Secretion of active human lysozyme by acremonium chrysogenum using a fusarium alkaline protease promoter system

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

Rapid preparation of DNA from filamentous fungi

1985 • 1,708 citations

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

High Level Expression of Recombinant Genes in Aspergillus Oryzae

1988 • 259 citations

Controlled Expression and Secretion of Bovine Chymosin in Aspergillus Nidulans

1987 • 154 citations

A Novel Fungal Expression System: Secretion of Active Calf Chymosin from the Filamentous Fungus Trichoderma Reesei

1989 • 135 citations

Hen Egg White Lysozyme Expressed in and Secreted from, Aspergillus niger is Correctly Processed and Folded

1990 • 121 citations

Proteases and the processing of precursors to secreted proteins in yeast

1988 • 97 citations

Heterologous Production of a Ligninolytic Enzyme: Expression of the Phlebia Radiata Laccase Gene in Trichoderma Reesei

1991 • 96 citations

Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus Nidulans

1987 • 91 citations

Efficient KEX2-Like Processing of a Glucoamylase-Interleukin-6 Fusion Protein by Aspergillus Nidulans and Secretion of Mature Interleukin-6

1991 • 90 citations

Genetically Engineered Secretion of Active Human Interferon and a Bacterial Endoglucanase from Aspergillus Nidulans

1987 • 83 citations

Human lysozyme: Sequencing of a cDNA, and expression and secretion by Saccharomyces cerevisiae

1988 • 67 citations

Differences between Saccharomyces cerevisiae and Bacillus subtilis in secretion of human lysozyme

1987 • 53 citations

Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

1989 • 36 citations

Expression of the Escherichia coli Enterotoxin Subunit B Gene in Aspergillus nidulans Directed by the AMDS Promoter

1989 • 26 citations

Expression of human lysozyme in an insoluble form in yeast

1987 • 23 citations

High Level Expression ofFusariumAlkaline Protease Gene inAcremonium chrysogenum

1994 • 22 citations

Cloning and Nucleotide Sequence of the Alkaline Protease Gene fromFusariumsp. S-19-5 and Expression inSacchavomyces cerevisiae

1994 • 20 citations

Expression of Synthetic Human Lysozyme Gene inEscherichia coli

1986 • 20 citations

Expression of synthetic human lysozyme gene in Escherichia coli.

1986 • 12 citations

Autoproteolytic Processing and Processing-dependent Secretion of Aspergillus oryzae Alkaline Protease in Yeast.

1991 • 5 citations