Differential Roles of de Novo Sphingolipid Biosynthesis and Turnover in the “Burst” of Free Sphingosine and Sphinganine, and Their 1-Phosphates and N-Acyl-Derivatives, That Occurs upon Changing the Medium of Cells in Culture

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

[10] Assay of inorganic phosphate, total phosphate and phosphatases

1966 • 3,302 citations

Programmed Cell Death Induced by Ceramide

1993 • 1,779 citations

The sphingomyelin cycle and the second messenger function of ceramide.

1994 • 1,255 citations

ADVANCES IN LIPID RESEARCH

1966 • 1,199 citations

Sphingosine inhibition of protein kinase C activity and of phorbol dibutyrate binding in vitro and in human platelets.

1986 • 1,150 citations

Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme

1991 • 1,094 citations

Sphingosine-1-phosphate as second messenger in cell proliferation induced by PDGF and FCS mitogens

1993 • 879 citations

Sphingosine-1-phosphate, a novel lipid, involved in cellular proliferation.

1991 • 627 citations

Methods for methanolysis of sphingolipids and direct determination of long‐chain bases by gas chromatography

1965 • 533 citations

Biochemistry of Lipids, Lipoproteins and Membranes

1992 • 438 citations

Intracellular Calcium Release Mediated by Sphingosine Derivatives Generated in Cells

1990 • 391 citations

Subcellular localization and membrane topology of serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase in mouse liver.

1992 • 343 citations

Quantitation of free sphingosine in liver by high-performance liquid chromatography

1988 • 315 citations

Inhibition of the oxidative burst in human neutrophils by sphingoid long-chain bases. Role of protein kinase C in activation of the burst.

1986 • 294 citations

Plasma membrane fractions from rat liver contain a phosphatidate phosphohydrolase distinct from that in the endoplasmic reticulum and cytosol.

1991 • 271 citations

Sphingosine 1-phosphate generated in the endoplasmic reticulum membrane activates release of stored calcium.

1994 • 253 citations

Cell regulation by sphingosine and more complex sphingolipids

1991 • 242 citations

A treatise on the chemical constitution of the brain.

1963 • 236 citations

Sphingosine stimulates cellular proliferation via a protein kinase C-independent pathway.

1990 • 230 citations

Inhibition of sphingosine kinase in vitro and in platelets. Implications for signal transduction pathways.

1992 • 193 citations

Enzymology of long-chain base synthesis by liver: Characterization of serine palmitoyltransferase in rat liver microsomes

1984 • 182 citations

Metabolism and intracellular transport of glycosphingolipids

1990 • 178 citations

Specificity in Ceramide Biosynthesis from Long Chain Bases and Various Fatty Acyl Coenzyme A's by Brain Microsomes

1970 • 176 citations

Inhibition of Na,K-ATPase and sodium pump by protein kinase C regulators sphingosine, lysophosphatidylcholine, and oleic acid.

1990 • 159 citations

Sphingosine-1-phosphate, a metabolite of sphingosine, increases phosphatidic acid levels by phospholipase D activation

1992 • 154 citations

Characterization of serine palmitoyltransferase activity in Chinese hamster ovary cells

1983 • 151 citations

Disruption of sphingolipid metabolism and stimulation of DNA synthesis by fumonisin B1. A molecular mechanism for carcinogenesis associated with Fusarium moniliforme.

1994 • 147 citations

Biosynthesis of long-chain (sphingoid) bases from serine by LM cells. Evidence for introduction of the 4-trans-double bond after de novo biosynthesis of N-acylsphinganine(s).

1986 • 145 citations

Biosynthesis of sphingolipids: Dihydroceramide and not sphinganine is desaturated by cultured cells

1992 • 144 citations

Subcellular localization and membrane topology of sphingosine-1-phosphate lyase in rat liver

1991 • 142 citations

Activation of phospholipase D by sphingoid bases in NG108-15 neural-derived cells.

1990 • 134 citations

A stereoselective synthesis of sphingosine, a protein kinase c inhibitor.

1988 • 119 citations

Characterization of a major protein in GLUT4 vesicles. Concentration in the vesicles and insulin-stimulated translocation to the plasma membrane.

1994 • 118 citations

Synthesis of C18- and C20-Dihydrosphingosines, Ketodihydrosphingosines, and Ceramides by Microsomal Preparations from Mouse Brain

1970 • 114 citations

Free Sphingosine Formation from Endogenous Substrates by a Liver Plasma Membrane System with a Divalent Cation Dependence and a Neutral pH Optimum

1989 • 102 citations

Regulation of phosphatidate phosphatase activity from the yeast Saccharomyces cerevisiae by sphingoid bases

1993 • 99 citations

Sphingosine inhibits phosphatidate phosphohydrolase in human neutrophils by a protein kinase C-independent mechanism

1991 • 97 citations

Kinetics of long-chain (sphingoid) base biosynthesis in intact LM cells: effects of varying the extracellular concentrations of serine and fatty acid precursors of this pathway

1988 • 97 citations

Modulation of the free sphingosine levels in human neutrophils by phorbol esters and other factors.

1988 • 97 citations

Modulation of sphingolipid biosynthesis in primary cultured neurons by long chain bases.

1990 • 96 citations

Characteristics of the growth inhibition and cytotoxicity of long-chain (sphingoid) bases for Chinese hamster ovary cells

1990 • 86 citations

Sphingosine 1-phosphate, a novel signaling molecule, stimulates DNA binding activity of AP-1 in quiescent Swiss 3T3 fibroblasts.

1994 • 86 citations

Retinoblastoma protein dephosphorylation induced by D-erythro-sphingosine.

1992 • 82 citations

Free sphingoid bases in normal murine tissues

1988 • 80 citations

Sphingosine activation of protein kinases in Jurkat T cells. In vitro phosphorylation of endogenous protein substrates and specificity of action.

1992 • 80 citations

Direct evidence by carbon‐13 NMR spectroscopy for the erythro configuration of the sphingoid moiety in Gaucher cerebroside and other natural sphingolipids

1985 • 78 citations

Inhibition of serine palmitoyltransferase in vitro and long-chain base biosynthesis in intact Chinese hamster ovary cells by .beta.-chloroalanine

1988 • 78 citations

Metabolism of Sphingosine Bases, XV. Enzymatic Degradation of 4t-Sphingenine 1-phosphate (Sphingosine 1-phosphate) to 2t-Hexadecen-1-al and Ethanolamine phosphate

1970 • 56 citations

A Long Chain Terpenyl Pyrophosphate Synthetase from Micrococcus lysodeikticus

1967 • 52 citations

In Vivo Studies on the Introduction of the 4-t-Double Bond of the Sphingenine Moiety of Rat Brain Ceramides

1973 • 52 citations

Enzymatic Formation of Dihydrosphingosine 1-Phosphate

1970 • 50 citations

Biosynthesis of sphingolipid bases

1971 • 44 citations

Polyunsaturated fatty acids in human nutrition

1993 • 41 citations

Regulation of phospholipase D by sphingosine involves both protein kinase C-dependent and -independent mechanisms in NIH 3T3 fibroblasts

1992 • 37 citations

The 5' proximal helix of 16S rRNA is involved in the binding of streptomycin to the ribosome.

1993 • 34 citations

Sphingolipid Biosynthesis by Rat Liver Cells: Effects of Serine, Fatty Acids and Lipoproteins

1989 • 32 citations

Bimodal distribution of phosphatidic acid phosphohydrolase in NG108–15 cells

1992 • 30 citations

Ethanol potentiates the stimulatory effects of phorbol ester, sphingosine and 4‐hydroxynonenal on the hydrolysis of phosphatidylethanolamine in NIH 3T3 cells

1991 • 26 citations

Stereoselective synthesis of (3R,4S)-statine utilising the iron acetyl complex [(η5-C5H5)Fe(CO)(PPh3)COMe] as a chiral acetate enolate equivalent.

1993 • 24 citations

Formation of endogenous free sphingoid bases in cells induced by changing medium conditions

1994 • 23 citations

Determination of phospholipase D‐mediated hydrolysis of phosphatidylethanolamine

1991 • 22 citations

Rapid turnover of sphingosine synthesized de novo from [14C]serine by Chinese hamster ovary cells

1988 • 13 citations

Ceramide Metabolism Compartmentalized in the Endoplasmic Reticulum and Golgi Apparatus

1994 • 13 citations

Sphingosine and Other Long-Chain Bases That Alter Cell Behavior

1994 • 11 citations