De novo design of the hydrophobic core of ubiquitin

Adaptation in natural and artificial systems

1975 • 36,178 citations

Adaptation in Natural and Artificial Systems

1992 • 14,282 citations

A Second Generation Force Field for the Simulation of Proteins, Nucleic Acids, and Organic Molecules

1995 • 12,826 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Calculation of protein extinction coefficients from amino acid sequence data

1989 • 5,198 citations

The OPLS [optimized potentials for liquid simulations] potential functions for proteins, energy minimizations for crystals of cyclic peptides and crambin

1988 • 4,676 citations

A new force field for molecular mechanical simulation of nucleic acids and proteins

1984 • 4,553 citations

Dominant forces in protein folding

1990 • 3,682 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

AREAS, VOLUMES, PACKING, AND PROTEIN STRUCTURE

1977 • 2,631 citations

1H, 13C and 15N chemical shift referencing in biomolecular NMR

1995 • 2,294 citations

Structure of ubiquitin refined at 1.8 Å resolution

1987 • 1,682 citations

Tertiary templates for proteins

1987 • 1,485 citations

A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper Mutants

1993 • 1,456 citations

The molten globule state as a clue for understanding the folding and cooperativity of globular‐protein structure

1989 • 1,446 citations

Study of the “molten globule” intermediate state in protein folding by a hydrophobic fluorescent probe

1991 • 1,231 citations

Response of a Protein Structure to Cavity-Creating Mutations and Its Relation to the Hydrophobic Effect

1992 • 933 citations

Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids

1993 • 737 citations

Characterization of a Helical Protein Designed from First Principles

1988 • 533 citations

Analysis of the relationship between side-chain conformation and secondary structure in globular proteins

1987 • 422 citations

De Novo Design, Expression, and Characterization of Felix: a Four-Helix Bundle Protein of Native-Like Sequence

1990 • 392 citations

Solvent denaturation

1978 • 383 citations

Contributions of the large hydrophobic amino acids to the stability of staphylococcal nuclease

1990 • 372 citations

Evidence for a three-state model of protein folding from kinetic analysis of ubiquitin variants with altered core residues

1996 • 347 citations

A New Approach to the Rapid Determination of Protein Side Chain Conformations

1991 • 345 citations

Database algorithm for generating protein backbone and side-chain co-ordinates from a Cα trace

1991 • 324 citations

De novo design of the hydrophobic cores of proteins

1995 • 301 citations

Protein design automation

1996 • 291 citations

Metal Ion-Dependent Modulation of the Dynamics of a Designed Protein

1993 • 252 citations

Folding and stability of a tryptophan-containing mutant of ubiquitin

1993 • 232 citations

Prediction of protein side-chain conformation by packing optimization

1991 • 228 citations

Early hydrogen-bonding events in the folding reaction of ubiquitin.

1992 • 220 citations

An analysis of packing in the protein folding problem

1993 • 219 citations

The role of internal packing interactions in determining the structure and stability of a protein

1991 • 208 citations

Similar Hydrophobic Replacements of Leu99 and Phe153 within the Core of T4 Lysozyme Have Different Structural and Thermodynamic Consequences

1993 • 200 citations

Thermodynamics of ubiquitin unfolding

1994 • 193 citations

What makes a protein a protein? Hydrophobic core designs that specify stability and structural properties

1996 • 192 citations

Accurate prediction of the stability and activity effects of site-directed mutagenesis on a protein core

1991 • 180 citations

Theoretical study of the contribution of aromatic side chains to the circular dichroism of basic bovine pancreatic trypsin inhibitor

1989 • 168 citations

High-level Misincorporation of Lysine for Arginine at AGA Codons in a Fusion Protein Expressed inEscherichia coli

1996 • 166 citations

Fast internal main-chain dynamics of human ubiquitin

1992 • 157 citations

Crystal packing, hydrogen bonding, and the effect of crystal forces on molecular conformation

1980 • 146 citations

De novo protein design: from molten globules to native-like states

1993 • 145 citations

Betadoublet: de novo design, synthesis, and characterization of a beta-sandwich protein.

1994 • 144 citations

Two-dimensional proton NMR study of human ubiquitin: a main chain directed assignment and structure analysis

1987 • 142 citations

A test of the "jigsaw puzzle" model for protein folding by multiple methionine substitutions within the core of T4 lysozyme.

1996 • 140 citations

Design and structural analysis of alternative hydrophobic core packing arrangements in bacteriophage T4 lysozyme

1992 • 130 citations

Optimal sequence selection in proteins of known structure by simulated evolution.

1994 • 130 citations

Internal Dynamics of Human Ubiquitin Revealed by ¹³C-Relaxation Studies of Randomly Fractionally Labeled Protein

1996 • 130 citations

Redesigning the hydrophobic core of a four‐helix‐bundle protein

1994 • 125 citations

The Role of Backbone Flexibility in the Accommodation of Variants That Repack the Core of T4 Lysozyme

1993 • 122 citations

Internal dynamics of human ubiquitin revealed by 13C-relaxation studies of randomly fractionally labeled protein

1996 • 122 citations

Sequential 1H NMR Assignments and Secondary Structure Identification of Human Ubiquitin

1987 • 114 citations

Active barnase variants with completely random hydrophobic cores.

1996 • 114 citations

The crystal structure of a mutant protein with altered but improved hydrophobic core packing.

1994 • 110 citations

Sequential proton NMR assignments and secondary structure identification of human ubiquitin

1987 • 97 citations

Engineering of betabellin 14D: Disulfide‐induced folding of a β‐sheet protein

1994 • 95 citations

Construction of side-chains in homology modelling

1989 • 93 citations

Binding of naphthalene dyes to the N and A conformers of bovine α-lactalbumin

1982 • 88 citations

Gene synthesis, expression, structures, and functional activities of site-specific mutants of ubiquitin.

1987 • 85 citations

A de novo designed protein shows a thermally induced transition from a native to a molten globule-like state

1992 • 79 citations

Equilibrium unfolding of Escherichia coli ribonuclease H: Characterization of a partially folded state

1994 • 71 citations

Functional and structural studies of a small F-actin binding domain

1992 • 42 citations

Energy minimization method using automata network for sequence and side‐chain conformation prediction from given backbone geometry

1994 • 41 citations

De novo design and creation of a stable artificial protein

1994 • 14 citations