Heterologous Protein Production by Filamentous Fungi

Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

1986 • 4,785 citations

Codon usage and tRNA content in unicellular and multicellular organisms.

1985 • 1,724 citations

Transcription termination and 3′ processing: the end is in site!

1985 • 1,482 citations

High-velocity microprojectiles for delivering nucleic acids into living cells

1987 • 1,370 citations

Transformation of yeast by a replicating hybrid plasmid

1978 • 1,362 citations

EUKARYOTIC TRANSCRIPTIONAL REGULATORY PROTEINS

1989 • 1,299 citations

The signal peptide

1990 • 1,096 citations

Transformation of Aspergillus nidulans by using a trpC plasmid.

1984 • 773 citations

The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3′ termini

1985 • 652 citations

PREMEIOTIC INSTABILITY OF REPEATED SEQUENCES IN NEUROSPORA CRASSA

1990 • 648 citations

A nuclear DNA attachment element mediates elevated and position-independent gene activity

1989 • 597 citations

Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells.

1989 • 443 citations

Transformation in fungi

1989 • 443 citations

Production of Soluble Recombinant Proteins in Bacteria

1989 • 430 citations

Formation of Soluble Recombinant Proteins in Escherichia Coli is Favored by Lower Growth Temperature

1988 • 418 citations

Sequences important for gene expression in filamentous fungi

1986 • 405 citations

RNA Polymerase B (II) And General Transcription Factors

1990 • 355 citations

Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA

1979 • 314 citations

Development of a high-frequency transforming vector for Aspergillus nidulans

1985 • 309 citations

Heterologous Protein Secretion from Yeast

1985 • 299 citations

Isolation and characterization of an autonomously replicating sequence from Ustilago maydis.

1988 • 287 citations

Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase

1990 • 282 citations

Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

1985 • 279 citations

Regulation of gene expression by pH of the growth medium in Aspergillus nidulans

1986 • 265 citations

High Level Expression of Recombinant Genes in Aspergillus Oryzae

1988 • 259 citations

Escherichia coli ribosomes translate in vivo with variable rate.

1984 • 257 citations

Expression, Glycosylation, and Secretion of an Aspergillus Glucoamylase by Saccharomyces cerevisiae

1985 • 248 citations

High-Level Secretion of Glycosylated Invertase in the Methylotrophic Yeast, Pichia Pastoris

1987 • 247 citations

Modulation of Folding Pathways of Exported Proteins by the Leader Sequence

1988 • 243 citations

DNA-mediated transformation of the basidiomycete Coprinus cinereus.

1987 • 231 citations

Molecular mechanisms of transcriptional regulation in yeast

1990 • 224 citations

Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene

1988 • 211 citations

An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency

1991 • 198 citations

Direct and indirect gene replacements in Aspergillus nidulans.

1985 • 183 citations

Constitutive and inducible Saccharomyces cerevisiae promoters: evidence for two distinct molecular mechanisms.

1986 • 181 citations

Protein sorting by selective retention in the endoplasmic reticulum and Golgi stack

1987 • 179 citations

Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion

1990 • 175 citations

Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi

1989 • 167 citations

Recombinant dna in Filamentous Fungi: Progress and Prospects

1987 • 158 citations

Controlled Expression and Secretion of Bovine Chymosin in Aspergillus Nidulans

1987 • 154 citations

Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA.

1988 • 148 citations

A cosmid for selecting genes by complementation in Aspergillus nidulans : Selection of the developmentally regulated yA locus

1985 • 146 citations

Heterologous Gene Expression in Filamentous Fungi

1991 • 136 citations

A Novel Fungal Expression System: Secretion of Active Calf Chymosin from the Filamentous Fungus Trichoderma Reesei

1989 • 135 citations

Silicon carbide fiber-mediated DNA delivery into plant cells

1990 • 134 citations

Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae

1987 • 124 citations

Hen Egg White Lysozyme Expressed in and Secreted from, Aspergillus niger is Correctly Processed and Folded

1990 • 121 citations

In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids

1990 • 114 citations

The ethanol utilization regulon of Aspergillus nidulans: the alcA-alcR system as a tool for the expression of recombinant proteins

1991 • 113 citations

Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed Aspergillus nidulans gpdA gene

1991 • 111 citations

Yeast artificial chromosomes: tools for mapping and analysis of complex genomes

1990 • 110 citations

Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.

1987 • 109 citations

Non-Mendelian Inheritance of DNA-Induced Inositol Independence in Neurospora

1973 • 109 citations

Regulation of the glaA gene of Aspergillus niger

1990 • 105 citations

Low Molecular Weight High–Mannose Type Glycans in a Secreted Protein of the Filamentous Fungus Trichoderma Reesei

1987 • 97 citations

Proteases and the processing of precursors to secreted proteins in yeast

1988 • 97 citations

Construction of a shuttle vector for the filamentous fungus Neurospora crassa.

1983 • 97 citations

Industrial enzymology: a look towards the future

1989 • 97 citations

Genetic engineering of Trichoderma to produce strains with novel cellulase profiles

1991 • 96 citations

Enzyme production by recombinant Trichoderma reesei strains

1991 • 96 citations

Why use air-lift bioreactors?

1990 • 92 citations

Replication and expression of a bacterial--mitochondrial hybrid plasmid in the fungus Podospora anserina.

1982 • 91 citations

The secretory pathway in yeast

1982 • 91 citations

A full length cDNA clone for the alkaline protease from Aspergillus oryzae: Structural analysis and expression in Saccharomyces cerevisiae

1989 • 91 citations

A new, rapid and efficient transformation procedure for Neurospora

1984 • 89 citations

Isolation and structure of the pectin lyase D-encoding gene from Aspergillus niger

1990 • 89 citations

A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae

1989 • 89 citations

Molecular cloning, nucleotide sequence and expression of the gene encoding prepro‐polygalacturonasell of Aspergillus niger

1990 • 88 citations

Rhizomucor miehei triglyceride lipase is processed and secreted from transformedAspergillus oryzae

1989 • 85 citations

Genetically Engineered Secretion of Active Human Interferon and a Bacterial Endoglucanase from Aspergillus Nidulans

1987 • 83 citations

Transcription Elements and Factors of Rna Polymerase B Promoters of Higher Eukaryote

1988 • 82 citations

Transformation of yeast by agitation with glass beads.

1988 • 81 citations

The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5′ mutations

1987 • 78 citations

Control of Folding of Proteins Secreted by a High Expression Secretion Vector, pIN-III-ompA: 16-Fold Increase in Production of Active Subtilisin E in Escherichia Coli

1988 • 77 citations

Structure of the cutinase gene and detection of promoter activity in the 5'-flanking region by fungal transformation

1989 • 70 citations

Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate

1986 • 66 citations

Transformation of Aspergillus oryzae using the A. niger pyrG gene

1987 • 66 citations

Transcription and processing signals in the 3-phosphoglycerate kinase (PGK) gene from Aspergillus nidulans

1986 • 64 citations

Enrichment method for the isolation of auxotrophic mutants of Mucor using the polyene antibiotic N-glycosyl-polifungin

1984 • 61 citations

The transformation of mycelial spheroplasts of Neurospora crassa and the attempted isolation of an autonomous replicator

1984 • 59 citations

Construction of a 7-Aminocephalosporanic Acid (7ACA) Biosynthetic Operon and Direct Production of 7ACA in Acremonium Chrysogenum

1991 • 58 citations

Fungi: The Perfect Hosts?

1986 • 57 citations

Proteolytic events in the processing of secreted proteins in fungi

1991 • 57 citations

Transformation of filamentous fungi by electroporation

1990 • 56 citations

Carbon Source Control of Cellobiohydrolase I and II Formation by Trichoderma reesei

1991 • 53 citations

Cloning and expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum

1990 • 53 citations

The crystal structure of a mutant human lysozyme C77/95A with increased secretion efficiency in yeast

1991 • 51 citations

Upstream Elements Repress Premature Expression of an Aspergillus Developmental Regulatory Gene

1990 • 51 citations

Heterologous insertion of transforming DNA and generation of new deletions associated with transformation in Aspergillus nidulans

1986 • 46 citations

Activity and thermal stability of genetically truncated forms of Aspergillus glucoamylase

1990 • 44 citations

Transformation to senescence with plasmid like DNA in the ascomycete Podospora anserina

1980 • 43 citations

Isolation of Alkaline and Neutral Proteases from Aspergillus flavus var. columnaris , a Soy Sauce Koji Mold

1981 • 43 citations

Expression of heterologous proteins in Aspergillus

1991 • 43 citations

Transformation ofAspergillus awamori andA. niger by electroporation

1989 • 42 citations

Transformation of Penicillium chrysogenum with a dominant selectable marker

1988 • 42 citations

Production and Characteristics of Raw-Starch-Digesting α-Amylase from a Protease-Negative Aspergillus ficum Mutant

1986 • 39 citations

N-glycosylation and the production of recombinant glycoproteins

1989 • 38 citations

Yeast mutants with enhanced ability to secrete human lysozyme: Isolation and identification of a protease-deficient mutant

1989 • 37 citations

Fungi in the production of foods and food ingredients

1989 • 35 citations

Characterization of deletion derivatives of an autonomously replicatingNeurosporaplasmid

1984 • 34 citations

Plasmid recovery from transformants and the isolation of chromosomal DNA segments improving plasmid replication in Neurospora crassa

1985 • 33 citations

Germination of concentrated suspensions of spores from Aspergillus niger

1989 • 32 citations

Mutation and screening to increase chymosin yield in a genetically-engineered strain ofAspergillus awamori

1990 • 28 citations

Distant upstream regulatory sequences control the level of expression of the am (GDH) locus of Neurospora crassa

1990 • 27 citations

Non-homologous integration of transforming vectors in the fungus Podospora anserina: sequences of junctions at the integration sites

1988 • 27 citations

Expression and Secretion of Thaumatin fromAspergillus oryzae

1990 • 26 citations

Expression of the Escherichia coli Enterotoxin Subunit B Gene in Aspergillus nidulans Directed by the AMDS Promoter

1989 • 26 citations

Control of gene expression from the SUC2 promoter of Saccharomyces cerevisiae with the aid of a glucose analyser

1989 • 25 citations

Intergrative transformation of filamentous fungi with respect to biotechnological application

1986 • 25 citations

Heterologous protein secretion from Aspergillus niger in phosphate-buffered batch culture

1990 • 24 citations

Cloning and sequencing of the alkaline protease cDNA from Aspergillus oryzae.

1988 • 24 citations

Purification of an acid protease and a serine carboxypeptidase from Aspergillus niger using metal-chelate affinity chromatography

1985 • 23 citations

Effect of temperature on polygalacturonase production by Aspergillus niger

1990 • 22 citations

Scale up of cellulase production and utilization

1988 • 22 citations

Regulatory region of the Aspergillus nidulans argB gene

1988 • 21 citations

Increased Biomass Production in a Benchtop Fermentor

1989 • 19 citations

Developments in protoplast fusion in fungi.

1987 • 16 citations

Improvement of amyloglucosidase production of Aspergillus awamori by mutagenic treatments

1979 • 15 citations

Use of transformation to make targeted sequence alterations at theam (GDH) locus ofNeurospora

1989 • 14 citations

Introduction to Fungal Proteinases and Expression in Fungal Systems

1991 • 14 citations

Further Evidence on the Interspecific Protoplast Fusion betweenAspergillus oryzaeandAspergillus sojaeand Subsequent Haploidization, with Special Reference to Their Production of Some Hydrolyzing Enzymes

1990 • 12 citations

Production of semi-alkaline protease by Aspergillus niger

1988 • 9 citations

Analysis of Aspergillus niger transformants for single site integration and vector recombination

1989 • 9 citations

Extracellular enzyme production by haploids, heterocaryons and diploids of Aspergillus nidulans

1987 • 8 citations

The effect of proteases on the synthesis of glucoamylase by mutants of Aspergillus niger C.

1985 • 8 citations

Interspecific hybridization of Aspergillus awamori var. kawachi and Aspergillus oryzae by protoplast fusion.

1988 • 7 citations

Properties of Aspergillus nidulans neutral protease

1983 • 6 citations

Isolation and purification of a new protease from Aspergillus niger LCF no 9

1988 • 6 citations

Distribution of molecular weight and chemical form of manganese compounds in soybeans.

1988 • 3 citations