A human pancreatic secretory trypsin inhibitor presenting a hypervariable highly constrained epitope via monovalent phagemid display

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

1985 • 3,886 citations

High efficiency transformation of E.coli by high voltage electroporation

1988 • 2,789 citations

Phage antibodies: filamentous phage displaying antibody variable domains

1990 • 2,346 citations

Searching for Peptide Ligands with an Epitope Library

1990 • 2,080 citations

By-passing immunization

1991 • 1,603 citations

Making antibody fragments using phage display libraries

1991 • 1,195 citations

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

1991 • 1,014 citations

Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli

1989 • 989 citations

Isolation of high affinity human antibodies directly from large synthetic repertoires.

1994 • 970 citations

Peptides on phage: a vast library of peptides for identifying ligands.

1990 • 928 citations

Random Peptide Libraries: a Source of Specific Protein Binding Molecules

1990 • 910 citations

Antibody-selectable filamentous fd phage vectors: affinity purification of target genes

1988 • 863 citations

[15] Libraries of peptides and proteins displayed on filamentous phage

1993 • 770 citations

Selection of phage antibodies by binding affinity

1992 • 514 citations

Human anti-self antibodies with high specificity from phage display libraries.

1993 • 480 citations

By-passing immunisation

1992 • 434 citations

Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.

1992 • 373 citations

Hormone phage: An enrichment method for variant proteins with altered binding properties

1990 • 360 citations

Selecting high-affinity binding proteins by monovalent phage display

1991 • 312 citations

A surface expression vector for antibody screening

1991 • 299 citations

Accelerated evolution in the reactive centre regions of serine protease inhibitors

1987 • 293 citations

Ovomucoid third domains from 100 avian species: isolation, sequences, and hypervariability of enzyme-inhibitor contact residues

1987 • 202 citations

Molecular evolution of proteins on filamentous phage. Mimicking the strategy of the immune system.

1992 • 201 citations

Directed evolution of a protein: selection of potent neutrophil elastase inhibitors displayed on M13 fusion phage.

1992 • 200 citations

M13 bacteriophage displaying disulfide-constrained microproteins

1993 • 176 citations

Human combinatorial antibody libraries to hepatitis B surface antigen.

1992 • 174 citations

An M13 phage library displaying random 38-amino-acid peptides as a source of novel sequences with affinity to selected targets

1993 • 157 citations

Combinatorial infection andin vivorecombination: a strategy for making large phage antibody repertoires

1993 • 153 citations

Design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage M13

1991 • 73 citations

Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type)

1991 • 56 citations

Selection of an anti-IGF-1 Fab from a Fab phage library created by mutagenesis of multiple CDR loops

1993 • 55 citations

Three-dimensional structure of a recombinant variant of human pancreatic secretory trypsin inhibitor (Kazal type)

1992 • 36 citations

Highly effective protease inhibitors from variants of human pancreatic secretory trypsin inhibitor (hPSTI): an assessment of 3-D structure-based protein design

1995 • 12 citations

A phasmid optimised for protein design projects: pMAMPF

1990 • 11 citations

Human leukocyte elastase inhibitors: designed variants of human pancreatic secretory trypsin inhibitor (hPSTI).

1990 • 9 citations

Solution Structure of a Variant of Human Pancreatic Secretory Trypsin Inhibitor Determined by Nuclear Magnetic Resonance Spectroscopy

1993 • 6 citations