The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

1974 • 3,603 citations

An improved assay for nanomole amounts of inorganic phosphate

1979 • 2,157 citations

Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements

1983 • 1,316 citations

Information content of binding sites on nucleotide sequences

1986 • 953 citations

Translational Initiation in Prokaryotes

1981 • 785 citations

Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

1979 • 722 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325

1980 • 616 citations

Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9

1979 • 494 citations

[27] Glutathione S-transferases (rat and human)

1981 • 410 citations

A role for mRNA secondary structure in the control of translation initiation

1982 • 344 citations

Organization of the recA gene of Escherichia coli.

1980 • 258 citations

lon gene product of Escherichia coli is a heat-shock protein

1984 • 221 citations

Maximizing Gene Expression

1986 • 213 citations

The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments

1980 • 206 citations

Optimizing the expression inE. coliof a synthetic gene encoding somatomedin-C (IGF-I)

1985 • 144 citations

Nucleolytic processing of ribonucleic acid transcripts in procaryotes

1986 • 141 citations

Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.

1984 • 128 citations

Recognition of messenger RNA during translational initiation in Escherichia coli

1984 • 124 citations

Inefficient translation initiation causes premature transcription termination in the IacZ gene

1986 • 122 citations

lacZ translation initiation mutations

1984 • 112 citations

Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5‘ of an ATG initiation codon

1983 • 112 citations

Amplification of the aroA gene from Escherichia coli results in tolerance to the herbicide glyphosate

1983 • 106 citations

Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene

1982 • 97 citations

The influence of mRNA primary and secondary structure on human IFN-γ gene expression inE. coli

1984 • 97 citations

Structural analysis of a maize gene coding for glutathione-S-transferase involved in herbicide detoxification

1986 • 94 citations

Changes in the half-life of ribosomal protein messenger RNA caused by translational repression

1986 • 92 citations

Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expression

1985 • 90 citations

The biochemistry and molecular biology of atrial natriuretic factor

1985 • 83 citations

Portable Shine-Dalgarno Regions: A System for a Systematic Study of Defined Alterations of Nucleotide Sequences withinE. coliRibosome Binding Sites

1983 • 67 citations

Proteolytic processing of atriopeptin prohormone.

1986 • 67 citations

Maximizing gene expression

1987 • 63 citations

Secondary structure as primary determinant of the efficiency of ribosomal binding sites inEscherichia coli

1986 • 61 citations

Nucleolytic processing of ribonucleic acid transcripts in procaryotes.

1986 • 61 citations

Escherichia coli translational initiation factor IF3: a unique case of translational regulation.

1984 • 60 citations

Effects of alterations in the translation control region on bacterial gene expression: use of cat gene constructs transcribed from the lac promoter as a model system

1984 • 54 citations

Nocleotide sequence of tbetmrlocus ofAgrobacterium tumefacienspTi T37 T-DNA

1984 • 49 citations

Chloramphenicol Acetyltransferases Specified by fi ⁻ R Factors

1973 • 33 citations

Expression of human interferon genes using therecApromoter ofEscherichia coli

1983 • 29 citations

The effects of hybrid ribosome-binding-site variants on the expression of human Interferon-β in Escherichia coli

1985 • 25 citations

Use of a portable ribosome-binding site for maximizing expression of a eukaryotic gene in Escherichia coli

1986 • 12 citations