Cis preference of the IS 903 transposase is mediated by a combination of transposase instability and inefficient translation

Experiments in molecular genetics

1972 • 28,260 citations

Amino Acid Preferences for Specific Locations at the Ends of α Helices

1988 • 1,356 citations

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.

1987 • 738 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

[6] Efficient site-directed mutagenesis using uracil-containing DNA

1991 • 644 citations

Translation initiation in Escherichia coli: sequences within the ribosome‐binding site

1992 • 344 citations

IS10 transposition is regulated by DNA adenine methylation

1985 • 341 citations

The 3′-5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.

1991 • 279 citations

Cloning of replication, incompatibility, and stability functions of R plasmid NR1

1980 • 154 citations

Translational efficiency of the Escherichia coli adenylate cyclase gene: mutating the UUG initiation codon to GUG or AUG results in increased gene expression.

1985 • 143 citations

Tn10 transposase acts preferentially on nearby transposon ends in vivo

1983 • 138 citations

Regulation of Transposition in Bacteria

1990 • 98 citations

Analysis of the Structure and Function of the Kanamycin-resistance Transposon Tn903

1981 • 94 citations

Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.

1983 • 93 citations

Amino acid substitutions that increase the thermal stability of the λ Cro protein

1989 • 91 citations

Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats.

1987 • 90 citations

Cis -Limited Action of the Gene-A Product of Bacteriophage ϕX174 and the Essential Bacterial Site

1972 • 75 citations

Insertion Sequence Duplication in Transpositional Recombination

1983 • 73 citations

Role of instability in the cis action of the insertion sequence IS903 transposase.

1990 • 70 citations

Evidence that the cis preference of the Tn5 transposase is caused by nonproductive multimerization.

1994 • 69 citations

Replicon fusions promoted by the inverted repeats of Tn5

1981 • 68 citations

In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.

1984 • 68 citations

Factors determining frequency of plasmid cointegration mediated by insertion sequence IS 1

1982 • 67 citations

Tn10 protects itself at two levels from fortuitous activation by external promoters

1985 • 67 citations

Binding of the IS903 transposase to its inverted repeat in vitro.

1992 • 60 citations

Use of a Tn5 derivative that creates lacZ translational fusions to obtain a transposition mutant

1988 • 49 citations

A New Generalizable Test for Detection of Mutations Affecting Tn10 Transposition

1987 • 48 citations

ON THE NATURE OF CIS-ACTING REGULATORY PROTEINS AND GENETIC ORGANIZATION IN BACTERIOPHAGE: THE EXAMPLE OF GENE Q OF BACTERIOPHAGE Λ

1976 • 47 citations

Selection of lac gene fusions in vivo: ompR-lacZ fusions that define a functional domain of the ompR gene product

1984 • 44 citations

IS 10 mRNA stability and steady state levels in Escherichia coli: indirect effects of translation and role of rne function

1993 • 42 citations

cis-acting proteins

1986 • 38 citations

A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro

1988 • 37 citations

Preferential cis action of IS 10 transposase depends upon its mode of synthesis

1993 • 34 citations

RepA protein- andoriR-dependent initiation of R1 plasmid replication: identification of arho-dependent transcription terminator required forcis-action ofrepA protein

1988 • 32 citations

Translation initiation of IS50R read-through transcripts

1991 • 30 citations

An essential proline in .lambda. repressor is required for resistance to intracellular proteolysis

1990 • 28 citations

Stoichiometric use of the transposase of bacteriophage Mu

1984 • 27 citations

The effect of chloramphenicol on synthesis of ΦX 174-specific proteins and detection of the cistron A protein

1972 • 20 citations

Instability of bacteriophage Mu transposase and the role of host Hfl protein

1990 • 10 citations

An IS903-based vector for transposon mutagenesis and the isolation of gene fusions

1995 • 10 citations