Probing β‐lactamase structure and function using random replacement mutagenesis

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

A Laboratory manual

1989 • 6,833 citations

The structure of β-lactamases

1980 • 1,705 citations

A RAPID ALKALINE EXTRACTION PROCEDURE FOR SCREENING RECOMBINANT DNA

1979 • 1,402 citations

Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine.

1965 • 943 citations

Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

1978 • 791 citations

Structure and function of haemoglobin

1965 • 603 citations

Penicillinase Synthesis Controlled By Infectious R Factors In Enterobacteriaceae

1965 • 555 citations

Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions

1990 • 485 citations

Structure and function of haemoglobin

1965 • 440 citations

Alternative packing arrangements in the hydrophobic core of λrepresser

1989 • 375 citations

Bacterial Resistance to β-Lactam Antibiotics: Crystal Structure of β-Lactamase from Staphylococcus aureus PC1 at 2.5 Å Resolution

1987 • 349 citations

Receptor and Antibody Epitopes in Human Growth Hormone Identified by Homolog-Scanning Mutagenesis

1989 • 345 citations

The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 <scp>dd</scp>-peptidase family

1988 • 334 citations

Combinatorial Cassette Mutagenesis as a Probe of the Informational Content of Protein Sequences

1988 • 326 citations

Secretion of beta-lactamase requires the carboxy end of the protein

1980 • 311 citations

Characterization of beta-lactamases

1989 • 302 citations

Refined crystal structure of β-lactamase from Citrobacter freundiiindicates a mechanism for β-lactam hydrolysis

1990 • 299 citations

On the Origin of Bacterial Resistance to Penicillin: Comparison of a β-Lactamase and a Penicillin Target

1986 • 223 citations

.beta.-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin

1980 • 222 citations

Refined crystal structure of β-lactamase from Staphylococcus aureus PC1 at 2.0 Å resolution

1991 • 217 citations

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

1982 • 211 citations

In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

1978 • 200 citations

Synthetic lacoperator DNA is functional in vivo

1976 • 196 citations

GENETIC ANALYSIS OF STAPHYLOCOCCAL NUCLEASE: IDENTIFICATION OF THREE INTRAGENIC "GLOBAL" SUPPRESSORS OF NUCLEASE-MINUS MUTATIONS

1985 • 189 citations

β‐Lactamase of Bacillus licheniformis 749/C at 2 Å resolution

1990 • 175 citations

Genetic studies of the lac repressor

1990 • 173 citations

Rapid purification of plasmid DNA by a single centrifugation in a two-step cesium chloride-ethidium bromide gradient

1983 • 161 citations

β-LACTAMASES

1984 • 159 citations

The crystal structure of the β-lactamase of Streptomyces albus G at 0.3 nm resolution

1987 • 149 citations

Site-directed mutants, at position 166, of RTEM-1 beta-lactamase that form a stable acyl-enzyme intermediate with penicillin.

1991 • 137 citations

An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase.

1989 • 134 citations

Tertiary structural similarity between a class A β-lactamase and a penicillin-sensitive D-alanyl carboxypeptidase-transpeptidase

1986 • 134 citations

An X-ray-crystallographic study of β-lactamase II from Bacillus cereus at 0.35 nm resolution

1987 • 102 citations

Substitution of lysine at position 104 or 240 of TEM-1pTZ18R .beta.-lactamase enhances the effect of serine-164 substitution on hydrolysis or affinity for cephalosporins and the monobactam aztreonam

1991 • 97 citations

A single glutamic acid residue plays a key role in the transcriptional activation function of lambda repressor

1989 • 94 citations

Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties

1985 • 91 citations

The role of lysine-234 in .beta.-lactamase catalysis probed by site-directed mutagenesis

1990 • 87 citations

Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.

1982 • 84 citations

Characterization of the plasmid genes blaT-4 and blaT-5 which encode the broad-spectrum β-lactamases TEM-4 and TEM-5 in Enterobacteriaceae

1989 • 81 citations

Functionally acceptable substitutions in two α‐helical regions of λ repressor

1990 • 76 citations

Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71.

1986 • 73 citations

β-lactamase I from Bacillus cereus. Structure and site-directed mutagenesis

1987 • 69 citations

Mutants generated by the insertion of random oligonucleotides into the active site of the .beta.-lactamase gene

1989 • 68 citations

Defining the consensus sequences ofE.colipromoter elements by random selectio

1988 • 60 citations

Patterns and mechanisms of beta-lactam resistance among isolates of Escherichia coli from hospitals in the United States

1990 • 55 citations

Sequence analysis and evolutionary perspectives of ROB-1 beta-lactamase

1990 • 48 citations

Substitution of serine for arginine in position 162 of TEM-type β-lactamases extends the substrate profile of mutant enzymes, TEM-7 and TEM-101, to ceftazidime and aztreonam

1989 • 46 citations

Excitement in the β-lactamase arena

1989 • 28 citations

Improved peptide function from random mutagenesis over short ‘windows’

1988 • 26 citations

Mutational analysis of the cloned chicken thymidine kinase gene.

1983 • 15 citations

Precise nucleotide sequence modifications with bidirectionally cleaving class-IIS excision linkers

1987 • 13 citations