Use of a portable ribosome-binding site for maximizing expression of a eukaryotic gene in Escherichia coli

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Experiments in molecular genetics

1972 • 28,260 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

1974 • 3,603 citations

Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information

1981 • 3,596 citations

T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity

1978 • 3,314 citations

Structure and expression of a cloned cDNA for human interleukin-2

1983 • 1,176 citations

Plasmid screening at high colony density

1980 • 1,100 citations

The tac promoter: a functional hybrid derived from the trp and lac promoters.

1983 • 1,001 citations

Translational Initiation in Prokaryotes

1981 • 785 citations

A bacterial clone synthesizing proinsulin.

1978 • 515 citations

A role for mRNA secondary structure in the control of translation initiation

1982 • 344 citations

Molecular cloning of human interleukin 2 cDNA and its expression inE. coli

1983 • 307 citations

Purified λ regulatory protein cII positively activates promoters for lysogenic development

1981 • 241 citations

Construction and analysis of in vivo activity of E. coll promoter hybrids and promoter mutants that alter the −35 to −10 spacing

1982 • 219 citations

Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

1984 • 131 citations

Construction of versatile expression cloning vehicles using the lipoprotein gene of Escherichia coli.

1982 • 129 citations

Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.

1984 • 128 citations

Genetic Signals and Nucleotide Sequences in Messenger RNA

1979 • 114 citations

Increased Synthesis inE. coliof Fibroblast and Leukocyte Interferons Through Alterations in Ribosome Binding Sites

1982 • 106 citations

Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene

1982 • 97 citations

Differential translation efficiency explains discoordinate expression of the galactose operon

1981 • 72 citations

Portable Shine-Dalgarno Regions: A System for a Systematic Study of Defined Alterations of Nucleotide Sequences withinE. coliRibosome Binding Sites

1983 • 67 citations

The construction and characterisation of plasmid vectors suitable for the expression of all DNA phases under the control of the E. coli tryptophan promoter

1980 • 62 citations

Effect of the sequences upstream from the ribosome-binding site on the yield of protein from the cloned gene for phage MS2 coat protein

1983 • 60 citations

Mutations that affect lamB gene expression at a posttranscriptional level.

1981 • 53 citations

Construction and characterization of a novel two-plasmid system for accomplishing temperature-regulated, amplified expression of cloned adventitious genes in Escherichia coli

1981 • 49 citations

High expression of cloned genes in E. coli and its consequences

1983 • 45 citations

Gene expression: chemical synthesis ofE. coliribosome binding sites and their use in directing the expression of mammalian proteins in bacteria

1982 • 44 citations

Mutations upstream of the ribosome-binding site affect translational efficiency

1985 • 44 citations

Enzymic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates

1980 • 41 citations

Escherichia coli plasmid vectors containing synthetic translational initiation sequences and ribosome binding sites fused with the lacZ gene

1982 • 27 citations

In vitro synthesis of the tryptophan operon leader peptides of Escherichia coli, Serratia marcescens, and Salmonella typhimurium.

1983 • 25 citations

Expression in Bacillus subtilis of the gene for human urogastrone using synthetic ribosome binding sites

1984 • 14 citations