By-passing immunisation

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Sequences of proteins of immunological interest

1984 • 3,427 citations

Phage antibodies: filamentous phage displaying antibody variable domains

1990 • 2,346 citations

By-passing immunization

1991 • 1,603 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein

1986 • 1,457 citations

Single-Chain Antigen-Binding Proteins

1988 • 1,450 citations

Canonical structures for the hypervariable regions of immunoglobulins

1987 • 1,357 citations

Replacing the complementarity-determining regions in a human antibody with those from a mouse

1986 • 1,356 citations

Making antibody fragments using phage display libraries

1991 • 1,195 citations

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.

1991 • 1,178 citations

Three-Dimensional Structure of an Antigen-Antibody Complex at 2.8 Å Resolution

1986 • 1,173 citations

Conformations of immunoglobulin hypervariable regions

1989 • 1,165 citations

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

1991 • 1,014 citations

Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli

1989 • 989 citations

Assembly of a Functional Immunoglobulin F _v Fragment in Escherichia coli

1988 • 973 citations

Man-made antibodies

1991 • 935 citations

Antibody-selectable filamentous fd phage vectors: affinity purification of target genes

1988 • 863 citations

Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda

1989 • 810 citations

The repertoire of human germline vH sequences reveals about fifty groups of VH segments with different hypervariable loops

1992 • 616 citations

Escherichia coli Secretion of an Active Chimeric Antibody Fragment

1988 • 575 citations

Selection of phage antibodies by binding affinity

1992 • 514 citations

Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.

1988 • 432 citations

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.

1991 • 415 citations

By–Passing Immunization: Building High Affinity Human Antibodies by Chain Shuffling

1992 • 405 citations

Structural repertoire of the human VH segments

1992 • 396 citations

Molecular events during maturation of the immune response to oxazolone

1985 • 380 citations

Direct clone characterization from plaques and colonies by the polymerase chain reaction

1989 • 327 citations

Organization of human immunoglobulin heavy chain diversity gene loci.

1988 • 317 citations

A surface expression vector for antibody screening

1991 • 299 citations

Kinetic maturation of an immune response

1991 • 274 citations

Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.

1991 • 239 citations

Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP.

1986 • 227 citations

Human IgA and IgG F(ab')2 that bind to staphylococcal protein A belong to the VHIII subgroup.

1991 • 217 citations

The smaller human VH gene families display remarkably little polymorphism.

1989 • 203 citations

Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.

1980 • 199 citations

Three-dimensional structure determination of an anti-2-phenyloxazolone antibody: the role of somatic mutation and heavy/light chain pairing in the maturation of an immune response.

1990 • 145 citations

An inhibition enzyme immunoassay for estimating relative antibody affinity and affinity heterogeneity

1988 • 134 citations

A 16/6 idiotype—positive anti‐DNA antibody is encoded by a conserved V_H gene with no somatic mutation

1988 • 132 citations

V_λ and J_λ‐C_λ gene segments of the human immunoglobulin λ light chain locus are separated by 14 kb and rearrange by a deletion mechanism

1991 • 116 citations

Physical map of the 3′ region of the human immunoglobulin heavy chain locus: clustering of autoantibody-related variable segments in one haplotype.

1991 • 115 citations

Modelling of the combining sites of three anti-lysozyme monoclonal antibodies and of the complex between one of the antibodies and its epitope.

1986 • 110 citations

Organization of variable region segments of the human immunoglobulin heavy chain: duplication of the D5 cluster within the locus and interchromosomal translocation of variable region segments.

1990 • 95 citations

Definition of the human immunoglobulin variable lambda (IGLV) gene subgroups

1990 • 84 citations

The V_ϰ gene repertoire in the human germ line

1990 • 70 citations

Cell selection strategies for making antibodies from variable gene libraries: trapping the memory pool

1992 • 54 citations

DETERMINATION OF ANTIBODY AFFINITY FOR HAPTENS AND ANTIGENS BY MEANS OF FLUORESCENCE QUENCHING.

1964 • 38 citations

The Croonian Lecture, 1989 Antibodies: a paradigm for the biology of molecular recognition

1990 • 29 citations

First genomic sequence of a human lg variable lambda gene belonging to subgroup III

1990 • 27 citations

Molecular modelling study of antigen binding to oxazolone-specific antibodies: the Ox1 idiotypic IgG and its mature variant with increased affinity to 2-phenyloxazolone

1990 • 17 citations