Reduced heat resistance of mutant spores after cloning and mutagenesis of the Bacillus subtilis gene encoding penicillin-binding protein 5

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

Rapid similarity searches of nucleic acid and protein data banks.

1983 • 1,577 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

Similar Amino Acid Sequences: Chance or Common Ancestry?

1981 • 855 citations

An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences

1982 • 801 citations

Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing

1982 • 744 citations

PENICILLIN-BINDING PROTEINS AND THE MECHANISM OF ACTION OF BETA-LACTAM ANTIBIOTICS

1983 • 698 citations

Computer methods to locate signals in nucleic acid sequences

1984 • 673 citations

The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)

1984 • 563 citations

[118] Enzymes that degrade bacterial cell walls

1966 • 518 citations

The Stability of Messenger Ribonucleic Acid during Sporulation in Bacillus subtilis

1971 • 441 citations

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

1981 • 423 citations

Graphic methods to determine the function of nucleic acid sequences

1984 • 367 citations

Gene Cloning in Streptomyces

1982 • 335 citations

Construction and properties of an integrable plasmid for Bacillus subtilis

1983 • 266 citations

A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions

1984 • 251 citations

Penicillin-binding Proteins and the Future of -Lactam Antibiotics: The Seventh Fleming Lecture

1983 • 203 citations

Glycopeptide transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions.

1966 • 186 citations

Structure of the peptidoglycan from spores of Bacillus subtilis

1972 • 145 citations

A new selective phage cloning vector, λ2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI

1984 • 144 citations

Insertional mutagenesis in Bacillus subtilis: mechanism and use in gene cloning

1982 • 139 citations

Use of Integrational Plasmid Vectors to Demonstrate the Polycistronic Nature of a Transcriptional Unit (spoIIA) Required for Sporulation of Bacillus subtilis

1984 • 131 citations

Mapping a cloned gene under sporulation control by inserttion of a drug resistance marker into the Bacillus subtilis chromosome

1980 • 130 citations

A computer program to enter DNA gel reading data into a computer

1984 • 123 citations

Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity.

1977 • 122 citations

Spherical E. coli due to elevated levels of D-alanine carboxypeptidase

1982 • 101 citations

Sequence of active site peptides from the penicillin-sensitive D-alanine carboxypeptidase of Bacillus subtilis. Mechanism of penicillin action and sequence homology to beta-lactamases.

1980 • 89 citations

Gene Amplification in Bacillus subtilis

1984 • 84 citations

Altered penicillin-binding components in penicillin-resistant mutants of Bacillus subtilis.

1976 • 83 citations

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls

1970 • 82 citations

Inactivation of D-Alanine Carboxypeptidase by Penicillins and Cephalosporins Is Not Lethal in Bacillus subtilis

1971 • 72 citations

d-Alanine Carboxypeptidase from Bacillus subtilis Membranes

1973 • 64 citations

Changes in penicillin-binding proteins during sporulation of Bacillus subtilis

1983 • 62 citations

Nucleotide sequence of the Bacillus subtilis trpE and trpD genes

1984 • 55 citations

Early sporulation gene spo0F: nucleotide sequence and analysis of gene product.

1983 • 50 citations

Relationship between cortex content and properties of Bacillus sphaericus spores

1976 • 47 citations

Identification of the binding protein which may be the target of penicillin action in Bacillus megaterium

1978 • 46 citations

Deletion of the penicillin-binding protein 5 gene of Escherichia coli

1980 • 46 citations

Primary structure of the COOH-terminal membranous segment of a penicillin-sensitive enzyme purified from two Bacilli.

1981 • 36 citations

Differential expression of penicillin-binding protein structural genes duringBacillus subtilissporulation

1983 • 34 citations

The role of cortex hydrolysis in the triggering of germination of Bacillus megaterium KM endospores

1982 • 30 citations

Synthesis of peptidoglycan by high molecular weight penicillin-binding proteins of Bacillus subtilis and Bacillus stearothermophilus.

1984 • 28 citations

The sporulation-specific penicillin-binding protein 5a from Bacillus subtilis is a <scp>dd</scp>-carboxypeptidase in vitro

1985 • 27 citations

Cloning thegyrAgene ofBacillus subtilis

1984 • 24 citations

SEQUENCE OF PENICILLIN-BINDING PROTEIN 5 OF ESCHERICHIA COLI

1983 • 24 citations

Alteration in the penicillin-binding profile of Bacillus megaterium during sporulation

1982 • 21 citations

Polarity and topology of DNA segregation and septation in cells and sporangia of the bacilli

1978 • 20 citations

グリコペブチドトランスペブチダーゼとD一アラニンカルボキシべプチダーゼ:べニシリン-感受性の酵素反応

1966 • 17 citations

<scp>d</scp> -Alanine Carboxypeptidase and Cell Wall Cross-Linking in Bacillus subtilis

1974 • 17 citations

The interaction of nocardicin A with the penicillin‐binding proteins of Bacillus megaterium KM

1983 • 3 citations