The Xenopus Cdc6 Protein Is Essential for the Initiation of a Single Round of DNA Replication in Cell-Free Extracts

A Laboratory manual

1989 • 6,833 citations

Checkpoints: Controls That Ensure the Order of Cell Cycle Events

1989 • 3,036 citations

Chapter 30 Cell Cycle Extracts

1991 • 953 citations

Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs

1986 • 622 citations

Two steps in the assembly of complexes at yeast replication origins in vivo

1994 • 566 citations

Nuclear reconstitution in vitro: Stages of assembly around protein-free DNA

1987 • 550 citations

Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.

1987 • 510 citations

Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes

1991 • 493 citations

The nuclear lamins

1986 • 488 citations

The fission yeast cdc18+ gene product couples S phase to START and mitosis

1993 • 446 citations

Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a ‘reductional’ anaphase in the budding yeast Saccharomyces cerevisiae.

1995 • 402 citations

Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex

1994 • 393 citations

Protein-DNA interactions at a yeast replication origin

1992 • 380 citations

S-phase-promoting cyclin-dependent kinases prevent re-replication by inhibiting the transition of replication origins to a pre-replicative state

1995 • 366 citations

An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast

1996 • 362 citations

Purification of an MCM-containing complex as a component of the DNA replication licensing system

1995 • 361 citations

ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome

1995 • 357 citations

Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: Studies in Xenopus

1990 • 354 citations

Cell cycle regulation of a Xenopus Wee1-like kinase.

1995 • 316 citations

Sequential function of gene products relative to DNA synthesis in the yeast cell cycle

1976 • 302 citations

Identification of the yeast MCM3-related protein as a component of xenopus DNA replication licensing factor

1995 • 280 citations

EUKARYOTIC DNA REPLICATION: Anatomy of An Origin

1993 • 280 citations

p65cdc18 Plays a major role controlling the initiation of DNA replication in fission yeast

1995 • 259 citations

The multidomain structure of Orc1 p reveals similarity to regulators of DNA replication and transcriptional silencing

1995 • 259 citations

Rules to replicate by

1994 • 252 citations

Activation of human cyclin-dependent kinases in vitro.

1992 • 242 citations

Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis

1985 • 227 citations

MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells

1995 • 220 citations

Role for a Xenopus Orc2-related protein in controlling DNA replication

1996 • 207 citations

Early events in DNA replication require cyclin E and are blocked by p21CIP1.

1995 • 197 citations

Replication occurs at discrete foci spaced throughout nuclei replicating in vitro

1989 • 192 citations

Negative regulation of the weel protein kinase by direct action of the nim1/cdr1 mitotic inducer

1993 • 184 citations

Chapter 17 Systems for the Study of Nuclear Assembly, DNA Replication, and Nuclear Breakdown in Xenopus laevis Egg Extracts

1991 • 180 citations

Two distinct mechanisms for negative regulation of the Wee1 protein kinase.

1993 • 178 citations

The role of MCM/P1 proteins in the licensing of DNA replication.

1996 • 176 citations

REGULATION OF EUKARYOTIC DNA REPLICATION

1994 • 170 citations

DNA synthesis in a cell-free system from Xenopus eggs: Priming and elongation on single-stranded DNA in vitro

1982 • 147 citations

Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division.

1992 • 143 citations

Distinct roles of cdk2 and cdc2 in RP-A phosphorylation during the cell cycle

1993 • 136 citations

Identification of nuclear pre-replication centers poised for DNA synthesis in Xenopus egg extracts: immunolocalization study of replication protein A.

1992 • 134 citations

The role of MCM/P1 proteins in the licensing of DNA replication

1996 • 131 citations

Cip1 blocks the initiation of DNA replication in Xenopus extracts by inhibition of cyclin-dependent kinases

1994 • 130 citations

cdc18+ regulates initiation of DNA replication in Schizosaccharomyces pombe.

1996 • 129 citations

Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts.

1994 • 124 citations

Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors.

1995 • 123 citations

The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts

1995 • 119 citations

Rum1 and Cdc18 link inhibition of cyclin-dependent kinase to the initiation of DNA replication in Schizosaccharomyces pombe.

1996 • 96 citations

An analysis of the regulation of DNA synthesis by cdk2, Cip1, and licensing factor.

1995 • 79 citations

DNA replication occurs at discrete sites in pseudonuclei assembled from purified DNA in vitro

1991 • 76 citations

Molecular Cloning of Saccharomyces cerevisiae CDC6 Gene

1989 • 60 citations

Eukaryotic replicators and associated protein complexes

1995 • 46 citations

Cloning and characterization of theSaccharomyces cerevisiaeCDC6 gene

1988 • 41 citations

In vitro cell cycle arrest induced by using artificial DNA templates.

1992 • 41 citations

The chromatin structure of Saccharomyces cerevisiae autonomously replicating sequences changes during the cell division cycle.

1991 • 34 citations

DNA replication: Controlling initiation during the cell cycle

1996 • 31 citations

Eukaryotic DNA replication

1995 • 31 citations

The initiation of DNA replication in the budding yeast cell division cycle

1995 • 21 citations