Construction and Characterization of a Set of E. coli Strains Deficient in All Known Loci Affecting the Proteolytic Stability of Secreted Recombinant Proteins

STRUCTURE AND FUNCTION OF BACTERIAL SIGMA FACTORS

1988 • 990 citations

Selection for loss of tetracycline resistance by Escherichia coli

1981 • 585 citations

Heat Shock Response

1991 • 576 citations

Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genes

1985 • 521 citations

Proteases and protein degradation inEscherichia coli

1992 • 387 citations

Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature

1989 • 375 citations

An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins.

1988 • 374 citations

Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures

1989 • 342 citations

Sequence analysis and regulation of thehtrA gene ofEscherichin coli:a σ³²-independent mechanism of heat-inducible transcription

1988 • 259 citations

Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli.

1989 • 250 citations

Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32

1988 • 219 citations

Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli.

1984 • 217 citations

DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli.

1992 • 214 citations

Escherichia coli heat shock gene mutants are defective in proteolysis.

1988 • 205 citations

Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C termini.

1992 • 199 citations

A gene regulating the heat shock response in Escherichia coli also affects proteolysis.

1984 • 171 citations

Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3

1991 • 163 citations

Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.

1984 • 159 citations

8 The Function and Regulation of Heat Shock Proteins in Escherichia coli

1990 • 103 citations

Construction and characterization of Escherichia coli strains deficient in multiple secreted proteases: protease III degrades high-molecular-weight substrates in vivo

1991 • 101 citations

Control of in vivo proteolysis in the production of recombinant proteins

1992 • 90 citations

A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12

1975 • 79 citations

[15] Engineering Escherchia coli to secrete heterologous gene products

1990 • 65 citations

Peptidases and proteases of Escherichia coli and Salmonella typhimurium

1989 • 61 citations

Expression, purification, and enzymatic characterization of a protein A-β-lactamase hybrid protein

1989 • 22 citations

Independence of bacteriophage N15 lytic and linear plasmid replication from the heat shock proteins DnaJ, DnaK, and GrpE

1991 • 13 citations

Peptidases and proteases of Escherichia coli and Salmonella typhimurium

1989 • 13 citations

High‐Level Production of Proteolytically Sensitive Secreted Proteins in Escherichia coli Strains Impaired in the Heat‐Shock Response^a

1994 • 7 citations