Generation, validation, and large scale production of adenoviral recombinants with large size inserts such as a 6.3 kb human dystrophin cDNA

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5

1977 • 4,801 citations

Complete cloning of the duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals

1987 • 2,353 citations

Rapid transfer of DNA from agarose gels to nylon membranes

1985 • 1,810 citations

Isolation of High‐Molecular‐Weight DNA from Mammalian Cells

1973 • 1,723 citations

Transfecting Mammalian Cells: Optimization of Critical Parameters Affecting Calcium-Phosphate Precipitate Formation

1996 • 862 citations

An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3.

1994 • 743 citations

Adenovirus-mediated gene transfer transiently corrects the chloride transport defect in nasal epithelia of patients with cystic fibrosis

1993 • 669 citations

Very mild muscular dystrophy associated with the deletion of 46% of dystrophin

1990 • 578 citations

A new adenoviral vector: Replacement of all viral coding sequences with 28 kb of DNA independently expressing both full-length dystrophin and beta-galactosidase.

1996 • 572 citations

Human dystrophin expression in mdx mice after intramuscular injection of DNA constructs

1991 • 486 citations

Packaging capacity and stability of human adenovirus type 5 vectors

1993 • 457 citations

Efficient adenovirus-mediated transfer of a human minidystrophin gene to skeletal muscle of mdx mice

1993 • 455 citations

Development of adenovirus vectors for the expression of heterologous genes.

1988 • 386 citations

Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1–deleted adenoviruses

1993 • 303 citations

Adenovirus as an expression vector in muscle cells in vivo.

1992 • 301 citations

Definition of an efficient synthetic poly(A) site.

1989 • 285 citations

Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit β-globin mRNA 3′ end formation

1987 • 229 citations

The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2

1992 • 214 citations

A differential efficiency of adenovirus-mediated in vivo gene transfer into skeletal muscle cells of different maturity

1994 • 209 citations

Long–term correction of mouse dystrophic degeneration by adenovirus–mediated transfer of a minidystrophin gene

1993 • 201 citations

Emergence of Early Region 1-Containing Replication-Competent Adenovirus in Stocks of Replication-Defective Adenovirus Recombinants (ΔE1 + ΔE3) During Multiple Passages in 293 Cells

1994 • 193 citations

Expression of Heterologous Sequences in Adenoviral Vectors

1992 • 171 citations

Gene Transfer and Expression Protocols

1991 • 165 citations

Dystrophin Expression in Muscles of mdx Mice After Adenovirus-MediatedIn VivoGene Transfer

1996 • 149 citations

Generation of adenovirus by transfection of plasmids

1983 • 144 citations

Gene therapy using adenoviral vectors

1994 • 134 citations

Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells

1994 • 130 citations

Gene Therapy for Cystic Fibrosis Using E1-Deleted Adenovirus: A Phase I Trial in the Nasal Cavity. University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

1994 • 119 citations

Detection of wild-type contamination in a recombinant adenoviral preparation by PCR.

1995 • 115 citations

Development of a helper-independent human adenovirus vector and its use in the transfer of the herpes simplex virus thymidine kinase gene

1986 • 113 citations

Protection of cattle from BHV-1 infection by immunization with recombinant glycoprotein gIV

1993 • 112 citations

Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis.

1993 • 111 citations

Transient immunosuppression by FK506 permits a sustained high-level dystrophin expression after adenovirus-mediated dystrophin minigene transfer to skeletal muscles of adult dystrophic (mdx) mice.

1996 • 106 citations

Adenovirus-mediated gene transfer

1993 • 90 citations

Efficient selection of recombinant adenoviruses by vectors that express beta-galactosidase

1995 • 84 citations

Animal Cell Technology: Developments Towards the 21st Century

1995 • 80 citations

Evaluation of the Efficacy and Safety ofIn Vitro, Adenovirus-Mediated Transfer of the Human Cystic Fibrosis Transmembrane Conductance Regulator cDNA

1994 • 80 citations

Safety of Adenovirus-Mediated Transfer of the Human Cystic Fibrosis Transmembrane Conductance Regulator cDNA to the Lungs of Nonhuman Primates

1996 • 74 citations

Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies

2000 • 72 citations

Helper-Free Adenovirus Type 5 Vectors

1982 • 61 citations

Herpes simplex virus IgG Fc receptors induced using recombinant adenovirus vectors expressing glycoproteins E and I

1990 • 59 citations

Abundant expression of polyomavirus middle T antigen and dihydrofolate reductase in an adenovirus recombinant

1987 • 53 citations

Improved Adenovirus Vector Provides Herpes Simplex Virus Ribonucleotide Reductase R1 and R2 Subunits Very Efficiently

1995 • 45 citations

Constitutive episomal expression of polypeptide IX (pIX) in a 293-based cell line complements the deficiency of pIX mutant adenovirus type 5

1995 • 40 citations

Adenovirus-mediated gene transfer into striated muscles

1995 • 38 citations

High Level Expression in 293 Cells of the Herpes Simplex Virus Type 2 Ribonucleotide Reductase Subunit 2 Using an Adenovirus Vector

1990 • 28 citations

An efficient procedure to select and recover recombinant adenovirus vectors.

1995 • 27 citations

Recombinant Truncated Dystrophin Minigenes: Construction, Expression, and Adenoviral Delivery

1995 • 25 citations

Gene Therapy: The Advent of Adenovirus

1994 • 23 citations

Gene transfer and expression protocols

1992 • 21 citations