Characterization of Caspase Processing and Activation in HL-60 Cell Cytosol Under Cell-free Conditions

Measurement of protein using bicinchoninic acid

1985 • 17,516 citations

Cell Death: The Significance of Apoptosis

1980 • 7,281 citations

Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade

1997 • 7,177 citations

Caspases: Enemies Within

1998 • 6,923 citations

Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c

1996 • 5,133 citations

Apoptosis by Death Factor

1997 • 5,038 citations

Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked

1997 • 4,921 citations

Genistein, a specific inhibitor of tyrosine-specific protein kinases.

1987 • 3,773 citations

Apaf-1, a Human Protein Homologous to C. elegans CED-4, Participates in Cytochrome c–Dependent Activation of Caspase-3

1997 • 3,130 citations

Regulation of Cell Death Protease Caspase-9 by Phosphorylation

1998 • 3,129 citations

Mammalian Caspases: Structure, Activation, Substrates, and Functions During Apoptosis

1999 • 2,796 citations

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE

1994 • 2,575 citations

Ordering the Cytochrome c–initiated Caspase Cascade: Hierarchical Activation of Caspases-2, -3, -6, -7, -8, and -10 in a Caspase-9–dependent Manner

1999 • 1,927 citations

Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis.

1993 • 1,888 citations

Intracellular Adenosine Triphosphate (ATP) Concentration: A Switch in the Decision Between Apoptosis and Necrosis

1997 • 1,851 citations

Reduced Apoptosis and Cytochrome c–Mediated Caspase Activation in Mice Lacking Caspase 9

1998 • 1,642 citations

IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases

1998 • 1,394 citations

Apoptosis: Mechanisms and Roles in Pathology

1991 • 1,368 citations

The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases

1997 • 1,316 citations

Proteases to die for

1998 • 1,241 citations

Intracellular ATP levels determine cell death fate by apoptosis or necrosis.

1997 • 1,218 citations

Autoactivation of Procaspase-9 by Apaf-1-Mediated Oligomerization

1998 • 1,132 citations

Inhibition of Human Caspases by Peptide-based and Macromolecular Inhibitors

1998 • 984 citations

Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note.

1989 • 937 citations

Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle.

1977 • 835 citations

Distinct Caspase Cascades Are Initiated in Receptor-mediated and Chemical-induced Apoptosis

1999 • 829 citations

Mitochondrial Release of Caspase-2 and -9 during the Apoptotic Process

1999 • 730 citations

Direct Evidence for the Presence of Histidine in the Active Center of Chymotrypsin^*

1963 • 649 citations

A License to Kill

1996 • 649 citations

Sulfonyl Fluorides as Inhibitors of Esterases. I. Rates of Reaction with Acetylcholinesterase, α-Chymotrypsin, and Trypsin

1963 • 646 citations

Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.

1983 • 616 citations

Apoptosis and the Dilemma of Cancer Chemotherapy

1997 • 615 citations

Biochemical Characteristics of Caspases-3, -6, -7, and -8

1997 • 536 citations

Calcium‐activated DNA fragmentation kills immature thymocytes

1989 • 533 citations

Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.

1996 • 529 citations

Caspase-9 Can Be Activated without Proteolytic Processing

1999 • 498 citations

Zinc Is a Potent Inhibitor of the Apoptotic Protease, Caspase-3

1997 • 486 citations

Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32

1996 • 481 citations

Intracellular K+ Suppresses the Activation of Apoptosis in Lymphocytes

1997 • 458 citations

Mch3, a novel human apoptotic cysteine protease highly related to CPP32.

1995 • 391 citations

Cytochrome c activation of CPP32-like proteolysis plays a critical role in a Xenopus cell-free apoptosis system

1997 • 388 citations

Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells

1997 • 367 citations

Human cathepsin B1. Purification and some properties of the enzyme

1973 • 322 citations

PROTEOLYTIC ACTIVITIES THAT MEDIATE APOPTOSIS

1998 • 282 citations

Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation

1977 • 280 citations

Ubiquitin-lysozyme conjugates. Identification and characterization of an ATP-dependent protease from rabbit reticulocyte lysates.

1986 • 278 citations

Transducing signals of life and death

1997 • 275 citations

Inhibition of myosin ATPase by vanadate ion.

1979 • 269 citations

Comparison of Apoptosis in Wild-Type and Fas-Resistant Cells: Chemotherapy-Induced Apoptosis Is Not Dependent on Fas/Fas Ligand Interactions

1997 • 262 citations

A Sequential Two-Step Mechanism for the Production of the Mature p17:p12 Form of Caspase-3 in Vitro

1997 • 237 citations

Reaction of serine proteases with substituted isocoumarins: discovery of 3,4-dichloroisocoumarin, a new general mechanism based serine protease inhibitor

1985 • 231 citations

Activation of Multiple Interleukin-1β Converting Enzyme Homologues in Cytosol and Nuclei of HL-60 Cells during Etoposide-induced Apoptosis

1997 • 211 citations

Phosphotyrosyl-protein phosphatase of TCRC-2 cells.

1982 • 195 citations

Comparison of Apoptosis in Wild-Type and Fas-Resistant Cells: Chemotherapy-Induced Apoptosis Is Not Dependent on Fas/Fas Ligand Interactions

1997 • 189 citations

Activation of the CPP32 protease in apoptosis induced by 1-beta-D- arabinofuranosylcytosine and other DNA-damaging agents

1996 • 171 citations

Interleukin‐1βconverting enzyme: A novel cysteine protease required for IL‐1β production and implicated in programmed cell death

1995 • 170 citations

Caenorhabditis elegans CED-4 stimulates CED-3 processing and CED-3-induced

1997 • 168 citations

Pivotal role of a DEVD-sensitive step in etoposide-induced and Fas-mediated apoptotic pathways.

1996 • 167 citations

[40] Use of okadaic acid to inhibit protein phosphatases in intact cells

1991 • 162 citations

Comparison of Caspase Activation and Subcellular Localization in HL-60 and K562 Cells Undergoing Etoposide-Induced Apoptosis

1997 • 140 citations

Cysteinyl Proteinases and Their Selective Inactivation

1990 • 136 citations

Staurosporine is a potent inhibitor of p34cdc2 and p34cdc2-like kinases

1992 • 134 citations

Activation of caspases triggered by cytochrome c in vitro¹

1998 • 113 citations

Role of proteolysis in apoptosis: involvement of serine proteases in internucleosomal DNA fragmentation in immature thymocytes

1993 • 109 citations

Affinity labeling displays the stepwise activation of ICE-related proteases by Fas, staurosporine, and CrmA-sensitive caspase-8

1997 • 107 citations

Camptothecin-induced apoptosis in p53-null human leukemia HL60 cells and their isolated nuclei: effects of the protease inhibitors Z-VAD-fmk and dichloroisocoumarin suggest an involvement of both caspases and serine proteases

1997 • 107 citations

Comparison of Caspase Activation and Subcellular Localization in HL-60 and K562 Cells Undergoing Etoposide-Induced Apoptosis

1997 • 106 citations

Comparison of Paclitaxel-, 5-Fluoro-2′-deoxyuridine-, and Epidermal Growth Factor (EGF)-induced Apoptosis

1999 • 88 citations

Differential suppression by protease inhibitors and cytokines of apoptosis induced by wild-type p53 and cytotoxic agents.

1996 • 86 citations

Serine protease inhibitor TPCK prevents Taxol-induced cell death and blocks c-Raf-1 and Bcl-2 phosphorylation in human breast carcinoma cells

1999 • 73 citations

Inhibitors of Trypsin‐Like Serine Proteases Inhibit Processing of the Caspase Nedd‐2 and Protect PC12 Cells and Sympathetic Neurons from Death Evoked by Withdrawal of Trophic Support

1997 • 62 citations

Adenine nucleotides directly stimulate pertussis toxin.

1985 • 61 citations

Interactions of Chaperone α-Crystallin with the Molten Globule State of Xylose Reductase

1998 • 55 citations

A pre‐existing protease is a common effector of thymocyte apoptosis mediated by diverse stimuli

1995 • 53 citations

Phosphorylated Forms of Activated Caspases Are Present in Cytosol From HL-60 Cells During Etoposide-Induced Apoptosis

1998 • 47 citations

Protease Activity of in Vitro Transcribed and Translated Caenorhabditis elegans Cell Death Gene (ced-3) Product

1996 • 44 citations

Uncoupling ubiquitin-protein conjugation from ubiquitin-dependent proteolysis by use of .beta.,.gamma.-nonhydrolyzable ATP analogs

1991 • 43 citations

The DNA-binding properties of polyomavirus large T antigen are altered by ATP and other nucleotides

1991 • 42 citations

Metabolic effects and kill of human T-cell leukemia by 5-deazaacyclotetrahydrofolate, a specific inhibitor of glycineamide ribonucleotide transformylase.

1992 • 37 citations

Distinct steps in DNA fragmentation pathway during camptothecin-induced apoptosis involved caspase-, benzyloxycarbonyl- and N-tosyl-L-phenylalanylchloromethyl ketone-sensitive activities.

1998 • 37 citations

Zinc inhibits apoptosis upstream of ICE/CED-3 proteases rather than at the level of an endonuclease

1997 • 35 citations

Aurintricarboxylic acid is an inhibitor of mu- and m-calpain.

1995 • 35 citations

Deoxynucleoside Induces Neuronal Apoptosis Independent of Neurotrophic Factors

1995 • 33 citations

ATP binding causes a conformational change in the .gamma. subunit of the Escherichia coli F1ATPase which is reversed on bond cleavage

1994 • 29 citations

Binding of ATP to eukaryotic initiation factor 2. Differential modulation of mRNA-binding activity and GTP-dependent binding of methionyl-tRNAMetf.

1990 • 29 citations

Hydrolysis of AMPPNP by the motor domain of ncd, a kinesin‐related protein

1997 • 18 citations

Absence of a phosphorylated intermediate during ATP hydrolysis by Escherichia coli transcription termination protein rho.

1986 • 16 citations

Phosphorylated Forms of Activated Caspases Are Present in Cytosol From HL-60 Cells During Etoposide-Induced Apoptosis

1998 • 15 citations

Decrease of cellular ATP by dihexanoylglycerol may limit responses to protein kinase C activation

1987 • 10 citations

Adenosine 5'-O-(3-thiotriphosphate) hydrolysis by dynein

1989 • 10 citations

Hydrolysis of adenylyl imidodiphosphate in the presence of Na+ + Mg+ by (Na+ + K+)-activated ATPase

1983 • 9 citations

Evidence for a relationship between intracellular GTP levels and the induction of HL-60 leukemia cell differentiation by 5,10-dideazatetrahydrofolic acid (DDATHF).

1993 • 4 citations