Design of high-throughput methods of protein production for structural biology

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Green Fluorescent Protein as a Marker for Gene Expression

1994 • 6,572 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Dynamic Light Scattering

1985 • 4,912 citations

A generic protein purification method for protein complex characterization and proteome exploration

1999 • 2,699 citations

Strategies for achieving high-level expression of genes in Escherichia coli

1996 • 1,099 citations

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1988 • 1,030 citations

Preparation and analysis of protein crystals

1982 • 976 citations

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

1988 • 934 citations

A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

1993 • 897 citations

Rapid protein-folding assay using green fluorescent protein

1999 • 879 citations

GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli

1989 • 703 citations

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1988 • 662 citations

In vitro folding of inclusion body proteins

1996 • 659 citations

Strategies for optimizing heterologous protein expression in Escherichia coli

1998 • 486 citations

Cell-free production and stable-isotope labeling of milligram quantities of proteins

1999 • 485 citations

Advances in refolding of proteins produced in E. coli

1998 • 476 citations

Refolding of recombinant proteins

1998 • 472 citations

Metal-Affinity Separations: A New Dimension in Protein Processing

1991 • 455 citations

Crystallization of membrane proteins

1997 • 444 citations

Production of Soluble Recombinant Proteins in Bacteria

1989 • 430 citations

Protein Folding Intermediates and Inclusion Body Formation.

1989 • 423 citations

Crystallization of Nucleic Acids and Proteins: A Practical Approach

1992 • 393 citations

Preparation and analysis of protein crystals

1982 • 366 citations

Mass spectrometry

2000 • 355 citations

Expression of correctly folded proteins in Escherichia coli

1996 • 332 citations

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.

1985 • 331 citations

Affinity Fusion Strategies for Detection, Purification, and Immobilization of Recombinant Proteins

1997 • 330 citations

Protein identification methods in proteomics

2000 • 324 citations

Secretion cloning vectors in Escherichia coli.

1984 • 322 citations

New fusion protein systems designed to give soluble expression in Escherichia coli

1999 • 321 citations

Crystallization of Nucleic Acids and Proteins

1999 • 316 citations

New fusion protein systems designed to give soluble expression inEscherichia coli

1999 • 311 citations

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment

1993 • 288 citations

The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes

1998 • 279 citations

Structure and Morphology of Protein Inclusion Bodies in Escherichia Coli

1991 • 238 citations

6xHis-Ni-NTA Chromatography as a Superior Technique in Recombinant Protein Expressiod/Purification

2003 • 223 citations

Gene fusion expression systems in Escherichia coli

1995 • 222 citations

Protein Misfolding and Inclusion Body Formation in Recombinant Escherichia coli Cells Overexpressing Heat-shock Proteins

1996 • 222 citations

A novel strategy for production of a highly expressed recombinant protein in an active form

1991 • 218 citations

Building bridges: disulphide bond formation in the cell

1994 • 213 citations

Ribonuclease S‐peptide as a carrier in fusion proteins

1993 • 193 citations

Protein folding in the periplasm of Escherichia coli

1994 • 175 citations

Proteomics: Capacityversus utility

2000 • 163 citations

Crystallization of nucleic acids and proteins. A practical approach

1993 • 153 citations

Fusion tails for the recovery and purification of recombinant proteins

1991 • 146 citations

[12] Gene fusions for purpose of expression: An introduction

1990 • 145 citations

A Human cDNA Library for High-Throughput Protein Expression Screening

2000 • 140 citations

Construction and Characterization of a Set of E. coli Strains Deficient in All Known Loci Affecting the Proteolytic Stability of Secreted Recombinant Proteins

1994 • 140 citations

Escherichia coliSkp Chaperone Coexpression Improves Solubility and Phage Display of Single-Chain Antibody Fragments

1999 • 134 citations

Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution.

2000 • 132 citations

Crystal Structure of Tyrosine Hydroxylase with Bound Cofactor Analogue and Iron at 2.3 Å Resolution: Self-Hydroxylation of Phe300 and the Pterin-Binding Site^,

1998 • 131 citations

Use of dynamic light scattering to assess crystallizability of macromolecules and macromolecular assemblies

1994 • 131 citations

[20] Solubilization of protein aggregates

1990 • 127 citations

Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli.

1992 • 121 citations

Protein Crystallization: Techniques, Strategies, and Tips.

1999 • 119 citations

Higher Throughput Bioanalysis by Automation of a Protein Precipitation Assay Using a 96-Well Format with Detection by LC−MS/MS

2000 • 117 citations

A new protein folding screen: Application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase

1999 • 111 citations

Solubility of Proteins Isolated from Inclusion Bodies Is Enhanced by Fusion to Maltose-Binding Protein or Thioredoxin

1998 • 108 citations

High-performance hydrophobic interaction chromatography as a tool for protein refolding

1992 • 105 citations

Overcoming Inclusion Body Formation in a High-Level Expression System

1993 • 103 citations

Renaturation of citrate synthase: Influence of denaturant and folding assistants

1992 • 103 citations

Gene expression systems : using nature for the art of expression

1999 • 103 citations

[12] Purification of overproduced Escherichia coli RNA polymerase σ factors by solubilizing inclusion bodies and refolding from Sarkosyl

1996 • 101 citations

Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli

1995 • 97 citations

Inclusion bodies and purification of proteins in biologically active forms

1997 • 92 citations

[10] Dynamic light scattering in evaluating crystallizability of macromolecules

1997 • 85 citations

Tandem Immobilized Metal-Ion Affinity Chromatography/Immunoaffinity Purification of His-tagged Proteins— Evaluation of Two Anti-His-Tag Monoclonal Antibodies

1998 • 84 citations

Enzyme Immobilization Using the Cellulose-Binding Domain of a Cellulomonas Fimi Exoglucanase

1989 • 81 citations

Crystallizing proteins – a rational approach?

1994 • 79 citations

Chaperonins groEL and groES promote assembly of heterotetramers (alpha 2 beta 2) of mammalian mitochondrial branched-chain alpha-keto acid decarboxylase in Escherichia coli.

1992 • 77 citations

Correctly Folded T-cell Receptor Fragments in the Periplasm of Escherichia coli

1994 • 76 citations

Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies

1995 • 73 citations

Histidine Patch Thioredoxins

1996 • 70 citations

Microdrop screening: a rapid method to optimize solvent conditions for NMR spectroscopy of proteins.

1998 • 62 citations

Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose

1989 • 61 citations

Active papain renatured and processed from insoluble recombinant propapain expressed in Escherichia coli

1992 • 46 citations

Cellulose-binding domains: potential for purification of complex proteins

1992 • 43 citations

Fusion proteins in biotechnology and structural biology

1992 • 34 citations

Protein Expression Strategies for Identification of Novel Target Proteins

2000 • 26 citations

Enzymatic and Chemical Cleavage of Fusion Proteins

1994 • 26 citations

Fusion proteins in biotechnology and structural biology

1992 • 25 citations

Expression in Escherichia coli: Purification and properties of the yeast general transcription factor TFIID

1991 • 25 citations

Peptide Tags for a Dual Affinity Fusion System

1994 • 25 citations

An Ultrahigh-Throughput Screening Assay for Estrogen Receptor Ligands

1999 • 24 citations

Protein fusions: bioseparation and application

1991 • 22 citations

Refolding, Purification, and Characterization of a Loop Deletion Mutant of Human Bcl-2 from Bacterial Inclusion Bodies

1999 • 21 citations

Introduction to Expression by Fusion Protein Vectors

1994 • 15 citations

Array-based proteomics: high-throughput expression and purification of IMAGE consortium cDNA clones.

1999 • 13 citations

Tandem Affinity Tags for the Purification of Bivalent Anti-DNA Single-Chain Fv Expressed in Escherichia coli

1999 • 12 citations

[Large-scale protein preparation using the cell-free synthesis].

1999 • 11 citations

An Introduction to the Crystallogenesis of Biological Macromolecules

1999 • 11 citations

Expression Using Vectors with Phage λ Regulatory Sequences

1990 • 8 citations

Molecular Biology for Structural Biology

1999 • 6 citations