Overexpression in Escherichia coli, purification and characterization of the molecular chaperone HSC70

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

1970 • 5,738 citations

THE HEAT-SHOCK PROTEINS

1988 • 5,214 citations

Protein folding in the cell

1992 • 4,138 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases

1966 • 2,239 citations

A theory of gel filtration and its exeperimental verification

1964 • 1,549 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

Interaction of Hsp 70 with Newly Synthesized Proteins: Implications for Protein Folding and Assembly

1990 • 1,293 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease

1992 • 1,201 citations

MOLECULAR CHAPERONES

1991 • 1,089 citations

Heat shock, stress proteins, chaperones, and proteotoxicity

1991 • 1,069 citations

Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein

1990 • 1,035 citations

Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding

1992 • 965 citations

A Role for a 70-Kilodalton Heat Shock Protein in Lysosomal Degradation of Intracellular Proteins

1989 • 932 citations

Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.

1986 • 896 citations

Polypeptide chain binding proteins: Catalysts of protein folding and related processes in cells

1989 • 884 citations

Peptide Binding and Release by Proteins Implicated as Catalysts of Protein Assembly

1989 • 763 citations

Uncoating ATPase is a member of the 70 kilodalton family of stress proteins

1986 • 607 citations

Protein Sorting to Mitochondria: Evolutionary Conservations of Folding and Assembly

1990 • 494 citations

An enzyme that removes clathrin coats: purification of an uncoating ATPase.

1984 • 449 citations

Protein Aggregation in vitro and in vivo: A Quantitative Model of the Kinetic Competition between Folding and Aggregation

1991 • 440 citations

S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP

1989 • 421 citations

The E. coli dnaK gene product, the hsp70 homolog, can reactivate heat-inactivated RNA polymerase in an ATP hydrolysis-dependent manner

1990 • 407 citations

On the Role of Heme in the Formation of the Structure of Cytochrome c

1973 • 388 citations

Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides.

1985 • 365 citations

Complex Interactions Among Members of an Essential Subfamily of hsp70 Genes in Saccharomyces Cerevisiae

1987 • 358 citations

Interaction of hsp70 with unfolded proteins: effects of temperature and nucleotides on the kinetics of binding.

1991 • 332 citations

Protein Folding in the Cell: The Role of Molecular Chaperones Hsp70 and Hsp60

1992 • 306 citations

Heat shock proteins: molecular chaperones of protein biogenesis.

1993 • 272 citations

Kinetic analysis of guanosine 5'-triphosphate hydrolysis associated with tubulin polymerization

1981 • 270 citations

Regulation of Hsp70 function by a eukaryotic DnaJ homolog.

1992 • 262 citations

The ATPase core of a clathrin uncoating protein.

1987 • 259 citations

Structure and Morphology of Protein Inclusion Bodies in Escherichia Coli

1991 • 238 citations

Enzymatic recycling of clathrin from coated vesicles

1986 • 237 citations

Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.

1987 • 225 citations

SSC1, a member of the 70-kDa heat shock protein multigene family of Saccharomyces cerevisiae, is essential for growth.

1987 • 205 citations

Role of the Escherichia coli DnaK and DnaJ heat shock proteins in the initiation of bacteriophage lambda DNA replication.

1988 • 194 citations

A hypothetical model for the peptide binding domain of hsp70 based on the peptide binding domain of HLA.

1991 • 191 citations

Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins.

1992 • 176 citations

Constitutively Expressed Rat mRNA Encoding a 70-Kilodalton Heat-Shock-Like Protein

1985 • 164 citations

Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis

1990 • 159 citations

Dissociation of clathrin coats coupled to the hydrolysis of ATP: role of an uncoating ATPase.

1984 • 153 citations

Superproduction and rapid purification of Escherichia coli aspartate transcarbamylase and its catalytic subunit under extreme derepression of the pyrimidine pathway.

1985 • 135 citations

Which came first, MHC class I or class II?

1991 • 132 citations

Unfolded proteins stimulate molecular chaperone Hsc70 ATPase by accelerating ADP/ATP exchange

1992 • 132 citations

A sensitive and precise isotopic assay of ATPase activity

1978 • 127 citations

Specialized Nucleoprotein Structures at the Origin of Replication of Bacteriophage λ

1989 • 125 citations

DnaK, hsp73, and their molten globules. Two different ways heat shock proteins respond to heat.

1992 • 118 citations

Use of gel chromatography for determination of size and molecular weight of proteins: Further caution

1980 • 109 citations

Dissociation of clathrin from coated vesicles by the uncoating ATPase.

1990 • 109 citations

A role for clathrin light chains in the recognition of clathrin cages by ‘uncoating ATPase’

1984 • 105 citations

Purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins

1986 • 104 citations

Constitutively expressed rat mRNA encoding a 70-kilodalton heat-shock-like protein.

1985 • 104 citations

A possible model for the concerted allosteric transition in Escherichia coli aspartate transcarbamylase as deduced from site-directed mutagenesis studies

1988 • 81 citations

Role of translation and attenuation in the control of pyrBI operon expression in Escherichia coli K-12

1985 • 79 citations

Coated vesicles

1980 • 75 citations

ATP catalyzes the sequestration of clathrin during enzymatic uncoating.

1985 • 73 citations

Trimeric binding of the 70-kD uncoating ATPase to the vertices of clathrin triskelia: a candidate intermediate in the vesicle uncoating reaction.

1989 • 59 citations

Constitutive HSP70: Oligomerization and its dependence on ATP binding

1992 • 42 citations

Aspartyl residue 10 is essential for ATPase activity of rat hsc70.

1993 • 40 citations

Propagation of allosteric changes through the catalytic-regulatory interface of Escherichia coli aspartate transcarbamylase

1988 • 23 citations

Inhibitory effects of HSP70 chaperones on nascent polypeptides

1992 • 19 citations

Biochemical and Biophysical Comparison of Bacterial DnaK and Mammalian hsc73, Two Members of an Ancient Stress Protein Family

1990 • 8 citations