The Active Site of the Ribosome Is Composed of Two Layers of Conserved Nucleotides with Distinct Roles in Peptide Bond Formation and Peptide Release

Catalysis in chemistry and enzymology

1969 • 3,236 citations

The Structural Basis of Ribosome Activity in Peptide Bond Synthesis

2000 • 2,195 citations

Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

1987 • 2,098 citations

Cell-free translation reconstituted with purified components

2001 • 1,788 citations

RNA Chaperones and the RNA Folding Problem

1995 • 702 citations

[25] Ribbons

1997 • 511 citations

The Crystal Structure of Human Eukaryotic Release Factor eRF1—Mechanism of Stop Codon Recognition and Peptidyl-tRNA Hydrolysis

2000 • 456 citations

Sequence elements outside the hammerhead ribozyme catalytic core enable intracellular activity

2003 • 439 citations

Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes

1986 • 425 citations

[27] Extension inhibition analysis of translation initiation complexes

1988 • 410 citations

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis

2002 • 367 citations

Mutations in the highly conserved GGQ motif of class 1 polypeptide release factors abolish ability of human eRF1 to trigger peptidyl-tRNA hydrolysis

1999 • 352 citations

On the role of magnesium ions in RNA stability

1998 • 342 citations

Induced fit in initial selection and proofreading of aminoacyl-tRNA on the ribosome

1999 • 332 citations

An RNA Switch at the 5′ Splice Site Requires ATP and the DEAD Box Protein Prp28p

1999 • 330 citations

Functional sites of interaction between release factor RF1 and the ribosome.

2000 • 297 citations

Escherichia coli ribosomes translate in vivo with variable rate.

1984 • 257 citations

A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

1995 • 238 citations

A cryo-electron microscopic study of ribosome-bound termination factor RF2

2003 • 235 citations

Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide

2001 • 202 citations

Base-Pairing between 23S rRNA and tRNA in the Ribosomal A Site

1999 • 200 citations

Bacterial Polypeptide Release Factor RF2 Is Structurally Distinct from Eukaryotic eRF1

2001 • 200 citations

Structure of the Escherichia coli ribosomal termination complex with release factor 2

2003 • 199 citations

Release of Peptide Promoted by the GGQ Motif of Class 1 Release Factors Regulates the GTPase Activity of RF3

2002 • 193 citations

Functional Association of U2 snRNP with the ATP-Independent Spliceosomal Complex E

2000 • 192 citations

Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit

2001 • 190 citations

[46] Antibiotic resistance mutations in ribosomal RNA genes of Escherichia coli

1988 • 187 citations

The Structural Basis of Large Ribosomal Subunit Function

2003 • 162 citations

Important Contribution to Catalysis of Peptide Bond Formation by a Single Ionizing Group within the Ribosome

2002 • 161 citations

Class-1 translation termination factors: invariant GGQ minidomain is essential for release activity and ribosome binding but not for stop codon recognition

2001 • 151 citations

Dominant lethal mutations in a conserved loop in 16S rRNA.

1990 • 139 citations

The Critical Role of the Universally Conserved A2602 of 23S Ribosomal RNA in the Release of the Nascent Peptide during Translation Termination

2003 • 124 citations

The essential role of the invariant GGQ motif in the function and stability in vivo of bacterial release factors RF1 and RF2

2002 • 119 citations

A conformational change in the ribosomal peptidyl transferase center upon active/inactive transition

2001 • 118 citations

A cold-sensitive mutation in 16S rRNA provides evidence for helical switching in ribosome assembly.

1993 • 102 citations

Energetic contribution of tRNA hybrid state formation to translocation catalysis on the ribosome.

2000 • 99 citations

Mutants of the Bacteriophage MS2 Coat Protein That Alter Its Cooperative Binding to RNA

1995 • 93 citations

Defining the Structural Requirements for a Helix in 23 S Ribosomal RNA that Confers Erythromycin Resistance

1989 • 87 citations

The inactivation and reactivation of ribosomal-peptidyl transferase of E. coli

1968 • 82 citations

Preorganization and protein dynamics in enzyme catalysis

2002 • 70 citations

Mutational analysis of conserved nucleotides in a self-splicing group I intron

1990 • 69 citations

After the ribosome structures: How does peptidyl transferase work?

2003 • 66 citations

pH-dependent conformational flexibility within the ribosomal peptidyl transferase center.

2001 • 62 citations

The P5abc Peripheral Element Facilitates Preorganization of the Tetrahymena Group I Ribozyme for Catalysis

2000 • 62 citations

Affinity Purification of Ribosomes with a Lethal G2655C Mutation in 23 S rRNA That Affects the Translocation

2003 • 59 citations

The G2447A mutation does not affect ionization of a ribosomal group taking part in peptide bond formation

2003 • 50 citations

Amplifying Mechanisms of Lymphomagenesis

2002 • 47 citations

Mapping Functionally Important Motifs SPF and GGQ of the Decoding Release Factor RF2 to the Escherichia coli Ribosome by Hydroxyl Radical Footprinting

2003 • 45 citations

Peptide bond formation on the ribosome: structure and mechanism

2003 • 43 citations

Mutagenesis of the peptidyltransferase center of 23S rRNA: the invariant U2449 is dispensable

2001 • 42 citations

A Core Folding Model for Catalysis by the Hammerhead Ribozyme Accounts for Its Extraordinary Sensitivity to Abasic Mutations

1998 • 42 citations

The formation of dipeptides from amino acids and the 2′(3′)-glycyl ester of an adenylate

1979 • 40 citations

The Path to Perdition Is Paved with Protons

2002 • 35 citations

Ribosome-catalyzed Pep tide-bond Formation

1995 • 32 citations

Direct three-dimensional localization and positive identification of RNA helices within the ribosome by means of genetic tagging and cyro-electron microscopy

1999 • 30 citations

Peptide‐Bond Formation on the Ribosome

1974 • 21 citations