A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

A system for shotgun DNA sequencing

1981 • 2,710 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants

1981 • 776 citations

Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event

1970 • 770 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

1978 • 282 citations

Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.

1980 • 195 citations

Nucleic Acid Reactivity and Conformation

1973 • 170 citations

Uracil incorporation: A source of pulse-labeled DNA fragments in the replication of the Escherichia coli chromosome

1978 • 151 citations

Regulatory mutants of simian virus 40: Constructed mutants with base substitutions at the origin of DNA replication

1979 • 131 citations

Cold-sensitive regulatory mutants of simian virus 40

1980 • 130 citations

Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.

1980 • 123 citations

Site-directed mutagenesis in DNA: Generation of point mutations in cloned β globin complementary DNA at the positions corresponding to amino acids 121 to 123

1978 • 78 citations

Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule.

1980 • 67 citations

The effect of bisulfite modification on the template activity of DNA for DNA polymerase I¹.

1974 • 25 citations

Induction and isolation of mutants in a specific region of gene A of bacteriophage ∅X174

1976 • 20 citations

Region- and strand-specific mutagenesis of a recombinant plasmid

1981 • 17 citations

Annual review of genetics

1987 • 15 citations