In vitro folding of inclusion body proteins

MOLECULAR CHAPERONE FUNCTIONS OF HEAT-SHOCK PROTEINS

1993 • 1,591 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1987 • 1,107 citations

Assembly of a Functional Immunoglobulin F _v Fragment in Escherichia coli

1988 • 973 citations

Identification of a protein required for disulfide bond formation in vivo

1991 • 967 citations

Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding

1992 • 965 citations

The purification of eukaryotic polypeptides synthesized in Escherichia coli

1986 • 838 citations

Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin

1977 • 829 citations

Reconstitution of active dimeric ribulose bisphosphate carboxylase from an unfolded state depends on two chaperonin proteins and Mg-ATP

1989 • 737 citations

Folding and association of proteins

1987 • 697 citations

GroE facilitates refolding of citrate synthase by suppressing aggregation

1991 • 486 citations

Dynamics of the Chaperonin ATPase Cycle: Implications for Facilitated Protein Folding

1994 • 461 citations

Supervising the fold: functional principles of molecular chaperones

1996 • 420 citations

Formation of Soluble Recombinant Proteins in Escherichia Coli is Favored by Lower Growth Temperature

1988 • 418 citations

Renaturation, Purification and Characterization of Recombinant Fab-Fragments Produced in Escherichia coli

1991 • 385 citations

Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli

1992 • 299 citations

Solubility as a Function of Protein Structure and Solvent Components

1990 • 295 citations

Reconstitution of lactic dehydrogenase. Noncovalent aggregation vs. reactivation. 1. Physical properties and kinetics of aggregation

1979 • 282 citations

Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca2+ and heme.

1990 • 280 citations

Reductive Cleavage of Disulfide Bridges in Ribonuclease

1957 • 278 citations

Formation of recombinant protein inclusion bodies in Escherichia coli

1988 • 253 citations

Predicting the Solubility of Recombinant Proteins in Escherichia coli

2014 • 249 citations

Artificial Chaperones: Protein Refolding via Sequential Use of Detergent and Cyclodextrin

1995 • 231 citations

Native-like Secondary Structure in Interleukin-1.beta. Inclusion Bodies by Attenuated Total Reflectance FTIR

1994 • 203 citations

Identification and characterization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli.

1995 • 190 citations

Increased thermal stability of proteins in the presence of amino acids

1994 • 162 citations

Polyethylene glycol enhanced refolding of bovine carbonic anhydrase B. Reaction stoichiometry and refolding model.

1992 • 160 citations

Nonenzymic reactivation of reduced bovine pancreatic ribonuclease by air oxidation and by glutathione oxidoreduction buffers.

1975 • 159 citations

Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase

1983 • 158 citations

On the cysteine and cystine content of proteins

1977 • 153 citations

Improved Refolding of an Immobilized Fusion Protein

1996 • 148 citations

Examination of calf prochymosin accumulation in Escherichia coli: disulphide linkages are a structural component of prochymosin-containing inclusion bodies.

1985 • 139 citations

Size and Density of Protein Inclusion Bodies

1986 • 135 citations

Detergent-assisted refolding of guanidinium chloride-denatured rhodanese. The effects of the concentration and type of detergent.

1987 • 131 citations

Secondary structure characterization of ß-lactamase inclusion bodies

1994 • 128 citations

The renaturation of reduced chymotrypsinogen A in guanidine HCl. Refolding versus aggregation.

1978 • 125 citations

Control of formation of active soluble or inactive insoluble baker's yeast α-glucosidase PI in Escherichia coli by induction and growth conditions

1989 • 114 citations

Effect of cyclodextrins on the thermal stability of globular proteins

1992 • 111 citations

Refolding proteins by gel filtration chromatography

1994 • 105 citations

Large Scale, In Situ Isolation of Periplasmic IGF–I from E. coli

1994 • 101 citations

Independent domain folding of Pseudomonas exotoxin and single-chain immunotoxins: influence of interdomain connections.

1992 • 91 citations

Protein refolding in reversed micelles

1990 • 90 citations

Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coli

1989 • 82 citations

Protein composition of Vitreoscilla hemoglobin inclusion bodies produced in Escherichia coli.

1990 • 72 citations

A novel sequential procedure to enhance the renaturation of recombinant protein from Escherichia coil inclusion bodies

1992 • 72 citations

The oxidative folding of proteins by disulfide plus thiol does not correlate with redox potential

1987 • 68 citations

Facilitated In Vitro Refolding of Human Recombinant Insulin-Like Growth Factor I Using a Solubilizing Fusion Partner

1991 • 68 citations

Stabilization of creatinase from Pseudomonas putida by random mutagenesis

1993 • 58 citations

Effect of polyanions on the refolding of human acidic fibroblast growth factor.

1991 • 50 citations

Antibody Assisted Protein Refolding

1992 • 49 citations

Comparing the Refolding and Reoxidation of Recombinant Porcine Growth Hormone from a Urea Denatured State and from Escherichia coli Inclusion Bodies

1995 • 49 citations

Expression in escherichia coli of a chemically synthesized gene for a “mini-c” analog of human proinsulin

1981 • 47 citations

Renaturation of Casein Kinase II from Recombinant Subunits Produced in Escherichia coli: Purification and Characterization of the Reconstituted Holoenzyme

1993 • 43 citations

Expression and Folding of Recombinant Bovine Prethrombin-2 and Its Activation to Thrombin

1995 • 41 citations

Protein Refolding at High Concentrations Using Detergent/Phospholipid Mixtures

1994 • 41 citations

Dimerization and reactivation of triosephosphate isomerase in reverse micelles

1992 • 37 citations

Protein refolding using aqueous two-phase systems

1994 • 23 citations

Isolation and purification of a biologically active human platelet-derived growth factor BB expressed in Escherichia coli

1992 • 15 citations