High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii

2007 • 11,720 citations

An endotoxin-induced serum factor that causes necrosis of tumors.

1975 • 4,522 citations

Shock and Tissue Injury Induced by Recombinant Human Cachectin

1986 • 2,587 citations

Transformation of yeast.

1978 • 2,267 citations

Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin

1984 • 1,738 citations

Construction of Biologically Functional Bacterial Plasmids In Vitro

1973 • 1,699 citations

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels

1977 • 1,607 citations

Recombinant Human Tumor Necrosis Factor-α: Effects on Proliferation of Normal and Transformed Cells in Vitro

1985 • 1,596 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

Human tumor necrosis factor. Production, purification, and characterization.

1985 • 922 citations

Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors.

1985 • 922 citations

Tumour necrosis factor as immunomodulator and mediator of monocyte cytotoxicity induced by itself, γ-interferon and interleukin-1

1986 • 781 citations

Tumour necrosis factor α stimulates resorption and inhibits synthesis of proteoglycan in cartilage

1986 • 742 citations

Tumour necrosis factors α and β inhibit virus replication and synergize with interferons

1986 • 726 citations

Molecular Cloning of the Complementary DNA for Human Tumor Necrosis Factor

1985 • 633 citations

Cachectin/tumor necrosis factor regulates hepatic acute-phase gene expression.

1986 • 585 citations

Induction of β2-interferon by tumor necrosis factor: A homeostatic mechanism in the control of cell proliferation

1986 • 577 citations

Recombinant human TNF induces production of granulocyte–monocyte colony-stimulating factor

1986 • 528 citations

Human Lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization

1985 • 509 citations

Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro.

1986 • 496 citations

Pichia pastoris as a Host System for Transformations

1985 • 495 citations

Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor

1985 • 464 citations

Pichia pastoris as a host system for transformations.

1985 • 423 citations

Recombinant tumor necrosis factor and immune interferon act singly and in combination to reorganize human vascular endothelial cell monolayers.

1986 • 401 citations

Expression of thelacZgene from two methanol-regulated promoters inPichia pastoris

1987 • 364 citations

Tumor necrosis factor stimulates interleukin-1 and prostaglandin E2 production in resting macrophages

1986 • 344 citations

High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris

1987 • 332 citations

Growth of Hansenula polymorpha in a methanol-limited chemostat

1976 • 307 citations

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

1985 • 271 citations

High-Level Secretion of Glycosylated Invertase in the Methylotrophic Yeast, Pichia Pastoris

1987 • 247 citations

Molecular cloning and expression of human tumor necrosis factor and comparison with mouse tumor necrosis factor

1985 • 240 citations

Purification of Calf Prochymosin (Prorennin) Synthesized in Escherichia Coli

1984 • 227 citations

Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone

1985 • 201 citations

Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase.

1980 • 190 citations

Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase

1983 • 158 citations

Microbial Assimilation of Methanol

1973 • 138 citations

Recombinant tumor necrosis factor depresses cytochrome P450-dependent microsomal drug metabolism in mice

1986 • 112 citations

High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda

1984 • 103 citations

Biosynthesis and regulation of the peroxisomal methanol oxidase from the methylotrophic yeast Hansenula polymorpha

1984 • 100 citations

Unusually high-level expression of a foreign gene (hepatitis B virus core antigen) in Saccharomyces cerevisiae

1986 • 88 citations

Biological research on industrial yeasts

1987 • 84 citations

Biosynthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha

1983 • 72 citations

Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation

1983 • 70 citations

Efficient production of human tumour necrosis factor in Escherichia coli

1985 • 46 citations

Development of yeast transformation systems and construction of methanol-utilization-defective mutants of Pichia pastori by gene disruption

1987 • 43 citations

Invertase gene (SUC2) of Saccharomyces cerevisiae as a dominant marker for transformation of Pichia pastoris

1987 • 36 citations

Sendai virus induces high levels of tumor necrosis factor mRNA in human peripheral blood leukocytes

1986 • 36 citations

Expression of synthetic human lysozyme gene in Escherichia coli.

1985 • 6 citations