioate Oligodeoxynucleotides to Hematopoietic Cells in Culture by Liposome Fusion: Specific Reduction in c-myc Protein Expression Correlates with Inhibition of Cell Growth and DNA Synthesis

1988 • 102 citations

Stereochemical Course of Catalysis by the Tetrahymena Ribozyme

1989 • 99 citations

α-DNA I. Synthesis, characterization by high field¹H-NMR, and base-pairing properties of the unnatural hexadeoxyribonudeotide α-[d(CpCpTpTpCpC)] with its complement β-[d(GpGpApApGpG)]

1986 • 98 citations

Inhibition of human immunodeficiency virus (HIV-1) replication by synthetic oligo-RNA derivatives

1989 • 98 citations

A catalytic 13-mer ribozyme

1989 • 92 citations

The common 5′ terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides

1986 • 91 citations

Antiviral activity of conjugates between poly(l-lysine) and synthetic oligodeoxyribonucleotides

1988 • 91 citations

Investigation of Enzyme Mechanisms with Nucleoside Phosphorothioates

1975 • 89 citations

Inhibition of herpes simplex virus type 2 growth by phosphorothioate oligodeoxynucleotides

1990 • 87 citations

An acridine-linked oligodeoxynucleotide targeted to the common 5' end of trypanosome mRNAs kills cultured parasites

1987 • 87 citations

Inhibition of human immunodeficiency virus by using an oligonucleoside methylphosphonate targeted to the tat-3 gene

1988 • 85 citations

A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deoxyadenosine triphosphate.

1982 • 82 citations

α-DNA-V. Parallel annealing, handedness and conformation of the duplex of the unnatural α-hexadeoxyribonucleotide α-[d(CpApTpGpCpG)] with its β-complement β-[d(GpTpApCpGpC)] deduced from high field¹H-NMR

1987 • 81 citations

Phosphorothioate-modified oligodeoxyribonucleotides. III. NMR and UV spectroscoptc studies of theR_p-R_p,S_p-S_p, andR_p-S_pduplexes, [d(GG_sAATTCC)]₂, derived from diastereomericO-ethyl phosphorothioates

1986 • 80 citations

Double helices with parallel strands are formed by nuclease-resistant oligo-[α]-deoxynucleotides and oligo-[α]-deoxynucleotides covalently linked to an intercalating agent with complementary oligo-[β]-deoxynucleotides

1987 • 79 citations

Deoxyoligonucleotide analogs based on formacetal linkages

1990 • 75 citations

Inhibition of Human Immunodeficiency Virus Type 1-mediated Cytopathic Effects by Poly(L-lysine)-conjugated Synthetic Antisense Oligodeoxyribonucleotides

1989 • 74 citations

Analysis of mRNA populations by cDNA·mRNA hybrid-mediated inhibition of cell-free protein synthesis

1978 • 72 citations

mRNA secondary structure as a determinant in cap recognition and initiation complex formation. ATP-Mg2+ independent cross-linking of cap binding proteins to m7I-capped inosine-substituted reovirus mRNA.

1983 • 70 citations

Destabilization of messenger RNA/complementary DNA duplexes by the elongating 80 S ribosome.

1986 • 70 citations

Synthesis of phosphorothioate analogues of oligodeoxyribonucleotides and their antiviral activity against human immunodeficiency virus (HIV)

1988 • 68 citations

α-DNA IX: parallel annealing of α-anomeric oligodeoxyribonucleotides to natural mRNA is required for interference in RNase H mediated hydrolysis and reverse transcription

1989 • 65 citations

The inhibition of neunte outgrowth in PC12 cells by tubulin antisense oligodeoxyribonucleotides

1988 • 63 citations

Oligo-[α]-deoxynucleotides covalently linked to an intercalating agent. Double helices with parallel strands are formed with complementary oligo-[β]-deoxynucleotides

1987 • 63 citations

Complementary oligodeoxynucleotide mediated inhibition of tobacco mosaic virus RNA translationin vitro

1988 • 61 citations

Synthetic repeating sequence DNAs containing plsosphorothioates: nuclease sensitivity and triplex formation

1989 • 61 citations

Antisense oligodeoxynucleotides provide insight into mechanism of translation initiation of two Sendai virus mRNAs.

1987 • 60 citations

Stereochemical course of polymerization catalyzed by avian myeloblastosis virus reverse transcriptase.

1982 • 57 citations

Stereochemical course of DNA hydrolysis by nuclease S1.

1983 • 57 citations

Oligodeoxynucleotides as Anti-Sense Inhibitors of Gene Expression: Therapeutic Implications

1989 • 57 citations

The antiviral activity of thiophosphate-substituted polyribonucleotides in vitro and in vivo

1970 • 56 citations

Target dependence of antisense oligodeoxynucleotide inhibition of c-Ha-ras p21 expression and focus formation in T24-transformed NIH3T3 cells.

1990 • 55 citations

Anti-messenger oligodeoxynucleotides: specific inhibition of rabbit β-globin synthesis in wheat germ extracts and Xenopus oocytes

1986 • 52 citations

Antisense inhibition of single copy N-myc expression results in decreased cell growth without reduction of c-myc protein in a neuroepithelioma cell line.

1990 • 52 citations

Uptake of polynucleotides by intact mammalian cells

1971 • 50 citations

Synthesis and characterization of nucleoside peptides: toward chemical ribonucleases. 1

1990 • 50 citations

Synthesis of 2'-O-Methyluridine, 2'-O-Methylcytidine and Their Relating Compounds.

1965 • 48 citations

Antiviral activity and possible mechanisms of action of oligonucleotides-poly(L-lysine) conjugates targeted to vesicular stomatitis virus mRNA and genomic RNA

1989 • 47 citations

α-DNA VIII: thermodynamic parameters of complexes formed between the oligo-alpha-deoxynucleotides: α-d(GGAAGG) and α-d(CCTTCC) and their complementary oligo-beta-deoxynucleotides: β-d(CCTTCC) and β-d(GGAAGG) are different

1989 • 46 citations

Oligodeoxynucleotide analogs with 5′‐linked anthraquinone

1989 • 44 citations

Configuration of β-globin messenger RNA in rabbit reticulocytes

1984 • 43 citations

Conformational perturbation due to an extra adenosine in a self‐complementary oligodeoxynucleotide duplex

1987 • 43 citations

The 3′ noncoding region of β-globin mRNA is not essential for in vitro translation

1979 • 43 citations

Nucleosides and nucleotides. Part 5. The stereochemistry of oligonucleotides consisting of 2′-deoxy-α-d-ribosides, a study withDreiding stereomodels

1973 • 42 citations

Evaluation of N-ras oncogene anti-sense, sense and nonsense sequence methylphosphonate oligonucleotide analogues.

1988 • 42 citations

Effect of a single 3′-methylene phosphonate linkage on the conformation of an A-DNA octamer double helix

1991 • 39 citations

Preparation of a deoxynucleoside thiophosphoramidite intermediate in the synthesis of nucleoside phosphorodithioates

1988 • 38 citations

Nucleosides and Nucleotides. Part 7. Four dithymidine monophosphates with different anomeric configurations, their synthesis and behaviour towards phosphodiesterases

1974 • 38 citations

Oligodeoxynucleotides containing unnatural L-2′-deoxyribose

1991 • 38 citations

Designing antisense oligonucleotides aspharmaceutical agents

1989 • 37 citations

Concerning Antisense Inhibition of the Multiple Drug Resistance Gene

1990 • 37 citations

Synthesis of dinucleoside phosphorodithioates via thioamidites

1988 • 36 citations

Site specific functionalization of oligonucleotides for attaching two different reporter groups

1990 • 35 citations

Nucleosides and Nucleotides. Part 2: Synthesis of both anomers of 1‐(5′‐O‐phosphoryl‐2′‐deoxy‐<scp>D</scp>‐ribofuranosyl)‐2(1H)‐pyridone [1]

1972 • 34 citations

Complementary Oligonucleotide Probe of Vesicular Stomatitus Virus Matrix Protein mRNA Translation

1986 • 34 citations

Preparation of 35S-labeled polyphosphorothioate oligodeoxyribonucleotides by use of hydrogen phosphonate chemistry

1990 • 33 citations

Antiviral activity in L1210 cells of liposome‐encapsulated (2′‐5′)oligo(adenylate) analogues

1985 • 33 citations

Deoxyribonucleoside Phosphorodithioates. Preparation of Dinucleoside Phosphorodithioates from Nucleoside Thiophosphoramidites.

1989 • 31 citations

Antisense oligonucleotide inhibition of encephalomyocarditis virus RNA translation

1989 • 30 citations

Comparative inhibition of ras p21 protein synthesis with phosphorus-modified antisense oligonucleotides.

1989 • 30 citations

Detection of lipopolysaccharides blotted to polyvinylidene difluoride membranes

1990 • 27 citations

Translation arrest of potato virus X RNA in Krebs‐2 cell‐free system: RNase H cleavage promoted by complementary oligodeoxynucleotides

1988 • 27 citations

Effect of RNA secondary structure and modified bases on the inhibition of trypanosomatid protein synthesis in cellfree extracts by antisense oligodeoxynucleotides

1990 • 25 citations

Evidence for sequence-specific conformational changes in DNA from the melting temperatures of DNA phosphorothioate derivatives

1985 • 23 citations

Circular dichroism studies of an oligo-α-thymidylate and of its interactions with complementary sequences

1988 • 23 citations

Uptake of polynucleotides by mammalian cells

1974 • 22 citations

Oxidative and nonoxidative formation of internucleotide linkages

1989 • 22 citations

Complementary oligonucleotide sequence inhibits both Vmw65 gene expression and replication of herpes simplex virus

1990 • 16 citations

Biological Activities of Oligonucleotides Linked to Poly(L-Lysine)

1987 • 14 citations

2′,3′ -Dideoxy-3′ -C-(phosphonomethyl)adenosine, the Phosphonate Analogue of 2′-Deoxyadenosine 3′-Phosphate

1983 • 13 citations

Embryology Gets Down—To the Molecular Level

1990 • 7 citations

Antisense Oligonucleotides as Anti-Viral Agents: New Drugs for a New Age

1991 • 2 citations