The dacA gene of Bacillus stearothermophilus coding for d-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A general method applicable to the search for similarities in the amino acid sequence of two proteins

1970 • 11,049 citations

Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology

1987 • 4,385 citations

Escherichia coli and Salmonella typhimurium: Cellular and molecular biology

1988 • 3,957 citations

A gas-liquid solid phase peptide and protein sequenator.

1981 • 2,238 citations

The hydrophobic moment detects periodicity in protein hydrophobicity.

1984 • 877 citations

[12] High-efficiency transformation of yeast by electroporation

1991 • 798 citations

The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 <scp>dd</scp>-peptidase family

1988 • 334 citations

Yeast Systems for the Commercial Production of Heterologous Proteins

1991 • 314 citations

A Comprehensive Molecular Map of Bacteriophage LAMBDA

1979 • 277 citations

In vivo and in vitro transcription of the Clostridium pasteurianum ferredoxin gene. Evidence for "extended" promoter elements in gram-positive organisms.

1986 • 252 citations

Transformation in Escherichia coli: cryogenic preservation of competent cells

1977 • 242 citations

The mechanism of action of penicillin. Penicillin acylates the active site of Bacillus stearothermophilus D-alanine carboxypeptidase.

1980 • 176 citations

β‐Lactamase of Bacillus licheniformis 749/C at 2 Å resolution

1990 • 175 citations

Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli

1985 • 174 citations

Purification to homogeneity and properties of two D-alanine carboxypeptidases I From Escherichia coli.

1976 • 147 citations

Structural comparison of the Pichia pastoris alcohol oxidase genes

1989 • 143 citations

Crystallographic mapping of β-lactams bound to a d-alanyl-d-alanine peptidase target enzyme

1989 • 105 citations

Spherical E. coli due to elevated levels of D-alanine carboxypeptidase

1982 • 101 citations

High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris

1988 • 94 citations

Penicillin Target Enzyme and the Antibiotic Binding Site

1982 • 81 citations

Isolation of a mutant of Escherichia coli lacking penicillin-sensitive D-alanine carboxypeptidase IA.

1978 • 81 citations

Hydrophobicity and amphiphilicity in protein structure

1986 • 80 citations

Reduced heat resistance of mutant spores after cloning and mutagenesis of the Bacillus subtilis gene encoding penicillin-binding protein 5

1986 • 69 citations

The C terminus of penicillin-binding protein 5 is essential for localisation to the E. coli inner membrane.

1986 • 66 citations

[34] α-Factor leader-directed secretion of heterologous proteins from yeast

1990 • 57 citations

Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis

1992 • 56 citations

3′ Terminal sequences of 16S rRNA do not explain translational specificity differences between E. coli and B. stearothermophilus ribosomes

1977 • 50 citations

Active-site-serine <scp>d</scp>-alanyl-<scp>d</scp>-alanine-cleaving-peptidase-catalysed acyl-transfer reactions. Procedures for studying the penicillin-binding proteins of bacterial plasma membranes

1986 • 47 citations

The 47-kDa major lipoprotein immunogen of Treponema pallidum is a penicillin-binding protein with carboxypeptidase activity.

1994 • 44 citations

Cleavage of a COOH-terminal hydrophobic region from D-alanine carboxypeptidase, a penicillin-sensitive bacterial membrane enzyme. Characterization of active, water-soluble fragments.

1979 • 38 citations

Primary structure of the COOH-terminal membranous segment of a penicillin-sensitive enzyme purified from two Bacilli.

1981 • 36 citations

Mutations of the α-galactosidase signal peptide which greatly enhance secretion of heterologous proteins by yeast

1991 • 17 citations

Streptomyces R61 dd-carboxypeptidase: Hydrolysis of X-d-alanyl-d-alanine peptides measured by a fluorometric assay

1984 • 11 citations