Synthesis of biotechnologically relevant heterologous proteins in filamentous fungi

Molecular chaperones in cellular protein folding

1996 • 3,540 citations

Common Principles of Protein Translocation Across Membranes

1996 • 1,081 citations

A nuclear DNA attachment element mediates elevated and position-independent gene activity

1989 • 597 citations

Getting the glycosylation right: Implications for the biotechnology industry

1996 • 455 citations

Transformation in fungi

1989 • 443 citations

More Gene Manipulations in Fungi

1991 • 321 citations

Genetics and Molecular Biology of Industrial Microorganisms

1989 • 319 citations

Control of messenger RNA stability in higher eukaryotes

1996 • 315 citations

Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase

1990 • 282 citations

Transformation in fungi.

1989 • 244 citations

Signal peptidases in prokaryotes and eukaryotes - a new protease family

1992 • 212 citations

Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene

1988 • 211 citations

Protein secretion in filamentous fungi — trying to understand a highly productive black box

1994 • 210 citations

A System for Production of Commercial Quantities of Human Lactoferrin: A Broad Spectrum Natural Antibiotic

1995 • 182 citations

Applied Molecular Genetics of Filamentous Fungi

1992 • 182 citations

Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion

1990 • 175 citations

Scaffold attachment regions increase reporter gene expression in stably transformed plant cells.

1993 • 175 citations

Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases

1992 • 169 citations

Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi

1989 • 167 citations

Commercial Levels of Chymosin Production by Aspergillus

1991 • 166 citations

Distinct cis-Acting Elements Mediate Clock, Light, and Developmental Regulation of the Neurospora crassa eas (ccg-2) Gene

1996 • 162 citations

Use of Recombinant DNA to Improve Production of Cephalosporin C By Cephalosporium acremonium

1989 • 148 citations

Nitrogen metabolite signalling involves the C-terminus and the GATA domain of the Aspergillus transcription factor AREA and the 3′ untranslated region of its mRNA.

1996 • 137 citations

Heterologous Gene Expression in Filamentous Fungi

1991 • 136 citations

The Intergenic Region between the Divergently Transcribed niiA and niaD Genes of Aspergillus nidulans Contains Multiple NirA Binding Sites Which Act Bidirectionally

1995 • 131 citations

Hen Egg White Lysozyme Expressed in and Secreted from, Aspergillus niger is Correctly Processed and Folded

1990 • 121 citations

Amplification of the isopenicillin N synthetase gene in a strain of Penicillium chrysogenum producing high levels of penicillin

1989 • 119 citations

Production of Cephalosporin Intermediates by Feeding Adipic Acid to Recombinant Penicillium chrysogenum Strains Expressing Ring Expansion Activity

1995 • 116 citations

The Production of Cephalosporin C by Acremonium chrysogenum is Improved by the Intracellular Expression of a Bacterial Hemoglobin

1993 • 115 citations

Efficient Production of Antibody Fragments by the Filamentous Fungus Trichoderma reesei

1993 • 114 citations

Regulation of Aspergillus nidulans penicillin biosynthesis and penicillin biosynthesis genes acvA and ipnA by glucose

1992 • 114 citations

Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed Aspergillus nidulans gpdA gene

1991 • 111 citations

Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae

1996 • 102 citations

Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae

1991 • 97 citations

Efficient integrative transformation of Cephalosporium acremonium

1987 • 92 citations

Efficient KEX2-Like Processing of a Glucoamylase-Interleukin-6 Fusion Protein by Aspergillus Nidulans and Secretion of Mature Interleukin-6

1991 • 90 citations

Use of reporter genes to identify recessive trans-acting mutations specifically involved in the regulation of Aspergillus nidulans penicillin biosynthesis genes

1995 • 88 citations

Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains

1996 • 87 citations

Heterologous Protein Production by Filamentous Fungi

1991 • 86 citations

Genetically Engineered Secretion of Active Human Interferon and a Bacterial Endoglucanase from Aspergillus Nidulans

1987 • 83 citations

Recombinant microbes for industrial and agricultural applications

1994 • 81 citations

Codon usage in Aspergillus nidulans

1991 • 80 citations

The effect of multiple copies of the upstream region on expression of the Aspergillus niger glucoamylase-encoding gene

1994 • 80 citations

Strategies for improving heterologous protein production from filamentous fungi

1994 • 78 citations

A reporter gene analysis of penicillin biosynthesis gene expression in Penicillium chrysogenum and its regulation by nitrogen and glucose catabolite repression

1994 • 77 citations

Identification of a major cis-acting DNA element controlling the bidirectionally transcribed penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) of Aspergillus nidulans

1996 • 73 citations

Heterologous gene expression in Aspergillus niger: a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme

1992 • 67 citations

Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants

1991 • 66 citations

A truncated glucoamylase gene fusion for heterologous protein secretion fromAspergillus niger

1993 • 64 citations

Heterologous gene expression in filamentous fungi

1989 • 60 citations

The Aspergillus nidulans Penicillin‐Biosynthesis Gene aat (penDE) is Controlled by a CCAAT‐Containing DNA Element

1996 • 59 citations

Construction of a 7-Aminocephalosporanic Acid (7ACA) Biosynthetic Operon and Direct Production of 7ACA in Acremonium Chrysogenum

1991 • 58 citations

Isolation and characterization of a 1,4-β-endoxylanase gene of A. awamori

1994 • 57 citations

Recombinant Microbes for Industrial and Agricultural Applications

2020 • 54 citations

Overexpression of two penicillin structural genes in Aspergillus nidulans

1995 • 53 citations

Multiple roles of the cellulase CBHI in enhancing production of fusion antibodies by the filamentous fungus Trichoderma reesei

1995 • 52 citations

Characterization of the Trichoderma reesei cbh2 promoter

1993 • 52 citations

Cloning of the Aspergillus niger gene encoding α-l-arabinofuranosidase A

1993 • 48 citations

Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae

1993 • 47 citations

Heterologous gene expression by filamentous fungi: secretion of human interleukin-6 by Aspergillus nidulans

1990 • 47 citations

An expression system based on the promoter region of the Aspergillus awamori 1,4-β-endoxylanase A gene

1996 • 43 citations

Mutations affecting extracellular protease production in the filamentous fungusAspergillus nidulans

1996 • 41 citations

Expression studies with the bidirectional pcbAB-pcbC promoter region from Acremonium chrysogenum using reporter gene fusions

1994 • 40 citations

Insertion intoAspergillus nidulans of functional UDP-GlcNAc: ?3-d-mannoside ?-1,2-N-acetylglucosaminyltransferase I, the enzyme catalysing the first committed step from oligomannose to hybrid and complex N-glycans

1995 • 40 citations

Protein targeting and secretion in filamentous fungi

1994 • 39 citations

A twin-reporter vector for simultaneous analysis of expression signals of divergently transcribed, contiguous genes in filamentous fungi

1991 • 37 citations

Molecular characterization and functional analysis in Aspergillus nidulans of the 5′-region of the Penicillium chrysogenum isopenicillin N synthetase gene

1991 • 37 citations

The effect of pre- and pro-sequences and multicopy integration on heterologous expression of the Fusarium solani pisi cutinase gene in Aspergillus awamori

1996 • 37 citations

Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins

1993 • 35 citations

Genetic engineering of ß-lactam antibiotic biosynthetic pathways in filamentous fungi

1992 • 32 citations

Development of a gene transfer system for Penicillium chrysogenum

1987 • 30 citations

Analysis of the regulation of the Aspergillus nidulans penicillin biosynthesis gene aat (penDE), which encodes acyl coenzyme A : 6-aminopenicillanic acid acyltransferase

1995 • 29 citations

A lacZ reporter fusion method for the genetic analysis of regulatory mutations in pathways of fungal secondary metabolism and its application to the Aspergillus nidulans penicillin pathway

1995 • 28 citations

High Level Expression ofFusariumAlkaline Protease Gene inAcremonium chrysogenum

1994 • 22 citations

Enzyme Production by Recombinant Aspergillus

2020 • 19 citations

Heterologous protein secretion directed by a repressible acid phosphatase system of Aspergillus niger

1993 • 19 citations

Characterization of an Aspartic Proteinase of Mucor pusillus expressed in Aspergillus oryzae

1993 • 16 citations

Secretion of active human lysozyme by acremonium chrysogenum using a fusarium alkaline protease promoter system

1995 • 15 citations

Genetic Manipulation of Fungi by DNA-Mediated Transformation

1995 • 13 citations

Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis resinae

1994 • 7 citations