Efficient secretion and purification of human insulin-like growth factor I with a gene fusion vector inStaphylococci

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.

1978 • 1,530 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin

1977 • 829 citations

Expression in Escherichia coli of chemically synthesized genes for human insulin.

1979 • 813 citations

Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance

1982 • 744 citations

Insulin-like growth factor I stimulates growth in hypophysectomized rats

1982 • 605 citations

Insulin‐Like Growth Factors I and II: Some Biological Actions and Receptor Binding Characteristics of Two Purified Constituents of Nonsuppressible Insulin‐Like Activity of Human Serum

1978 • 384 citations

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

1984 • 374 citations

[15] Cleavage at aspartylprolyl bonds

1977 • 319 citations

Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.

1984 • 159 citations

Secretion of Interferon by Bacillus subtilis

1983 • 150 citations

Gene fusion vectors based on the gene for staphylococcal protein A

1983 • 137 citations

Somatomedin-C and platelet-derived growth factor stimulate human fibroblast replication

1981 • 111 citations

RADIORECEPTOR ASSAY FOR SOMATOMEDIN A¹

1974 • 76 citations

Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative staphylococci

1984 • 73 citations

Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent φX174 mutant, with a specific nucleotide deletion, at very high efficiency

1980 • 57 citations

Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.

1981 • 52 citations

A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.

1982 • 37 citations

Effects of streptomycin and novobiocin on Staphylococcus aureus gene expression

1978 • 16 citations