RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age

Initial sequencing and analysis of the human genome

2001 • 23,520 citations

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

1998 • 14,674 citations

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

2001 • 9,275 citations

RNA interference

2002 • 3,369 citations

Genome evolution in yeasts

2004 • 1,579 citations

Fundamentals of experimental design for cDNA microarrays

2002 • 1,070 citations

A large-scale RNAi screen in human cells identifies new components of the p53 pathway

2004 • 1,057 citations

Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-κB

2003 • 946 citations

Microarrays of cells expressing defined cDNAs

2001 • 920 citations

Identification of essential genes in cultured mammalian cells using small interfering RNAs

2001 • 835 citations

Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways

2000 • 832 citations

Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

2004 • 699 citations

A resource for large-scale RNA-interference-based screens in mammals

2004 • 685 citations

RNAi in C. elegans : Soaking in the Genome Sequence

1998 • 635 citations

The art and design of genetic screens: Drosophila melanogaster

2002 • 625 citations

Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar–coding strategy

1996 • 570 citations

Post-analysis follow-up and validation of microarray experiments

2002 • 444 citations

Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.

1991 • 442 citations

The art and design of genetic screens: Caenorhabditis elegans

2002 • 421 citations

Identification of Modulators of TRAIL-Induced Apoptosis via RNAi-Based Phenotypic Screening

2003 • 343 citations

Apoptotic Molecular Machinery: Vastly Increased Complexity in Vertebrates Revealed by Genome Comparisons

2001 • 321 citations

The art and design of genetic screens: yeast

2001 • 272 citations

Structural requirements of double-stranded RNA for the activation of 2‘,5‘-oligo(A) polymerase and protein kinase of interferon-treated HeLa cells.

1979 • 263 citations

Molecular Dissection of Cytokinesis by RNA Interference inDrosophilaCultured Cells

2002 • 232 citations

A library of siRNA duplexes targeting the phosphoinositide 3-kinase pathway: determinants of gene silencing for use in cell-based screens

2004 • 226 citations

RNA interference: the new somatic cell genetics?

2002 • 218 citations

RNAi Microarray Analysis in Cultured Mammalian Cells

2003 • 187 citations

High-Throughput Selection of Effective RNAi Probes for Gene Silencing

2003 • 169 citations

RNA interference microarrays: High-throughput loss-of-function genetics in mammalian cells

2004 • 166 citations

Use of a Screen for Synthetic Lethal and Multicopy Suppressee Mutants To Identify Two New Genes Involved in Morphogenesis in Saccharomyces cerevisiae

1991 • 166 citations

Interactions between Double-Stranded RNA Regulators and the Protein Kinase DAI

1992 • 151 citations

Identification of p53 regulators by genome-wide functional analysis

2004 • 142 citations

The art and design of genetic screens: Escherichia coli

2003 • 96 citations

Experiments using microarray technology: limitations and standard operating procedures

2003 • 86 citations

Arrayed adenoviral expression libraries for functional screening

2002 • 85 citations

RNA Interference

2004 • 55 citations

Identifying Mutations in Duplicated Functions in Saccharomyces cerevisiae: Recessive Mutations in HMG-CoA Reductase Genes

1987 • 49 citations

Retroviral insertional mutagenesis: Tagging cancer pathways

2003 • 39 citations

Genetic Changes in Human Populations, Especially Those Due to Gene Flow and Genetic Drift

1954 • 28 citations

Building a protein interaction map: research in the post-genome era

2000 • 7 citations