Strategies for optimizing heterologous protein expression in Escherichia coli

Amino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis

1986 • 2,566 citations

Strategies for achieving high-level expression of genes in Escherichia coli

1996 • 1,099 citations

High cell-density culture of Escherichia coli

1996 • 847 citations

The Role of the Thioredoxin and Glutaredoxin Pathways in Reducing Protein Disulfide Bonds in the Escherichia coliCytoplasm

1997 • 637 citations

The N-End Rule in Bacteria

1991 • 536 citations

High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product

1989 • 363 citations

Expression of correctly folded proteins in Escherichia coli

1996 • 332 citations

Increase of Solubility of Foreign Proteins in Escherichia coli by Coproduction of the Bacterial Thioredoxin

1995 • 319 citations

The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli.

1995 • 269 citations

Recombinant Protein Expression in High Cell Density Fed-Batch Cultures of Escherichia Coli

1992 • 254 citations

Predicting the Solubility of Recombinant Proteins in Escherichia coli

2014 • 249 citations

Gene fusion expression systems in Escherichia coli

1995 • 222 citations

Chaperone-assisted protein folding

1997 • 180 citations

Protein engineering : principles and practice

1996 • 116 citations

Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria – a review

1997 • 106 citations

Upstream Strategies to Minimize Proteolytic Degradation upon Recombinant Production inEscherichia coli

1996 • 100 citations

A soluble, single-chain T-cell receptor fragment endowed with antigen-combining properties.

1991 • 97 citations

Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli

1995 • 97 citations

Protein expression using ubiquitin fusion and cleavage

1996 • 87 citations

Eukaryotic Protein Disulfide Isomerase Complements Escherichia coli dsbA Mutants and Increases the Yield of a Heterologous Secreted Protein with Disulfide Bonds

1996 • 86 citations

Correctly Folded T-cell Receptor Fragments in the Periplasm of Escherichia coli

1994 • 76 citations

[15] Engineering Escherchia coli to secrete heterologous gene products

1990 • 65 citations

Human Protein Disulfide Isomerase Functionally Complements a dsbA Mutation and Enhances the Yield of Pectate Lyase C in Escherichia coli

1995 • 60 citations

Chaperone-assisted protein expression

1996 • 59 citations

High-Level Expression of Human Thymidylate Synthase

1997 • 59 citations

Secretion of recombinant proteins by Gram-negative bacteria

1996 • 52 citations

Kinetic Studies for the Optimization of Recombinant Protein Formationa

1996 • 32 citations

A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor Vβ5.3 in Escherichia coli

1996 • 31 citations

Construction of lacIts and lacIqts expression plasmids and evaluation of the thermosensitive lac repressor

1995 • 27 citations

Expression of the Human T-Cell Receptor Vβ5.3 inEscherichia coliby Thermal Induction of thetrcPromoter: Nucleotide Sequence of thelacItsGene

1995 • 10 citations