Protein targeting to and translocation across the membrane of the endoplasmic reticulum

The GTPase superfamily: a conserved switch for diverse cell functions

1990 • 2,330 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

A protein-conducting channel in the endoplasmic reticulum

1991 • 613 citations

Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle

1989 • 474 citations

Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast.

1990 • 435 citations

A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum.

1987 • 418 citations

Sec61p and BiP directly facilitate polypeptide translocation into the ER

1992 • 370 citations

Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex

1991 • 359 citations

Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP–binding domains

1989 • 357 citations

Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum.

1992 • 352 citations

Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast.

1989 • 339 citations

Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle.

1986 • 337 citations

The signal sequence of nascent preprolactin interacts with the 54K polypeptide of the signal recognition particle

1986 • 336 citations

In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent post-translational translocation of the prepro-α-factor

1986 • 332 citations

A protein of the endoplasmic reticulum involved early in polypeptide translocation

1992 • 322 citations

The signal recognition particle in S. cerevisiae

1991 • 299 citations

The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide

1989 • 293 citations

Oligosaccharyltransferase activity is associated with a protein complex composed of ribophorins I and II and a 48 kd protein

1992 • 291 citations

A signal sequence receptor in the endoplasmic reticulum membrane

1987 • 291 citations

Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes

1978 • 281 citations

Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants

1985 • 277 citations

The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence.

1990 • 256 citations

Each of the activities of signal recognition particle (SRP) is contained within a distinct domain: Analysis of biochemical mutants of SRP

1988 • 248 citations

Requirement of GTP Hydrolysis for Dissociation of the Signal Recognition Particle from Its Receptor

1991 • 222 citations

Secretion in yeast: translocation and glycosylation of prepro-α-factor in vitro can occur via an ATP-dependent post-translational mechanism

1986 • 185 citations

Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain

1988 • 178 citations

The 54-kD protein of signal recognition particle contains a methionine-rich RNA binding domain.

1990 • 170 citations

Protein translocation across the endoplasmic reticulum membrane: identification by photocross-linking of a 39-kD integral membrane glycoprotein as part of a putative translocation tunnel.

1989 • 167 citations

Myoblast diversity in skeletal myogenesis: How much and to what end?

1992 • 164 citations

The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences.

1992 • 149 citations

Topology of signal recognition particle receptor in endoplasmic reticulum membrane

1985 • 140 citations

Yeast Sec proteins interact with polypeptides traversing the endoplasmic reticulum membrane

1992 • 138 citations

Identification of a ribosome receptor in the rough endoplasmic reticulum

1990 • 134 citations

Characterization of secretory protein translocation: ribosome-membrane interaction in endoplasmic reticulum.

1986 • 121 citations

Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomes

1990 • 118 citations

Pause transfer: A topogenic sequence in apolipoprotein B mediates stopping and restarting of translocation

1992 • 116 citations

Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth.

1989 • 115 citations

Binding sites of the 19-kDa and 68/72-kDa signal recognition particle (SRP) proteins on SRP RNA as determined in protein-RNA "footprinting".

1988 • 112 citations

Signal recognition particle receptor is important for cell growth and protein secretion in Saccharomyces cerevisiae.

1992 • 109 citations

A nascent membrane protein is located adjacent to ER membrane proteins throughout its integration and translation.

1991 • 109 citations

Binding sites of the 9- and 14-kilodalton heterodimeric protein subunit of the signal recognition particle (SRP) are contained exclusively in the Alu domain of SRP RNA and contain a sequence motif that is conserved in evolution.

1991 • 105 citations

The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19

1992 • 105 citations

A large presecretory protein translocates both cotranslationally, using signal recognition particle and ribosome, and post-translationally, without these ribonucleoparticles, when synthesized in the presence of mammalian microsomes.

1990 • 104 citations

The identification of proteins in the proximity of signal-anchor sequences during their targeting to and insertion into the membrane of the ER.

1991 • 104 citations

The signal sequence receptor has a second subunit and is part of a translocation complex in the endoplasmic reticulum as probed by bifunctional reagents.

1990 • 103 citations

Binding protein BiP is required for translocation of secretory proteins into the endoplasmic reticulum in Saccharomyces cerevisiae.

1991 • 102 citations

Mutants in three novel complementation groups inhibit membrane protein insertion into and soluble protein translocation across the endoplasmic reticulum membrane of Saccharomyces cerevisiae.

1992 • 102 citations

The signal sequence interacts with the methionine-rich domain of the 54-kD protein of signal recognition particle.

1991 • 101 citations

Antiribophorin antibodies inhibit the targeting to the ER membrane of ribosomes containing nascent secretory polypeptides.

1990 • 83 citations

Protein translocation across the ER requires a functional GTP binding site in the alpha subunit of the signal recognition particle receptor.

1992 • 82 citations

The signal sequence receptor, unlike the signal recognition particle receptor, is not essential for protein translocation

1992 • 78 citations

Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli.

1989 • 74 citations

SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity

1992 • 74 citations

ER translocation intermediates are adjacent to a nonglycosylated 34-kD integral membrane protein.

1991 • 67 citations

A membrane component of the endoplasmic reticulum that may be essential for protein translocation.

1989 • 61 citations

Interaction of protein SRP19 with signal recognition particle RNA lacking individual RNA-helices

1991 • 52 citations

Biochemical fractionation and assembly of the membrane components that mediate nascent chain targeting and translocation

1991 • 50 citations

Membrane Biogenesis and Protein Targeting

1992 • 48 citations

Isolation of a Yeast Gene, SRH1, That Encodes a Homblogue of the 54K Subunit of Mammalian Signal Recognition Particle

1990 • 46 citations

Ribosome binding to the endoplasmic reticulum: a 180-kD protein identified by crosslinking to membrane-bound ribosomes is not required for ribosome binding activity.

1991 • 42 citations

Identification of a Membrane Protein Responsible for Ribosome Binding in Rough Microsomal Membranes

1991 • 41 citations

A mutant of 7SL RNA in Yarrowia lipolytica affecting the synthesis of a secreted protein.

1992 • 39 citations

Reconstitution of translocation-competent membrane vesicles from detergent-solubilized dog pancreas rough microsomes.

1989 • 38 citations

Fluorescence-detected assembly of the signal recognition particle: binding of the two SRP protein heterodimers to SRP RNA is noncooperative

1992 • 37 citations

Characterization of the rough endoplasmic reticulum ribosome-binding activity

1991 • 36 citations

A mutation in the signal recognition particle 7S RNA of the yeast Yarrowia lipolytica preferentially affects synthesis of the alkaline extracellular protease: in vivo evidence for translational arrest.

1992 • 31 citations

Reconstitution of protein translocation activity from partially solubilized microsomal vesicles.

1990 • 29 citations

SRH1 protein, the yeast homologue of the 54 kDa subunit of signal recognition particle, is involved in ER translocation of secretory proteins

1991 • 28 citations

Studies on membrane proteins involved in ribosome binding on the rough endoplasmic reticulum. Ribophorins have no ribosome-binding activity

1987 • 20 citations

An ATP-binding membrane protein is required for protein translocation across the endoplasmic reticulum membrane.

1991 • 18 citations

Photoaffinity labeling of dog pancreas microsomes with 8‐azido‐ATP inhibits association of nascent preprolactin with the signal sequence receptor complex

1991 • 9 citations

Interactions of signal peptides with signal-recognition particle

1990 • 2 citations