Biochemical and Functional Characterization of a Recombinant GTPase, Rab5, and Two of Its Mutants

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Enzyme structure and mechanism

1977 • 2,935 citations

Enzyme structure and mechanism

1978 • 2,897 citations

All ras proteins are polyisoprenylated but only some are palmitoylated

1989 • 1,806 citations

The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway

1992 • 1,342 citations

rab5 controls early endosome fusion in vitro

1991 • 1,090 citations

Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis.

1994 • 948 citations

The guanine nucleotide activating site of the regulatory component of adenylate cyclase. Identification by ligand binding.

1982 • 542 citations

Biological and biochemical properties of human rasH genes mutated at codon 61

1986 • 538 citations

GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex.

1992 • 497 citations

Hypervariable C-termmal domain of rab proteins acts as a targeting signal

1991 • 394 citations

The Human Rab Genes Encode a Family of GTP-binding Proteins Related to Yeast YPT1 and SEC4 Products Involved in Secretion

1989 • 387 citations

Friends and family: The role of the rab GTPases in vesicular traffic

1993 • 364 citations

Differential regulation of rasGAP and neurofibromatosis gene product activities

1991 • 338 citations

Rab geranylgeranyl transferase. A multisubunit enzyme that prenylates GTP-binding proteins terminating in Cys-X-Cys or Cys-Cys.

1992 • 323 citations

cDNA cloning of component A of Rab geranylgeranyl transferase and demonstration of its role as a Rab escort protein

1993 • 322 citations

Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic.

1989 • 314 citations

The structure of Ras protein: a model for a universal molecular switch

1991 • 310 citations

Microtubule- and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells

1990 • 299 citations

Purification of component A of Rab geranylgeranyl transferase: Possible identity with the choroideremia gene product

1992 • 296 citations

Expression of p21 proteins in Escherichia coli and stereochemistry of the nucleotide-binding site.

1986 • 287 citations

The ras-related YPT1 gene product in yeast: A GTP-binding protein that might be involved in microtubule organization

1986 • 281 citations

Fluoride is not an activator of the smaller (20-25 kDa) GTP-binding proteins

1991 • 267 citations

The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport.

1992 • 259 citations

Synthesis in Escherichia coli of GTPase-deficient mutants of Gsα

1989 • 247 citations

Evidence for the involvement of Rab3A in Ca(2+)-dependent exocytosis from adrenal chromaffin cells.

1994 • 224 citations

Mutations in the GTP-binding site of GSα alter stimulation of adenylyl cyclase

1989 • 209 citations

Structure-function relationship of the small GTPase rab5.

1993 • 208 citations

The effect of Mg2+ on the guanine nucleotide exchange rate of p21N-ras.

1986 • 203 citations

Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung.

1993 • 161 citations

Regulatory Role for GTP-Binding Proteins in Endocytosis

1989 • 138 citations

rab GTP-binding proteins with three different carboxyl-terminal cysteine motifs are modified in vivo by 20-carbon isoprenoids.

1992 • 136 citations

Hydrolysis of GTP by Sec4 protein plays an important role in vesicular transport and is stimulated by a GTPase-activating protein in Saccharomyces cerevisiae.

1992 • 132 citations

Kinetic analysis of the hydrolysis of GTP by p21N-ras. The basal GTPase mechanism.

1988 • 130 citations

The Oncogenic Activation of Human p21 ^ras by a Novel Mechanism

1986 • 130 citations

C-terminal Truncation of p21H Preserves Crucial Kinetic and Structural Properties

1989 • 112 citations

Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic.

1990 • 100 citations

Mutants of Rab3A analogous to oncogenic Ras mutants. Sensitivity to Rab3A-GTPase activating protein and Rab3A-guanine nucleotide releasing factor

1993 • 92 citations

Mutations of the ras gene product p21 that abolish guanine nucleotide binding.

1986 • 91 citations

Role of the C-terminal region of smg p25A in its interaction with membranes and the GDP/GTP exchange protein.

1991 • 87 citations

Ras (CXXX) and Rab (CC/CXC) prenylation signal sequences are unique and functionally distinct.

1992 • 72 citations

Post-translational processing of Schizosaccharomyces pombe YPT proteins.

1992 • 68 citations

Viral Harvey ras p21 expressed in Escherichia coli purifies as a binary one-to-one complex with GDP.

1985 • 67 citations

Characterization of the mechanism of endocytic vesicle fusion in vitro.

1990 • 51 citations

Biochemical analysis of rab9, a ras-like GTPase involved in protein transport from late endosomes to the trans Golgi network.

1993 • 44 citations

The N-terminal domain of a rab protein is involved in membrane-membrane recognition and/or fusion.

1994 • 41 citations

Interactions of the ras-like protein p25rab 3A with Mg2+ and guanine nucleotides

1992 • 38 citations

Prenylation of Rab5 is dependent on guanine nucleotide binding.

1993 • 38 citations

The sorting and segregation mechanism of the endocytic pathway is functional in a cell-free system.

1990 • 32 citations

Comparison of the biochemical properties of unprocessed and processed forms of the small GTP‐binding protein, rab6p

1993 • 17 citations

Compartmentalization of low molecular mass GTP-binding proteins among neutrophil secretory granules.

1990 • 14 citations

Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli.

1992 • 13 citations

Conformational changes occurring in N‐ras p21 in response to binding of guanine nucleotide and metal ions probed by proteolysis performed under controlled conditions

1989 • 6 citations